Showing papers in "Food Quality and Safety in 2020"

Impact of COVID-19 on the food supply chain

Abstract: A pandemic is not a new event encountered in the history of humanity because mankind has faced various pandemics in history The common point of pandemics is their serious negative effects on the global economy Considering the food supply chain, one of the most important sectors of the economy, it has been seen that COVID-19 has an impact on the whole process from the field to the consumer In the light of recent challenges in food supply chain, there is now considerable concern about food production, processing, distribution, and demand COVID-19 resulted in the movement restrictions of workers, changes in demand of consumers, closure of food production facilities, restricted food trade policies, and financial pressures in food supply chain Therefore, governments should facilitate the movement of workers and agri-food products In addition, small farmers or vulnerable people should be supported financially Facilities should change the working conditions and maintain the health and safety of employees by altering safety measures Food protectionist policies should be avoided to prevent an increase in food prices In conclusion, each country must realize the severity of the situation and sometimes should tighten or loosen the measures according to the spread of the pandemic The supply chain also should be flexible enough to respond to the challenges in the food supply chain The purpose of this review is to evaluate the impact of COVID-19 on the agriculture and food sector and to summarize the recommendations required to reduce and control the effect of the pandemic

Impacts of COVID-19 on Food Supply Chain

Abstract: A pandemic is not a new event encountered in the history of humanity, because mankind has faced various pandemics in history The common point of pandemics is their serious negative effects on the global economy Considering the food supply chain, one of the most important sectors of the economy, it has seen that COVID-19 has an impact on the whole process from the field to the consumer In the light of recent challenges in food supply chain, there is now considerable concern about the food production, processing, distribution, and demand COVID-19 resulted the movement restrictions of workers, changes in demand of consumers, closure of food production facilities, restricted food trade policies and financial pressures in food supply chain Therefore, governments should facilitate the movement of workers and agri-food products In addition, small farmers or vulnerable peoples should be supported financially Facilities should change the working conditions and maintain the health and safety of employees by altering safety measures Food protectionist policies should be avoided to prevent an increase in food prices In conclusion, each country must realize the severity of the situation and sometimes should tighten or loosen the measures according to spreadability of the pandemic The supply chain also should be flexible enough to respond to the challenges in the food supply chain The purpose of this review article is to determine the impact of COVID-19 in the agriculture and food sector and to summarize the recommendations required to reduce and control the effect of the pandemic

Role of ethylene response factors (ERFs) in fruit ripening

Abstract: The ethylene response factors (ERFs) belong to the APETALA2/ethylene response factor (AP2/ERF) superfamily and act downstream of the ethylene signalling pathway to regulate the expression of ethylene responsive genes. In different species, ERFs have been reported to be involved in plant development, flower abscission, fruit ripening, and defense responses. In this review, based on the new progress made by recent studies, we summarize the specific role and mode of action of ERFs in regulating different aspects of ripening in both climacteric and non-climacteric fruits, and provide new insights into the role of ethylene in non-climacteric fruit ripening.

The membrane lipid metabolism in horticultural products suffering chilling injury

Abstract: Horticultural commodities suffer chilling injury following exposure to extremely low temperatures, which results in visible symptoms and considerable quality loss. Therefore, it is of significance to understand the mechanism of this physiological disorder and to develop effective strategies to control it. Chilling stress causes alteration in structure and function of the plasma membrane, which is assumed to be the primary event in response to cold stress. During this process, the membrane lipid metabolism plays a pivotal role in membrane fluidity and stability. In this review, we summarized the possible roles of membrane lipid metabolism in the development of chilling injury, having the potential for developing effective strategies to alleviate chilling injury in horticultural products under refrigerated storage in practice.

