Food and Agricultural Immunology 

About: Food and Agricultural Immunology is an academic journal published by Taylor & Francis. The journal publishes majorly in the area(s): Immunoassay & Immune system. It has an ISSN identifier of 0954-0105. It is also open access. Over the lifetime, 1375 publications have been published receiving 18340 citations.

The Effect of Bee Honey on the Proliferative Activity of Human B-and T-Lymphocytes and the Activity of Phagocytes

Abstract: The effect of bee honey (BH) taken from Apis melifica on human peripheral blood lymphocytes and neutrophils was studied using lymphocyte blastogenic 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and quantitative nitroblue tetrazolium (NBT) assays, respectively. Bee honey showed a mitogenic effect on both B- and T-lymphocytes. Stimulated by lipopolysaccharide (LPS) at 0.1% BH, B-cells showed maximum stimulatory index (0.838 ± 0.14 relative to 0.521 ± 0.09). Stimulated by concanavalin A (Con A) or phytohemagglutinin (PHA) in the presence of 0.2% BH, T-cells showed maximum stimulatory index of 0.820 ± 0.12 and 0.712 ± 0.09 compared to controls of 0.531 ± 0.07 and 0.648 ± 0.08, respectively. In addition, in the absence of classical mitogens, BH also stimulated B- and T-cells with stimulatory indices of 0.247 ± 0.03 and 0.34 ± 0.04, respectively. In the absence of LPS, maximum NBT uptake (fmol of formazan per phagocyte) by neutrophils was achieved at 0.2% BH (1.53 ± 0.23 compared to 1.29...

Survey of aflatoxin B1 and ochratoxin A in commercial green coffee beans by high‐performance liquid chromatography linked with immunoaffinity chromatography

Abstract: The natural occurrence of aflatoxin B1 (AFB1) and ochratoxin A (OTA) has been surveyed in 47 samples of commercial green coffee beans. Samples were collected in Nagoya City, Japan, during 1988–93. Using a combination of immunoaffinity chromatography and high‐performance liquid chromatography analysis, AFB1 and OTA were quantified with detection limits of 2 ng kg‐1 and 0.1 μg kg‐1 respectively. Positive rates and levels of AFB1and OTA were 32% and 2.0–32.9 ng kg‐1, and 30% and 0.1–17.4 μg kg‐1 respectively. The samples contaminated with AFB1 or OTA were mainly imported from African and Asian countries, and several samples were found to be positive for these two mycotoxins for the first time in Japan, although their levels were low.

Effect of heat treatment on the antigenicity of bovine α-lactalbumin and β-lactoglobulin in whey protein isolate

Abstract: The effect of heat treatment on the antigenicity of α-lactalbumin (α-LA) and β-lactoglobulin (β-LG) in whey protein isolate (WPI) was investigated at temperatures in the range of 50–120°C for times from 0.5 to 30 min. The antigenicity was determined by an indirect competitive enzyme-linked immunosorbent assay. The heat denaturation of α-LA and β-LG was revealed by SDS-PAGE electrophoresis. The antigenicity of α-LA and β-LG increased with increasing temperature from 50 to 90°C and the highest antigenicity of α-LA and β-LG was detected at 90°C. However, above 90°C the antigenicity of both proteins showed a remarkable decrease. When treated at 120°C for 20 min, the antigenicity of α-LA decreased below the initial value of the untreated sample. The antigenicity of α-LA and β-LG in WPI can be decreased at certain heat temperature and time.

Improved enzyme immunoassay for the analysis of s‐triazines in water samples

Abstract: A highly sensitive enzyme immunoassay is described for the detection of the s‐triazines, propazine and atrazine. Polyclonal antibodies were raised in rabbits by immunization with an atrazine‐BSA conjugate containing 35 atrazine residues per molecule. A microtitration plate ELISA was optimized with a peroxidase‐atrazine tracer. The middle of the test (50% (B/B0) was found at 10 ng/l propazine and 20 ng/l atrazine, i.e. well below the maximum concentration permitted by the EC guidelines for drinking water. Detection limits for atrazine of about 1 ng/l and for propazine of around 0·1 ng/l could be reached. The assay did not require concentration or clean‐up steps for drinking or ground water samples. Validation experiments showed a good accuracy and precision. Since propazine is not used in most European countries, this test can be applied to obtain fairly accurate results for atrazine concentrations in environmental water samples from these regions.

Studying the impact of nutritional immunology underlying the modulation of immune responses by nutritional compounds – a review

Abstract: Nutritional immunology perception is a condition in which an altered supply of nutrients modifies immune response, and it has become linked intimately with endeavors to improve the clinical patient...