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Showing papers in "FEBS Letters in 1996"


Journal ArticleDOI
TL;DR: A novel estrogen receptor (hereinafter referred to as ERβ) was cloned using degenerate PCR primers and revealed that ERβ is expressed in human thymus, spleen, ovary and testis, and the level of transactivation by 17β‐estradiol is higher for ERα than for ERβ, which may reflect suboptimal conditions for ER β at thelevel of the ligand, responsive element or cellular context.

2,243 citations


Journal ArticleDOI
TL;DR: The results show that the relative abilities of the carotenoids to scavenge the ABTS·+ radical cation are influenced by the presence of functional groups with increasing polarities, such as carbonyl and hydroxyl groups, in the terminal rings, as well as by the number of conjugated double bonds.

971 citations


Journal ArticleDOI
TL;DR: Results indicate that the hypothalamus is a key site of leptin action, although other brain regions are also targeted.

869 citations


Journal ArticleDOI
TL;DR: The results suggest that at least two ROCK isoforms are present in a single species and play distinct roles in Rho‐mediated signalling pathways.

757 citations


Journal ArticleDOI
TL;DR: The substrate specificity of protein kinase‐Bα (PKBα), also known as RAC kinase or Akt, was investigated using synthetic peptide substrates related to the sequence surrounding the phosphorylation site on glycogen synthase kinase (GSK3) to find the most effective substrate.

659 citations


Journal ArticleDOI
TL;DR: Contrary to the poor inhibition of PKCμ by Gö 6983, this compound was found to suppress in vitro kinase activity ofPKC isoenzymes from all three subgroups very effectively with IC50 values from 7 to 60 nM.

612 citations


Journal ArticleDOI
TL;DR: These largely non‐overlapping tissue distributions of human α‐ and β‐defensins suggest that hBD‐1 may be positioned to defend epithelial cells and mucosae from infection, whereas expression of HNP1‐3 in neutrophils and HD5 and HD6 in Paneth cells allows these α‐ defensins to participate in systemic and small intestinal host defenses, respectively.

562 citations


Journal ArticleDOI
TL;DR: This work has shown that in vitro induction of permeability transition in isolated mitochondria provokes the release of a protein factor capable of inducing nuclear chromatin condensation and fragmentation, which prevents apoptosis.

506 citations


Journal ArticleDOI
TL;DR: It is reported that PHF assembly is strongly enhanced by RNA, dependent on the formation of intermolecular disulfide bridges involving Cys322 in the third repeat of tau and includes the dimerization of t Tau as an early intermediate.

482 citations


Journal ArticleDOI
TL;DR: This is the first description of how laccase might function in a biological system for the complete depolymerization of lignin.

451 citations


Journal ArticleDOI
TL;DR: In conclusion, these data are compatible with the hypothesis that apoptotic ΔΨ m disruption is mediated by the formation of PT pores and that PT‐mediated ΔΩ mruption is a critical event of the apoptotic cascade.

Journal ArticleDOI
TL;DR: The results suggest that tyrosine nitration interferes with phosphorylation and targets proteins for degradation, and specific enzymatic process(es) for removing nitrotyrosine may be present in vivo.

Journal ArticleDOI
TL;DR: In this paper, the mitochondrial membrane potential (ΔΨ), which is the driving force for mitochondrial ATP synthesis, declines during apoptosis, and maintenance of ΔΨ prevents apoptosis.

Journal ArticleDOI
TL;DR: The results indicate that gene transfer by electroporation in vivo may avoid anatomical constraints and low transfection efficiency.

Journal ArticleDOI
TL;DR: Recent findings on genomic organization, and on the proteins and their presumed function, will be discussed, which is important for eumelanin synthesis in mouse and man.

Journal ArticleDOI
TL;DR: The results show that MMP‐13 cleaves aggrecan in the IGD at the same site (PEN341‐FFG..) identified for other members of the MMP family, and also at a novel site ..VKP384‐VFE.. not previously observed for other proteinases.

Journal ArticleDOI
TL;DR: It is proposed that ROS‐induced pore opening lowers ROS production due to maximal stimulation of mitochondrial O2 consumption and, hence, intracellular [O2] lowering and (b) complete dissipation of mitochondrial membrane potentials and, as a consequence, maximal oxidation of such respiratory chain carriers as CoQ.

Journal ArticleDOI
TL;DR: It is demonstrated that furin specifically cleaves MT1‐MMP between Arg111‐Tyr in vitro, which resulted in a stimulation of progelatinase A‐activation function.

Journal ArticleDOI
TL;DR: The biosynthesis and intracellular processing by the proprotein convertases furin, PC1, PC2, PACE4, PC5 and its isoform PC5/6‐B effectively process proBDNF and proNT3 is analyzed and it is determined that human pro NT3 is sulfated, suggesting that processing of proNT 3 occurs following the arrival of the precursor to the Trans Golgi Network.

Journal ArticleDOI
TL;DR: It is proposed that shear stress interferes with common cell death signal transduction involving the CPP32‐like protease family and may contribute to endothelial cell integrity by inhibition of apoptosis.

Journal ArticleDOI
TL;DR: The distribution of anandamide in human brain and spleen supports its potential role as an endogenous agonist in central and peripheral tissues and suggests that it is metabolized in tissues where it is synthesized, and that its action is probably not hormonal in nature.

Journal ArticleDOI
TL;DR: The results show the physiological existence of kinase porin translocator complexes at the mitochondrial surface, and it is assumed that such complexes between inner and outer membrane components are the molecular basis of contact sites observed by electron microscopy.

Journal ArticleDOI
TL;DR: Results indicate the presence of a putative nitric oxide synthase in plants, which was synthesized by roots and nodules of Lupinus albus in an l‐arginine‐dependent manner.

Journal ArticleDOI
TL;DR: The results indicate that CHOP can induce apoptosis in a p53‐independent manner and requires leucine zipper domain but neither intact basic region nor trans‐activation domain.

Journal ArticleDOI
TL;DR: COX‐2 might be regarded as the COX isozyme responsible for spinal PG release in nociceptive processing under a peripheral inflammatory stimulus.

Journal ArticleDOI
TL;DR: Transfer of MPEG1900‐DSPE from micellar phase to pre‐formed liposomes imparts long in vivo circulation half‐life to an otherwise rapidly cleared lipid composition and suggests a simple and economical way to impart site‐specific targeting to a variety of liposome delivery systems.

Journal ArticleDOI
TL;DR: Spectroscopic studies show that all the compounds chelate iron and copper; enhanced absorbance in the visible region is observed in the case of the iron‐digallate complex, but not with copper.

Journal ArticleDOI
TL;DR: The ability of Vpu to increase virus release from infected cells may be correlated with an ion channel activity of the TM domain, thereby providing a potential target for drug intervention based on the development of VPU‐specific channel blockers.

Journal ArticleDOI
TL;DR: The green fluorescent protein (GFP) of Aequorea victoria is a unique in vivo reporter for monitoring dynamic processes in cells or organisms and can be used to localize proteins, to follow their movement or to study the dynamics of the subcellular compartments to which these proteins are targeted.

Journal ArticleDOI
Juerg Rentsch1, Michele Chiesi1
TL;DR: In this article, the expression of the leptin gene product was investigated by quantitative competitive RT-PCR in a mouse cell line (3T3-L1) which can be induced to differentiate into adipocytes.