Organic farming in India: a vision towards a healthy nation

Abstract: Food quality and safety are the two important factors that have gained ever-increasing attention in general consumers. Conventionally grown foods have immense adverse health effects due to the presence of higher pesticide residue, more nitrate, heavy metals, hormones, antibiotic residue, and also genetically modified organisms. Moreover, conventionally grown foods are less nutritious and contain lesser amounts of protective antioxidants. In the quest for safer food, the demand for organically grown foods has increased during the last decades due to their probable health benefits and food safety concerns. Organic food production is defined as cultivation without the application of chemical fertilizers and synthetic pesticides or genetically modified organisms, growth hormones, and antibiotics. The popularity of organically grown foods is increasing day by day owing to their nutritional and health benefits. Organic farming also protects the environment and has a greater socio-economic impact on a nation. India is a country that is bestowed with indigenous skills and potentiality for growth in organic agriculture. Although India was far behind in the adoption of organic farming due to several reasons, presently it has achieved rapid growth in organic agriculture and now becomes one of the largest organic producers in the world. Therefore, organic farming has a great impact on the health of a nation like India by ensuring sustainable development.

In vitro screening of probiotic properties of Lactobacillus plantarum isolated from fermented milk product

Abstract: The screening of traditional fermented products is essential for the assessment of safety, security, and further development of functional foods for the well-being of human health. The aim of the present study was to isolate and identify bacteria from fermented raw milk samples that exhibit health benefits upon consumption. In order to confirm the isolates as probiotics, several in vitro assays were conducted to assess the probiotic properties of isolated bacteria. The initial screening includes tolerance to acid, bile, pancreatin, and NaCl. The cell surface properties demonstrate their interaction with mucosal epithelium, which includes hydrophobicity and auto-aggregation assay. Safety assessment was done by performing haemolytic test and antibiotic susceptibility test. The antagonistic activity of probiotic strain was further evaluated against some pathogenic bacteria. Lactobacillus plantarum (L. plantarum) isolated from fermented raw milk was preliminarily identified by biochemical tests and further confirmed using 16S rRNA identification. The isolate designated as L. plantarum strain GCC_19M1 demonstrated significant tolerance to low pH, 0.3% bile, 0.5% pancreatin, and 5% NaCl. In the presence of simulated gastric juice (at pH 3), the isolate exhibited a survival rate of 93.48–96.97%. Furthermore, the development of ecological niches in the human gut and their successful accumulation have been revealed by auto-aggregation and hydrophobicity properties. Absence of haemolytic activity ensures the non-virulent nature of the strain. Lactobacillus plantarum strain GCC_19M1 showed susceptibility towards gentamicin, tetracycline, kanamycin, meropenem, and ceftriaxone and exhibited an antagonistic effect on pathogenic bacteria. The obtained results conveyed that L. plantarum strain GCC_19M1 has strong probiotic potential, and its presence in the fermented raw milk products may serve as a potent functional probiotic food.

Germination: an alternative source to promote phytonutrients in edible seeds

Abstract: Consumption of less phytonutrient foods has shown to cause different chronic diseases, despite over 50,000 edible plant breed available in various countries around the globe. These edible plants consist of seeds that can be consumed which possessed high health benefits. Moreover, nutritive values such as phytochemicals of edible seeds increased after germination. Therefore, germination has been reported to enhance various bioactive compounds such as γ-amino butyric acid, polyphenols, and vitamins which lead to greater bioactivity such as anti-diabetic, anti-bacteria, and anti-cancer effects when these seeds are consumed. Consequently, germination can be regarded as a cheap and effective way to enhance the nutritional value of edible seeds.

Chemical characterization, release, and bioactivity of Eucalyptus camaldulensis polyphenols from freeze-dried sodium alginate and sodium carboxymethyl cellulose matrix

Abstract: Crude ethanolic extract of Eucalyptus camaldulensis was encapsulated with sodium alginate–sodium carboxymethyl cellulose (CMC) using freeze-drying techniques. The microcapsules were characterized for particle size, morphology, physicochemical parameters, and micromeritics properties. Antioxidant and antimicrobial activities of the microcapsules were also demonstrated. Results revealed an irregular-shaped microparticles with a mean diameter ranging from 6.7 to 26.6 µm. Zeta potential and polydispersity index ranged from −17.01 to 2.23 mV and 0.34 to 0.49, respectively. Percentage yield ranged between 70.4 and 81.5 per cent whereas encapsulation efficiency ranged between 74.2 ± 0.011 and 82.43 ± 0.77 per cent. Swelling index and solubility varied inversely with extract concentration, with a range of 54.4%–84.0% and 18.8%–22.2%, respectively. Antioxidant activities varied directly with the concentration of the extract. Minimum inhibitory and minimum bactericidal concentrations of the microcapsules against Gram-positive foodborne pathogens ranged from 0.19 to 3.12 and 0.19–12.25 mg/ml, respectively. The Higuchi model indicated a time-dependent, delayed, and regulated release of polyphenols at 37°C. The results suggested that alginate–CMC possessed good encapsulant properties that preserved the bioactive extract, thus might be employed for application of natural products in food systems.

