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JournalISSN: 0378-1119

Gene 

Elsevier BV
About: Gene is an academic journal published by Elsevier BV. The journal publishes majorly in the area(s): Gene & Complementary DNA. It has an ISSN identifier of 0378-1119. Over the lifetime, 23248 publications have been published receiving 948752 citations. The journal is also known as: genes.


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Journal ArticleDOI
01 Jan 1985-Gene
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.

14,954 citations

Journal ArticleDOI
Steffan N. Ho1, Henry D. Hunt1, R. M. Horton1, Jeffrey K. Pullen1, Larry R. Pease1 
15 Apr 1989-Gene
TL;DR: In this paper, complementary oligodeoxyribonucleotide (oligo) primers and the polymerase chain reaction are used to generate two DNA fragments having overlapping ends, and these fragments are combined in a subsequent 'fusion' reaction in which the overlapping ends anneal, allowing the 3' overlap of each strand to serve as a primer for the three' extension of the complementary strand.

7,528 citations

Journal ArticleDOI
15 Jul 1988-Gene
TL;DR: Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.

6,003 citations

Journal ArticleDOI
01 Oct 1982-Gene
TL;DR: A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed and a kanamycin-resistance marker has been inserted into the center of the symmetrical MCS7 to yield a restriction-site-mobilizing element (RSM).

5,719 citations

Journal ArticleDOI
01 Nov 1977-Gene
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).

5,235 citations

Performance
Metrics
No. of papers from the Journal in previous years
YearPapers
202328
2022104
2021576
2020662
2019835
20181,020