Showing papers in "Experimental Parasitology in 2009"

TL;DR: Overall, the widespread distribution of pathogenic Acanthamoeba T4 across the environmental sources and the increasing number of contact lens wearers in Iran, demands more awareness within the public and health professionals as this pathogen is emerging as a risk for human health in Iran and worldwide.

TL;DR: The results suggest that T. gondii can be sexually transmitted in domestic dogs and several T. Gondii cysts were detected in the brains of four offspring.

TL;DR: Flow cytometry as described here allows parasitemia and parasites of different stages to be quantified according to the DNA content and the proportion of parasitized erythrocytes estimated by FCM and the Giemsa method agreed with determination by parasite lactate dehydrogenase.

TL;DR: This is the first study, which applies the LAMP method to diagnose Toxoplasma from veterinary samples, and it has a potential to be applied as an alternative molecular diagnostic tool for detection of T. gondii infection from Veterinary samples.

TL;DR: D duplex real-time PCR allows the detection of malarial cases, including mixed species infection, it simplifies analysis and reduces cost, and may prove invaluable for use in the diagnosis of human infection, trial treatments and epidemiologic studies in which high-throughput analyses are often required.

TL;DR: Cyt b gene analysis is applicable to the analyzing phylogeny of the genus Leishmania and may be useful for separating non-human pathogenic species from human pathogenicspecies.

TL;DR: It is demonstrated that the PCR assay established provides a potential tool for the early diagnosis and therapy evaluation for S. japonicum infection in humans.

TL;DR: Treated A. castellanii trophozoites, intact cysts and cyst walls contained various sugar moieties, of which a high percentage was galactose and glucose, in addition to small amounts of mannose, and xylose.

TL;DR: The subtypes identified have global distributions and indicate that anthroponotic and zoonotic transmission routes contribute to sporadic human cryptosporidiosis in NSW.

TL;DR: PCR sensitivity to detect T. evansi using the primers 21/22-mer, ITS1, ESAG 6/7 and TBR 1/2 designed from repetitive (multicopies) genomic sequences is evaluated, showing TBR1/2 and ESAG6/7 were the best primers to monitor T.evansi infections in mice.

TL;DR: A high-resolution multiplex PCR assay was developed to distinguish T. asiatica infections from infection with other human Taenia tapeworms and was useful for the differentially diagnosing T. saginata and T. solium.

TL;DR: Development of this lineage of P. relictum is investigated in experimentally infected greenfinches Carduelis chloris and the obtained data is compared with the literature information about the virulence of the same parasite lineage for phylogenetically closely related bird species.

TL;DR: In this paper, the causative agent of infantile visceral leishmaniasis (IVL) was found to predominate in all foci of IVL in Iran, based on PCR detection and sequencing of parasite Cysteine Protease B.

TL;DR: A total of 297 pig faecal samples were screened for the presence of Giardia by PCR and genotyped and sequence analysis identified assemblage A and E in pre- and post-weaned pigs.

TL;DR: It is concluded that phosphoglycan assembly and expression mediated by L. donovani LPG2 are important for promastigote and amastigotes virulence, unlike L. mexicana but similar to L. major.

TL;DR: The results indicate that A. annua infusion is useful to control T. gondii infection, due to its low toxicity and its inhibitory action directly against the parasite, resulting in a well tolerated therapeutic tool.

TL;DR: It was found that toxoplasmosis can affect main reproductive parameters in male rats, which are the most predictive of their fertilizing capacity.

TL;DR: The results of experimental transmission showed that the larval, nymph and adult stages of H. a.

TL;DR: Results suggest that, similar to ROP2, rhoptry protein ROP4 could be a very good candidate for future anti-T.

TL;DR: RNA analysis from resistant (BS-90), non-susceptible (LAC2) and susceptible (NMRI) juvenile Biomphalaria glabrata to Schistosoma mansoni revealed that stress-related genes, heat shock protein 70 (Hsp 70) and reverse transcriptase (RT), were dramatically co-induced early in susceptible snails, but not in resistant/non-sUSceptible ones.

TL;DR: Promastigotes of Leishmania (Viannia) panamensis were successfully transfected with p6.5-egfp to express green fluorescent protein, providing an in vitro model for screening antileishmanial drugs and facilitates drug discovery program for the Viannia species, which differ significantly from those of the Leishania subgenus.

TL;DR: This work can be used as a protocol with other chemicals, to determinate the LC(50), basic procedure for studies of control, resistance and behavior of ticks treated with acaricides, especially the brown dog tick R. sanguineus.

TL;DR: The results show the inability of the T. cruzi IIb clones to infect mammalian cells, produce trypomastigotes and replicate in Rhodnius prolixus, the main vector species in Colombia, and suggest that the high prevalence of T. Cruzi I in Colombia results from more successful interactions between parasite lineage, vector, and host species.

TL;DR: A comparative proteomic analysis of a genetically related pair of antimonial-sensitive and -resistant Leishmania infantum axenic amastigote strains highlights several novel proteins that are putatively involved in resistance to antimonials.

TL;DR: Thionins resulted as the most active peptides tested until now, and were associated to permeabilization of the plasma membrane leading to an immediate death of the parasite.

TL;DR: It is proposed that infection of BMDMPhi with Leishmania promastigotes activates both positive and negative signaling pathways that control IL-12 production, and activation of PI3K/Akt signaling had differential effects on ERK and p38 activation.

TL;DR: Nine novel types of immunogenic proteins that might be potential candidates of vaccine development for toxoplasmosis are obtained, which will confirm in later studies.

TL;DR: It is shown that HDAC activity is necessary for the transformation of S. mansoni miracidia during infection of the snail host and that TSA reversibly blocks this developmental process.

TL;DR: The high antiparasitic activity of PS-1, along with the unrelatedness of its chemical structure to any present antimicrobial drug, which prevents the development of cross-resistance, together with its non-toxicity to mammalian cells make this peptide a promising candidate for the treatment of malaria and leishmaniasis.

TL;DR: Examination of mucosal secretions from 114 individuals of 37 countries, inhabitants and/or visitors, aged 16-65 years in London, UK found that Acanthamoeba antibody prevalence rate was more than 85%, without any significant differences between males (86.2%) and females (89.2%).