Recent progress of the effect of environmental factors on Aspergillus flavus growth and aflatoxins production on foods

Abstract: The contamination of Aspergillus flavus and subsequent aflatoxins (AFs) has been considered as one of the most serious food safety problems due to their acute and chronic adverse effects on humans and animals. This review collects the available information from recent years on the effect of the major environmental factors such as water activity (aw), temperature, CO2, and pH on the fungal growth, the expression of AFs-related genes, and AFs production by A. flavus on foods. In particular, the relationship between the relative expression of key regulatory (aflR and aflS) and structural genes (aflD, aflO, aflQ, etc.) and AFs production under different environmental conditions are collected and discussed. The information collected in this review can be used to design control strategies of A. flavus and AFs contamination in practical applications, primarily during storage and processing. These data suggest that integrating various post-harvest methods with synergistic functions may be more efficient for the control of A. flavus growth and AFs production, although the individual environmental factors alone have an impact.

Safety and quality of bacterially fermented functional foods and beverages: a mini review

Abstract: Bacteria have been employed widely in the food and beverage industry, with evolving dimensions in recent years. Proteases derived from lactic acid bacteria (LAB) are useful in the production of fermented functional beverages and are of particular use in conditioning their shelf life, nutritional content, flavour, and texture quality, thus making fermented foods and beverages functional and therapeutic. This review focuses on bacteria, especially protease-producing LAB used in food processing, and their usefulness in the production of functional foods and beverages. A case study of oat beverages was briefly explored due to its popularity. The safety and quality importance of the food products were also considered with a few recommendations.

Research advance in regulation of fruit quality characteristics by microRNAs

Abstract: MicroRNAs (miRNAs) are short (19–24 nucleotides in length) noncoding RNAs that have a profound effect on gene expression. By completely or almost perfectly base-pairing with their individual target mRNAs they cause mRNA cleavage or repression of translation. As important regulators, miRNAs plays an important role in the regulation of fruit quality. Extensive studies have been reported in fruits, however current studies are mostly focused on the identification of miRNAs and the prediction and validation of target genes. This review summarizes research progress on the role of miRNAs in regulating fruit ripening and senescence and quality characteristics, such as coloration, flavor metabolism, and texture for providing information for future research.

Black goji berry (Lycium ruthenicum) tea has higher phytochemical contents and in vitro antioxidant properties than red goji berry (Lycium barbarum) tea

Abstract: Goji berry tea, a traditional herbal tea, is the main ate mode of goji berry in Asia, yet few studies in comparison with red goji berry tea and black goji berry tea are carried out. This study investigated the effects of water temperature and soak time on the colour, phytochemicals, and the antioxidant capacity [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and the ferric-reducing antioxidant power (FRAP)] of two goji berry tea. A comparison of the bioactive compounds and antioxidant activities between black and red goji berry tea was conducted. Results showed that both red and black goji berry tea were rich in phytochemicals, giving high antioxidant ability. The levels of bioactive compounds and the antioxidant activity of the two goji berry tea increased as the increases in soak temperature and time. Black goji berry tea had higher phytochemicals and antioxidant property than those of red goji berry tea. Infused at 100° water for the same time, the levels of total polysaccharides (150 mg/100 ml), total polyphenols (238 mg/ml), and antioxidant capacity (550 μmol/100 ml) of black goji berry tea were 3.5, 2, and 5 times higher, respectively, in comparison with red goji berry tea. The results of this study demonstrate that hot drink of goji berry in China is a good habit and black goji berry tea may be a better choice.

