Showing papers in "FEBS Letters in 1975"

TL;DR: The cell envelope of Enterobacteriaceae consists of two membranes separated by a peptidoglycan layer, and methods have been developed to separate the cytoplasmic membrane from the outer membrane.

TL;DR: The object of this letter is to define the general principles of the protonmotive Q cycle more explicitly than before, thus facilitating either its experimental rejection or its further development and general application.

TL;DR: The protonmotive Q* cycle is described, both for explaining the protontranslocating function of the cytochrome b-cl segment of the respiratory chain, and for rationalising much of the biochemical information about the behaviour and functions of the b cytochromes and ubiquinone, which previously appeared to be difficult to interpret simply.

TL;DR: This paper reports on the complete amino acid sequence of human serum albumin, including the positions of all amides; the results presented are confronted with sequential data reported from other laboratories.

TL;DR: These radical anions dismutate to Hz O2 either nonenzymatically or by the reaction catalyzed by superoxide dismutase, according to the MC Cord Fridovich reaction.

TL;DR: It is shown that one protein component of mol.

TL;DR: Since glutamine PRPP amidotransferase activity was increased but the opposing enzyme, xanthine oxidase, was decreased in all the hepatomas, the reprogramming of gene expression results in an imbalance that favors synthesis against catabolism, which should confer selective advantages to the cancer cells.

TL;DR: A lectin in seeds of viciu ervilia is found, which has been purified and partially characterized and has been submitted to other investigators for examination of its possible utility as a biospecific adsorbent, especially for virus purification.

TL;DR: A rapid, sensitive and accurate assay for DNA in as few as 10’ cells (or 0.5 pg of pure DNA) is developed, based on the fact that the antibiotic mithramycin binds to double stranded DNA and fluoresces in direct proportion to the amount of DNA present.

TL;DR: A preliminary characte- rization of the isolated protein, which is tentatively identified with the ADP,ATP carrier, will be presented and a brief account of a new simple purification method which is a modification of that previously published.

TL;DR: The results of the first studies on benzo(u)pyrene undertaken with the construction of a highly sensitive spectrophotofluorometer are reported, in which the fluorescence spectral characteristics of DNA from benzo (a)pyrenetreated mouse skin have been compared with those of DNA treated in solution with reactive Benzo(a) pyrene derivatives.

TL;DR: A soluble lipoxygenase is described in rabbit reticulocytes with the ability to cause lysis of intact mitochondria by peroxidation of their lipids and pure phospholipids are also attacked.

TL;DR: Suggestions are that in oxidative phosphorylation and photophosphorylation, the requisite conformational changes may be coupled to exposure of charged groups to different sides of the membrane, and similar conformational coupling suggestions may explain proton translocation coupled to ATP cleavage and active transport of metabolites coupled to membrane potential.

TL;DR: Evidence is obtained that the tonA protein is involved in ferrichrome dependent iron uptake, besides that of being the receptor for killing agents, in E. coli K12 ROW.

TL;DR: Results obtained by an independent method for measuring cyclic-AMP stimulated cyclic -AMP release are reported: prelabelling of cells with [3H]adenine and chromatographic separation of the labelled compounds from the extracellular fluid after stimulation of the cells by unlabelled cyclic AMP.

TL;DR: The application of different pulse sequences for the simplification of complex nmr spectra of lysozyme and myoglobin is discussed and resonances may be observed selectively because of intrinsic differences in relaxation times or in multiplet structure and coupling constants.

TL;DR: The outstanding heat stability of the basic pancreatic trypsin inhibitor made it possible in the present experiments to observe the NMR of the aromatic protons in the globular protein conformation over the temperature range from 4°C to 5°C.

TL;DR: The hypothesis that the ‘inner’ carnitine acyltransferase is vectorial and catalyses a direct acyl transfer between external carnite and matrix CoA is suggested.

TL;DR: A new 1,3; 1,4-/Iglucan with these properties has been prepared and its use in studies on the specificity of /3- glucan endohydrolases is described.

TL;DR: It was thought to be of interest to examine whether DNAase I would effect the polymerization of G-actin and conversely whether polymerized actin (F- actin) would interact with DNAaseI, since actin is found in the filamentous form in situations involving directed motion.

TL;DR: The introduction of a new gradient medium such as metrizamide, the properties of which differ markedly from those of other density-gradient materials, represents a significant advance in the application of isopycnic centrifugation to the frac- tionation of macromolecules, subcellular components, viruses and cells.

TL;DR: It was proposed that Signal IIf arises from Z.

TL;DR: Results are presented which show that when perfused rat heart is stimulated by 1 O6 M adrenaline there is a time dependent increase in phosphorylation of troponin-I which coincides with the increase in force of contraction.

TL;DR: The complete amino acid sequence of the polypeptide has now been elucidated, and the present paper gives the evidence for the proposed sequence.

TL;DR: It is suggested that the radical-like agent responsible for the peroxidation and lysis of purified lysosomes, incubated with xanthine,xanthine oxidase and chelated iron, is the hydroxyl radical formed from 0; according to reaction (2).

TL;DR: Alternative procedures of reconstitution in which both sonication and dialysis are avoided are developed, and one of these, which the authors refer to as a cholate-dilution procedure, is suitable for the reconstitutes of several membrane systems.

TL;DR: In this paper, it was shown that neutral red, a nitrogenous pH-indicator with a pK of 6.8, responds with spectral shifts which indicate a net acidification to illumination of chloroplasts.

TL;DR: Three toxic polypeptides (toxins I, II and III) were isolated in analytically pure form using alcoholic extracts of whole animals, batchwise adsorption on cation exchangers, gel filtration and ionexchange chromatography, and the neurotoxic activity was tested on the shore crab Carcinus maenas.