Showing papers in "Gene in 1988"

TL;DR: Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.

TL;DR: An approach for performing multiple alignments of large numbers of amino acid or nucleotide sequences is described, based on first deriving a phylogenetic tree from a matrix of all pairwise sequence similarity scores obtained using a fast pairwise alignment algorithm.

TL;DR: The production of new alleles of the LEU2, URA3 and TRP1 genes of Saccharomyces cerevisiae by in vitro mutagenesis is described and a unique series of yeast-Escherichia coli shuttle vectors derived from the plasmid pUC19 are constructed.

TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.

TL;DR: Improved broad-host-range plasmid vectors were constructed based on existing plasmids RSF1010 and RK404 and have several additional cloning sites and improved antibiotic-resistance genes which facilitate subcloning and mobilization into various Gram-negative bacteria.

TL;DR: To ensure complete repression of the hybrid trp/lac promoter during construction and growth in any host strain, the lacIq allele of the lac repressor gene was added to some of the vectors.

TL;DR: Fusion-phage vectors that accept foreign DNA inserts with little effect on phage function are introduced; affinity purification of virions bearing a target determinant from a 10(8)-fold excess of phage not bearing the determinant is described, using minute amounts of antibody.

TL;DR: A simple and convenient phase extraction assay for chloramphenicol (Cm) acetyltransferase (CAT) activity has been developed, based on the enzymatic butyrylation of radiolabelled Cm, which is highly sensitive, and substantially less expensive than all presently available alternatives.

TL;DR: In transient expression experiments using transfected mammalian cells, it is demonstrated that SEAP yields results that are qualitatively and quantitatively similar, at both the mRNA and protein levels, to parallel results obtained using established reporter genes.

TL;DR: Vectors were constructed that allow foreign peptides to be expressed in Escherichia coli as fusion proteins that can be directed to the periplasm by including the leader sequence from the phoA gene on the vector.

TL;DR: A plasmid vector has been constructed that directs the synthesis of high levels of fusions between a target protein and maltose-binding protein in Escherichia coli and a prokaryotic and a eukaryotic protein have been successfully purified by this method.

TL;DR: Nine P-element vectors that can be used to study gene regulation and function in Drosophila are described, designed for use in germline transformation and cell culture transfection assays.

TL;DR: A general method is described to convert virtually any small or medium-sized plasmid into a positive-selection vector and several symmetrical polylinkers and drug-resistance cassettes within the vectors.

TL;DR: Sequence elements conserved among the available set of 66 group I introns are compiled and the possible significance of conserved nucleotides within base-paired duplexes is discussed.

TL;DR: During the construction of these new vectors data were obtained which demonstrated that the pUC and pMTL plasmids contain a previously unreported single base pair difference within the RNA I/RNA II region responsible for a three-fold increase in plasmid copy number.

TL;DR: Comparison of the fungal cellulase structures has led to re-evaluation of hypotheses concerning the localization of the active sites, and all the four T. reesei cellulases share two common, adjacent sequence domains, which apparently can be removed by proteolysis.

TL;DR: Significant homology of the pat gene was found to the bialaphos-resistance gene (bar) of Streptomyces hygroscopicus but variations were detected in the 5'-noncoding region of the two resistance genes which may reflect differences in regulation.

TL;DR: In this article, a new series of vectors, pSU2716, PU2717, SU2718, and SU2719, has been constructed, which contain the P15A replicon, chloramphenicol acetyl transferase (CAT)-coding gene from Tn 9, and the Hae II fragment which carries the multiple cloning site and the lacZ α reporter gene.

TL;DR: Molecular cloning and sequencing of both the alleles of p53 gene revealed a base-pair change in codon 72 causing arginine----proline substitution in the allele with the additional BglII site.

TL;DR: Differences in mRNA stability can contribute to differential expression of genes within polycistronic operons and to modulation of gene expression in response to changes in bacterial growth conditions.

TL;DR: The mdr3 gene codes for a member of a family of membrane proteins, the P-glycoproteins, overproduced in many multi-drug-resistant (MDR) cell lines, and its high homology with mdr1 suggests that it also encodes an efflux pump with broad specificity.

TL;DR: An in vitro selection procedure for oligodeoxynucleotide-directed mutagenesis, which produces mutants at frequencies of greater than 90%, facilitating the identification of mutants directly by nucleotide sequencing is described.

TL;DR: From what can be found in the naturally occurring antisenseRNAs, strategies in designing artificial antisense RNAs for gene expression are proposed, and applications and potential problems discussed.

TL;DR: An industrial Penicillium chrysogenum strain was transformed using two dominant selection markers, namely the bacterial gene for phleomycin resistance fused to a fungal promoter, and the acetamidase (amdS) gene from Aspergillus nidulans, showing Cotransformation was very efficient when using amdS as a selection marker.

TL;DR: The single actin gene from the filamentous fungus Aspergillus nidulans has been isolated and characterized and is predicted to predict a polypeptide containing the N- terminus identifying the gamma-actin isotype.

TL;DR: Two different cellobiohydrolases, CBHI and CBHII, of the filamentous fungus Trichoderma reesei both hydrolyse highly crystalline cellulose, and both of the two recombinant cellulases were able to degrade amorphous cellulose.

TL;DR: A family of 20 Escherichia coli-yeast shuttle vectors, pUN plasmids, containing ARS1 CEN4 and a variety of selectable markers as well as the SUP11 gene which can act as a color marker in the proper background are developed and used for the sectoring-shuffle assay and for gene analysis in general.

TL;DR: The BamHI fragment containing the actIII gene, from the actinorhodin (Act) biosynthetic gene cluster of Streptomyces coelicolor A3(2), was sequenced and high resolution transcript mapping identified the transcription start point at 33 nucleotides upstream of the putative translation start codon.

TL;DR: A novel ribosome-binding site (RBS; phage T7 'gene 10 leader') is described, able to drive the translation of several foreign genes, which dramatically enhanced the translation efficiency of all the genes tested to date, and was particularly effective for foreign genes.

TL;DR: The nucleotide sequence of the protective antigen (PA) gene from Bacillus anthracis and the 5' and 3' flanking sequences were determined and appear to encode a signal peptide having characteristics in common with those of other secreted proteins.