The impact of gamma irradiation and storage on the physicochemical properties of tomato fruits in Ghana

Abstract: Tomato is a popular fruit that makes significant contributions to human nutrition for its content of sugars, acids, vitamins, minerals, lycopene, and other constituents. The fruit, however, has a short shelf life due to its climacteric nature. In view of this, an experiment was conducted to determine the effect of postharvest treatment on the physicochemical properties of fresh tomato fruits. Freshly harvested tomato fruits were subjected to 0, 1, 2, 3, and 4 kGy gamma radiation and stored at 10 ± 1°C and 28 ± 1°C. Parameters analysed during the study include pH, total titratable acidity, weight loss, total solids, and moisture content of the sample. At both storage temperatures, results of the analyses were in the range of 2.80%–38.67% for weight loss, 0.23%–0.51% for total titratable acidity, 3.5%–5.0% for total soluble solids, 94.43%–96.53% for moisture content, and pH was generally low in the samples stored at 10 ± 1°C. Generally, gamma irradiation had an effect on the total soluble solids, total titratable acids, pH values, and moisture content and physiological weight loss at both storage temperatures. From the study, storing Burkina variety at a low temperature preserves the tomato fruits better than storing them at ambient temperature.

Influences of 1-methylcyclopropene-containing papers on the metabolisms of membrane lipids in Anxi persimmons during storage

Abstract: The aim of this work was to analyse the effects of 1-methylcyclopropene (1-MCP) treatment on the metabolisms of membrane lipids in postharvest Anxi persimmons during storage. Anxi persimmon (Diospyros kaki L. f. cv. Anxi) fruits were treated by paper containing 1-MCP with a concentration of 1.35 μl/l. The cellular membrane permeability was analysed by the electric conductivity meter. The activities of lipoxygenase (LOX), phospholipase (PLD) and lipase were determined by spectrophotometry. The component and relative amounts of membrane fatty acids were determined using gas chromatograph (GC). The 1-MCP-treated Anxi persimmons manifested a lower electrolyte leakage rate, lower LOX, PLD and lipase activities, higher levels of unsaturated fatty acids (USFAs), higher ratio of USFAs to saturated fatty acids (SFAs) (U/S), higher index of USFAs (IUFA), but lower levels of SFAs. The degradation and the metabolisms of membrane lipids could be suppressed by 1-MCP treatment, which might be accountable for the delaying softening of postharvest Anxi persimmons during storage.

Evaluation and optimization of air-based precooling for higher postharvest quality: literature review and interdisciplinary perspective

Abstract: Precooling is of significant importance for postharvest fruits and vegetables to control the quality degradation and prolong the shelf-life. Current precooling methods include room cooling, forced-air cooling, hydrocooling, vacuum cooling, contact or package icing, and cryogenic cooling, all of which have their advantages and disadvantages. The first two methods with the cooling medium of air are extensively used because of the wide applicable range of fruits and vegetables. Numerous studies have been devoted to cope with the drawbacks of these two air-based precooling methods with various evaluation criteria and optimization methods. A systematic literature review on these studies is firstly conducted with respect to experimental and numerical investigations respectively for the two methods. The main contributions from the previous studies are also summarized respectively with the research objectives and performance metrics. The literature review indicates that the current performance evaluation is limited to apparent parameters and the optimal design is only proposed based on the performance evaluation and comparison. Furthermore, with inspiration from the research in other domains, a scheme of advanced evaluation and optimization for air-based precooling methods is proposed with thermodynamic evaluation metrics and constructal optimization methods from the interdisciplinary perspective.

Nutritional evaluation of whole soybean curd made from different soybean materials based on amino acid profiles

Abstract: Amino acid profiles of whole soybean curds (WSCs) and conventional soybean curds (CSCs) were comparatively studied to 1., evaluate their nutritional value and 2., study the suitable soybean material to fabricate WSCs.Nine types of soybean materials were selected to make WSCs and CSCs. Protein digestibility-corrected amino acid score (PDCAAS) and principal component analysis were selected to perform the comparative analysis of amino acid profiles of the products.Total contents of amino acids in WSCs and CSCs were 41.24–54.87 mg/g and 27.77–36.82 mg/g. Content ranges of essential amino acids were 13.59–19.38 mg/g and 8.46–11.76 mg/g for WSCs and CSCs. The essential amino acid pattern of WSCs was basically close to amino acid pattern spectrum requirements except valine and sulfur amino acids. As a limiting amino acid, methionine represented less than 0.4 for PDCAAS in all soybean curds except WSCs made from relay-cropping Nandou12 and Nandou25.The overall quality of amino acids in WSCs was higher than that in CSCs, indicating that WSCs indeed exhibited higher nutritional value than CSCs from the viewpoint of amino acid profile. WSCs with the high nutritional value could be made using Nandou25 and relay-cropping Nandou12 as materials.

