Imaging & Microscopy 

About: Imaging & Microscopy is an academic journal. The journal publishes majorly in the area(s): Microscopy & Microscope. It has an ISSN identifier of 1439-4243. Over the lifetime, 189 publications have been published receiving 1000 citations.

Tomographic Phase Microscopy

Abstract: In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle.

Digital In-line Holographic Microscopy

Abstract: Three-dimensional imaging with micron resolution and simultaneous tracking of many (hundreds) objects (bubbles, beads, algae, bacteria etc.) is now possible in-situ not only with a benchtop microscope, but also remotely in such environments as the deep ocean with a submersible version of the instrument. The instrument is very simple in its hardware requirements and an efficient software package is available for real time reconstruction and image manipulation.

From Macro To Nano

Abstract: The National Institute for Biological Standards and Control (NIBSC) is recognised as a leading authority in the field of biological medicines and medical applications of biotechnology. Our established role includes provision of specialist advice in quality and safety and support in response to public health threats and concerns. The imaging facility at NIBSC operates as a centralised resource. It is necessary to support all aspects of imaging at NIBSC, including: operation of the advanced imaging facilities, training of new users, consultancy on the appropriate imaging solution to the scientific question, advice on selection and maintenance of all microscopes and image capture devices from stereo-dissection microscopes, optimised for stem cell culture, to epifluorescent microscopes. The diversity of biological medicines encountered at NIBSC present unique challenges to the staff of the imaging facility, making our role as a centralised resource both challenging and rewarding.

Huygens Remote Manager

Abstract: Nowadays, deconvolution in cell and tissue imaging has matured into a standard restoration technique that is accessible to large fraction of the microscopy community thanks to steadily improving algorithms. Still, deconvolution is often the ratelimiting step in the analysis of the acquired data, even at today's computer performance. Here, we present the Huygens Remote Manager, an opensource, efficient, multi-user web-based interface for parallel batch deconvolutions.

Correlative 3D microscopy: CLSM and FIB/SEM tomography used to study cellular entry of vaccinia virus

Abstract: The development of new anti-viral strategies requires detailed information on the replication cycle of viruses. In this study we focused on the early steps of viral interaction, the surface dynamics after binding to non-infected cells. We used the more abundant infectious form of the virus, the intracellular mature virus (IMV). IMVs have a dumbbell-shaped core, a single lipid bilayer, and a size of 360 nm in the longest dimension [1]. IMVs have been observed to bind to filopodia, actin-containing, finger-like cell protrusions. Live cell imaging showed that fluorescent virus particles associate with filopodia, and glide along them to the cell body [2]. They also induce the extrusion of large, transient membrane blebs, which upon retraction cause endocytic internalization of the virus [3].