scispace - formally typeset
Search or ask a question

Showing papers in "FEBS Letters in 1976"


Journal ArticleDOI
TL;DR: The membrane potential has been suggested to be the major component of the electrochemical proton gradient under most conditions, and measurements of this potential are therefore essential for the understanding of the mechanism of oxidative phosphorylation and mitochondrial ion transport.

730 citations


Journal ArticleDOI
TL;DR: The experiments reported in this paper demonstrate that MG236A and ML-236B inhibit specifically 3-hydroxy-3-methylglutaryl (HMG)CoA reductase (EC 1 .I .1.34), the rate-limiting enzyme in cholesterol synthetic pathway, without affecting the rest of the enzymes involved in this pathway, and that the inhibition is competitive with respect to the substrate HMG-CoA.

699 citations


Journal ArticleDOI
TL;DR: The present study was undertaken to determine the structure and synthetic pathway of HbAfc, and indicated the bond to be a Schiff base which, however, proved stable even at pH 4.0.

505 citations


Journal ArticleDOI
TL;DR: In the present study heparin was separated by affinity chromatography on antithrombin-substituted Sepharose into two distinct fractions, one with high affinity and one with little or no affinity for the protein.

401 citations


Journal ArticleDOI
TL;DR: Tunicamycin, a N-acetylglucosamine containing antibiotic from Streptomyces lysosuperijkus was originally isolated on the basis of its antiviral activity but subsequent work demonstrated that tunicamycin selectively interferes with glycoprotein biosynthesis.

341 citations


Journal ArticleDOI
TL;DR: It was found that glycopeptides possessing two peripheral NeuNAcGal-GlcNAc*-branches linked to the core pentasaccharide were bound by the lectin, whereas glycopePTides with three branches were not (the structures of these compounds are shown below).

253 citations


Journal ArticleDOI
TL;DR: The results of these experiments show that the rate limiting step for the recovery of photoactivity in Photosystem II units is a function of the oxidation state of the S enzyme, indicating that reactions occurring on the reducing side of photosystem II are ratelimiting.

235 citations


Journal ArticleDOI
TL;DR: The preliminary experiments reported here demonstrate the possibility of oral administration of insulin by such encapsulation within Liposomes, and when liposomes containing entrapped insulin were given orally to diabetic rats, there was a significant reduction of the bloodglucose level, whereas the same amount of free insulin had no effect on the blood-glucoses level.

226 citations


Journal ArticleDOI
TL;DR: The crystal structure of a neurotoxin from the sea snake Laticauda semifasciata, a ‘short’ neurotoxin of 62 amino acids cross-linked by four disulfide bridges, is solved and it is concluded that this structure is common to all snake venom neurotoxins, and that the postsynaptic toxins act by inserting the loop into a cleft or channel in the acetylcholine receptor.

224 citations


Journal ArticleDOI
TL;DR: It is shown that in the subcellular fraction which contains the interferoninduced inhibitor(s) of translation, there is strong inhibition of polypeptide chains in the form of protein phosphorylation.

215 citations


Journal ArticleDOI
TL;DR: The amino acid sequence of the dominating cathodic horseradish peroxidase isoenzyme consists of 308 amino acid residues, a hemin group, and 8 neutral carbohydrate side chains attached through asparagine residues.

Journal ArticleDOI
TL;DR: A hypothesis has been proposed in which phosphorylation of Troponin-I in response to catecholamine stimulation of cardiac muscle could cause an increase in contraction by modifying the properties of troponin.

Journal ArticleDOI
TL;DR: It is reported here that modulator protein possesses troponin C-like activity in Ca2+-regulated actomyosin systems from skeletal muscle and is as effective in the presence of trop onin I and tropomyos in skeleta muscle as it is in the absence of these components.

Journal ArticleDOI
TL;DR: Fingerprint and amino acid analysis of a corresponding CNBr-fragment of 63 amino acids from M and Z protein give clear evidence for an amino acid substitution, glutamic acid to lysine.

Journal ArticleDOI
TL;DR: It is reported, that low concentrations of serum activate purified human postheparin plasma hepatic lipase, and the C-apoproteins from VLDL did not cause this stimulation, but all of them suppressed the enzymatic activity.

Journal ArticleDOI
TL;DR: An improved technique for the preparation of ferritin-avidin conjugates for use as an ultrastructural marker in the localization of surface receptor sites on biotin-derivatized whole cells is described.

Journal ArticleDOI
TL;DR: The data persuade us that there is no unique protein species corresponding to L8 in the E. coli ribosome and the stability and specificity of the aggregate formed between L7/L12 and LlO suggest that these proteins are immediate neighbors bound to each other in the intact SOS subunit.

Journal ArticleDOI
TL;DR: The results reported here suggest that this is unlikely to be so, and the major difference, whose significance remains to be determined, is the shorter length of DNA contained in the nucleosome, a difference which recent results suggest may be common to the fungi.

Journal ArticleDOI
TL;DR: It is demonstrated that the current hypothesis in which cytochromes a and a3 are assumed to contribute equally to the a-band at 605 nm disagrees with spectral information, and that this band is mostly, perhaps entirely, due to cytochrome a.

Journal ArticleDOI
TL;DR: A few hours after irradiation in vivo, the cells in lymphoid tissues undergo serious changes and the pyknotic degeneration of cell nuclei appears, which later leads to so called intermitotic death of the cells.

Journal ArticleDOI
TL;DR: Evidence is reported of a modification in OLI 1 resistant mutants of one of the mitochondrially synthesized subunits of the ATPase of the oligomycinsensitive adenosine triphosphatase.


Journal ArticleDOI
TL;DR: Evidence is provided that the receptor protein for phage T6 and colicin K is a constituent of a ‘channel’ through the outer membrane because the uptake of nucleosides is severely impaired in ts.x mutants which lack an outer membrane protein with a mol.

Journal ArticleDOI
TL;DR: The DNA repeat lengths of chromatins from sea urchin sperm and gastrula cells are markedly different, but both higher than that of rat liver chromatin.

Journal ArticleDOI
TL;DR: The present paper provides a summary of the features of this group of enzymes, first discovered in bacteria and phage-infected bacteria, and the properties of the DNA ligases of higher organisms have not been previously reviewed.

Journal ArticleDOI
TL;DR: Crystallographic structural studies of the trypsin-trypsinogen-PTI system found indeed that the complex between PTI and trypsInogen crystallizes isomorphously to the tryPSinPTI complex and is structurally extremely similar.

Journal ArticleDOI
TL;DR: NMR techniques which can provide accurate measurements of the rates of rotation of amino acid side chains of proteins, which involve spectra simulation and cross-saturation are illustrated for a tyrosine residue of ferrocytochrome c.

Journal ArticleDOI
TL;DR: Energy-dependent release of adenylates tightly bound to CF, is demonstrated, indicating that chemiosmotic and conformational hypotheses may not be regarded as alternative but additive con- cepts for the comprehension of the mechanism of photophosphorylation.

Journal ArticleDOI
TL;DR: Ecdysone levels were determined by RIA in Bombix mori and in Philosamia Cynthia, another silkworm which undergoes a pupal diapause, and a single hormonal peak was found at each developmental stage.

Journal ArticleDOI
TL;DR: Experiments demonstrating light-induced changes of the electrochemical proton gradient in intact cells of Halobacterium halobium are reported.