CRISPR/Cas9-mediated gene-editing technology in fruit quality improvement

Abstract: Fruits are an essential part of a healthy, balanced diet and it is particularly important for fibre, essential vitamins, and trace elements. Improvement in the quality of fruit and elongation of shelf life are crucial goals for researchers. However, traditional techniques have some drawbacks, such as long period, low efficiency, and difficulty in the modification of target genes, which limit the progress of the study. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technique was developed and has become the most popular gene-editing technology with high efficiency, simplicity, and low cost. CRISPR/Cas9 technique is widely accepted to analyse gene function and complete genetic modification. This review introduces the latest progress of CRISPR/Cas9 technology in fruit quality improvement. For example, CRISPR/Cas9-mediated targeted mutagenesis of RIPENING INHIBITOR gene (RIN), Lycopene desaturase (PDS), Pectate lyases (PL), SlMYB12, and CLAVATA3 (CLV3) can affect fruit ripening, fruit bioactive compounds, fruit texture, fruit colouration, and fruit size. CRISPR/Cas9-mediated mutagenesis has become an efficient method to modify target genes and improve fruit quality.

Advance in yellowing mechanism and the regulation technology of post-harvested broccoli

Abstract: Yellowing is one of the main problems of quality deterioration in the storage, transportation, and sales of post-harvested broccoli, which seriously affects the commodity value of broccoli. Therefore, it is of significance to understand the mechanism of the process and develop effective regulation technology. In this review, we expounded the changes in the appearance of the flower ball, bud morphology, and calyx cell structure, as well as endogenous pigment metabolism, accompanying the yellowing process of broccoli. In addition, recent research on the molecular mechanism of yellowing was summarized from the aspects of transcriptome analysis and transcription regulation. Finally, the progress on the control technology of broccoli yellowing was reviewed.

Impact of ventilation design on the precooling effectiveness of horticultural produce—a review

Abstract: Optimizing the ventilation design of packaging system is of crucial importance for improving the efficiency of the forced-air precooling process to maintain the quality of horticultural produce and extend the shelf life in food cold chain. Many efforts had been devoted to the study about the impact of ventilation design on airflow and temperature distribution inside ventilated packages. This paper reviews relevant research methods, commonly used quantities for the measurement of precooling effectiveness, attractive design parameters, and their impact on precooling effectiveness. These allow us to know exactly the characteristic and deficiency of each research method, identify dominant design parameters, and seek a promising way for the future improvement of the ventilated packaging system.

Selection of nitrite-degrading and biogenic amine-degrading strains and its involved genes

Abstract: Accumulation of nitrite and biogenic amines (BAs) in fermented meat products is a matter of public health concern. The study aimed to screen nitrite-degrading and BA-degrading strains from sour porridges and sausages and bacon products in China. After screening out 12 strains, the degradation of nitrite, the degradation of BAs, the activities of nitrite-reducing enzymes, and the detection of genes involved in the BAs were assessed by spectrophotometry method with hydrochloric acid naphthalene ethylenediamine, high-performance liquid chromatography, GENMED kit, and polymerase chain reaction, respectively. Pediococcus pentosaceus labelled M SZ1 2 and M GC 2, Lactobacillus plantarum labelled M SZ2 2, and Staphylococcus xylosus labelled Y CC 3 were selected. The activity of nitrite-reducing enzyme in M SZ2 2 was 2.663 units/mg. The degradation rate of total BAs of M SZ2 2 was 93.24%. The degradation rates of nitrite and BAs of M SZ1 2 were 86.49% and 37.87%, respectively. The activity of nitrite-reducing enzyme in M SZ1 2 was up to 1.962 units/mg. M GC 2 showed higher degradation rates of nitrite (89.19%) and Y CC 3 showed higher degradation rates of BAs (36.16%). The genes encoding the multicopper oxidases (suf I/D2EK17) were detected in the four strains, which also did not contain BAs (histidine decarboxylase (hdc), tyrosine decarboxylase (tdc), ornithine decarboxylase (odc), lysine decarboxylase (ldc)) formation encoding genes. These four strains (M SZ1 2, M GC 2, M SZ2 2, and Y CC 3) are promising candidates to use as starter cultures for nitrite and BAs in fermented sausages.

Effects of nitric oxide treatment on flavour compounds and antioxidant enzyme activities of button mushroom (Agaricus bisporus) during storage

Abstract: Button mushroom (Agaricus bisporus) is sold well for its unique flavour and nutritional benefits. However, the mushroom flavour deteriorates quickly during storage because of its delicate structure and high moisture. In this study, the effects of nitric oxide (NO) application on flavour compounds and antioxidant enzyme activities of stored button mushrooms were investigated. The button mushrooms were immersed in the NO donor sodium nitroprusside (15 μmol/L) for 3 min and then stored under the condition of 4 °C, 90% relative humidity for 12 days. Results showed that the treated mushrooms have reduced weight loss rate, uniform white colour, and higher firmness during storage. Compared to the control, the ketones, alcohols, esters, and aldehydes in the NO-treated button mushroom increased sharply at 3 days of storage and then showed a continuing decline trend, except ester compounds which reached the peak value at 6 days of storage. In addition, NO treatment increased the total phenolics and catalase activity and inhibited the polyphenol oxidase activity in the stored button mushroom. These results indicated that NO treatment is an alternative storage technology to enhance antioxidant capacity and maintain flavour and consumer acceptance of stored button mushroom.

Application of electronic nose and GC–MS for detection of strawberries with vibrational damage

Abstract: This study evaluated the potential of using electronic nose (e-nose) technology to non-destructively detect strawberry fruits with vibrational damage based on their volatile substances (VOCs). Four groups of strawberries with different durations of vibrations (0, 0.5, 1, and 2 h) were prepared, and their e-nose signals were collected at 0, 1, 2, and 3 days after vibration treatment. The results showed that when the samples from all four sampling days during storage were used for modelling, both the levels of vibrational damage and the day after the damage happened were accurately predicted. The best models had residual prediction deviation values of 2.984 and 5.478. The discrimination models for damaged strawberries also obtained good classification results, with an average correct answer rate of calibration and prediction of 99.24%. When the samples from each sampling day or vibration time were used for modelling, better results were obtained, but these models were not suitable for an actual situation. The gas chromatography–mass spectrophotometry results showed that the VOCs of the strawberries varied after experiencing vibrations, which was the basis for e-nose detection. The changes in VOCs released by other forces should be studied in the future. The above results showed the potential use of e-nose technology to detect strawberries that have suffered vibrational damage.

Comparative in vitro Study of the biological activity and chemical composition extracts of Helicteres isora L. obtained by water and subcritical water extraction

Abstract: Subcritical water extraction technique is considered as an environmentally extraction technique. The aim of this study was to compare the different characteristics of water extract and subcritical water extract of Helicteres isora L. Water extraction was performed under the following conditions: 25°C, 24 h, and solid-to-water ratio 1:30. Subcritical water extract was carried out under specific conditions (pressure = 10 bar, temperature = 160°C, solid-to-water ratio = 1: 30, time = 30 min). Chemical composition analysis was performed using GC–Mass chromatography. Anti-biofilm activity in the terms of anti-attach and removal of biofilm were assessed using the ELISA reader method and reading absorbance at 570 nm. Anti-microbial activity against Bacillus cereus, Staphylococcus aureus, Staphylococcus saprophyticus, and Bacillus subtilis was investigated by measurement of inhibitory zone diameter. Anti-enzymatic and antioxidant properties were also assessed. The results of GC–Mass analysis showed some components extracted in subcritical method which were absent in water extract such as octadecanoic acid, hexadecanoic acid, and berberin. Antioxidant activity of the two tested extracts revealed that subcritical water extract had more antioxidant capacity than water extract (P ≤ 0.05). The two tested extracts exhibited anti-enzymatic activity against polyphenol oxidase enzyme with better performance of subcritical water extract. Anti-biofilm activity of the two extracts implies that, in the case of preventing biofilm formation, both extracts had similar efficiency but in the removal of biofilm, subcritical water extract showed better performance. Both extracts had anti-microbial activity against B. cereus, S. aureus, S. saprophyticus, and B. subtilis with better performance of subcritical water extract. Anti-enzymatic assay also showed similar results. Subcritical water extract of H. isora showed more antioxidant activity as well as anti-biofilm, anti-bacterial, and anti-enzymatic activity rather than ordinary water extract.

Metabolomics of ginger based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry technology

Abstract: Dried ginger and ginger are the same type of medicine and food. The differential components of ginger and dried ginger, dried ginger and ginger charcoal were investigated. The experimental materials were divided into three sample groups: the ginger group, dried ginger group, and ginger charcoal group. The ginger group, dried ginger group, and ginger charcoal group were qualitatively analyzed by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The data were processed by Marker View Software. Principal component analysis and orthogonal partial least-square discriminant analysis were performed with SIMCA 13.0 Software. The differential components of the ginger and dried ginger groups as well as the dried ginger and ginger charcoal groups with a variable importance in the projection > 2 (P < 0.05) were identified with PeakView 1.2 Software. Ten differential components, including 6-gingerol, 8-gingerol, and 10-gingerol, were identified between the ginger group and dried ginger group; 13 differential components, including 6-shogaol, 10-gingerol, and zingiberone, were identified between the dried ginger group and ginger charcoal group. The main differential components between the ginger and dried ginger groups and the dried ginger and ginger charcoal groups were gingerols and diphenylheptanes. Based on metabolomics analysis of the chemical composition of ginger’s medicinal materials, effects, and other related factors, it is recommended that 6-gingerol, 6-shogaol, and zingiberone should be used as indicative components for the respective quality evaluation of ginger, dried ginger and ginger charcoal. The results of this study may provide a basis for the reasonable quality evaluation of ginger medicinal materials.

Discrimination of Chuanminshen violaceum Sheh et Shen from different regions based on fatty acid profiles of roots and leaves

Abstract: The purpose of this paper was to construct a reliable methodology to discriminate the geographical origins of Chuanminshen violaceum Sheh et Shan planted in different regions in Sichuan, China. Fatty acid profiles of roots and leaves of C. violaceum planted in various regions of Sichuan Province in China, namely Guangyuan (GY), Langzhong (LZ), Jintang (JT), Bazhong (BZ), and Shuangling (SL), were determined using GC-MS followed by multivariate statistical analyses, including orthogonal partial least-squares discriminant analysis and hierarchical clustering analysis. Leaves of C. violaceum showed the highest contents of hexadecatrienoic acid (3.21 g/kg), linoleic acid (6.62 g/kg), and α-linolenic acid (7.24 g/kg), which were all higher than those contained in roots. Chuanminshen violaceum samples collected from LZ, JT, and GY could be clearly distinguished based on fatty acid profiles of leaves and those collected from LZ, GY, and BZ could be clearly distinguished based on fatty acid profiles of roots. Chemometric method is used as a potential approach for analyses of fatty acid profiles of roots and leaves to control the quality of C. violaceum and their powered products.

Assessing the supercooling of fresh-cut onions at −5°C using electrical impedance analysis

Abstract: We supercooled fresh-cut onion at −5°C for 12 h. After supercooling, the electric impedance properties of the samples were evaluated by electrical impedance spectroscopy over the frequency range of 42 Hz − 5 MHz. The time-temperature profiles of samples indicated that the freezing point and supercooling point were −2.3°C ± 0.7°C and −6.9°C ± 1.0°C, respectively. The results indicated that 34 of the 36 supercooled samples exhibited a definite circular arc in the Cole-Cole plot, which suggested that the cell membrane remained intact during supercooling. In the other two samples which did not exhibit a definite circular arc, the cell membrane had sustained serious damage during supercooling. Furthermore, there was large difference in drip loss percentage between supercooled samples exhibited a definite circular arc in the Cole-Cole plot and samples not exhibiting a definite circular arc. Our results suggest that fresh-cut onions can be supercooled at −5°C.

A review on the consumption of vended fruits: microbial assessment, risk, and its control

Abstract: Fruits are very beneficial to health and their consumption has been recommended in daily diets. However, when fresh fruits are harvested and processed into sliced ready-to-eat or vended forms for sale, hygienic procedures are neglected. Thus, they are easily infested by pathogens. In addition, uneducated vendors who sell these fruits often display or hawk them on the streets in contaminated containers, utensils, or dirty environments. This poses a great threat to the health of consumers. In the light of this realization, some microbial assessments have been carried out to ascertain the safety of these vended fruits, thus making it a necessity to exploit the outcomes of some of these microbial assessments on vended fruits in order to sensitize the consumers on the effect of their consumption and mitigate risk by improving their quality. This will assist to lower certain health concerns, an outbreak of diseases, and death.

Carotenoid metabolism in mitochondrial function

Abstract: Mitochondria are highly dynamic organelles that are found in most eukaryotic organisms. It is broadly accepted that mitochondria originally evolved from prokaryotic bacteria, e.g. proteobacteria. The mitochondrion has its independent genome that encodes 37 genes, including 13 genes for oxidative phosphorylation. Accumulative evidence demonstrates that mitochondria are not only the powerhouse of the cells by supplying adenosine triphosphate, but also exert roles as signalling organelles in the cell fate and function. Numerous factors can affect mitochondria structurally and functionally. Carotenoids are a large group of fat-soluble pigments commonly found in our diets. Recently, much attention has been paid in carotenoids as dietary bioactives in mitochondrial structure and function in human health and disease, though the mechanistic research is limited. Here, we update the recent progress in mitochondrial functioning as signalling organelles in human health and disease, summarize the potential roles of carotenoids in regulation of mitochondrial redox homeostasis, biogenesis, and mitophagy, and discuss the possible approaches for future research in carotenoid regulation of mitochondrial function.

Trigonella foenum-graecum seeds extract plays a beneficial role on brain antioxidant and oxidative status in alloxan-induced Wistar rats

Abstract: Trigonella foenum-graecum (TriFG) exhibits increased scavenger enzymatic activities and reduces the production of reactive oxygen species in diabetic rats.The present study was aimed to investigate the effect of TriFG on lipid peroxidation levels and antioxidant status in brain tissue of rats exposed to alloxan.Healthy male rats (180 ± 10 g) were allocated into five groups. Animals in group 1 maintained on normal tap water served as controls and rats in groups 2, 3, 4, and 5 were treated as experimental groups. Rats in group 2 were intraperitoneally injected with alloxan (120 mg/kg BW) and treated as diabetic rats, whereas rats in groups 3 and 4 were maintained on same experimental regimen as that of rats in groups 1 and 2, respectively, and in addition, they were orally gavaged with herbal extracts of TriFG (0.25 g/kg BW). Diabetic rats treated with glibenclamide in group 5 were used as positive controls.Significant (P < 0.001) increase in the antioxidant enzymes with a significant (P < 0.001) decrease in the lipid peroxidation levels were observed in the brain tissue of diabetic rats treated with TriFG extract as compared to diabetic and glibenclamide-treated rats. No significant changes were observed in pro- and antioxidant levels in brain tissue of rats treated with TriFG extract alone when compared to normal rats. In diabetic rats, brain mitochondrial and cytosolic enzymes like succinate dehydrogenase, glutamate dehydrogenase, and glucose-6-phosphate dehydrogenase activity levels were significantly (P < 0.05) decreased with reversely increased was observed in lactate dehydrogenase activity (P < 0.05).The findings of the present study suggested that TriFG, through its antioxidant properties, protects brain tissue by mitigating oxidative stress induced by alloxan-exposed rats. TriFG extract significantly increased the antioxidant and oxidative properties in diabetic rats when compared with the control group rats.

Retraction: From farm to fork: global surveillance trends of animal-food-human antimicrobial resistance

Abstract: Global surveillance measures keep trending with antimicrobial resistance (AMR) from farm to the final consumer. It is intended to review these trends within the past few decades. This short communication showcases AMR surveillance basics, methods, processes, technicalities and milestones within recent decades in relationship to AMR emergence from farm to fork. Antibiotics and antimicrobial agents contribute to AMR dynamics. Passive and active AMR surveillance strategies continuously render data-driven robust systems for monitoring resistance levels and thereof changes across all geographical regions.