Showing papers in "Immunochemistry in 1965"

TL;DR: By standardizing the technical conditions of the experiment it is possible to use this principle for the immunochemical determination of antigens, and the lower limit of the method was found to correspond to 0·0025 μg of antigen, and to an antigen concentrations of 1·25 μg per ml.

TL;DR: A small but significant difference between the amino acid composition of IgM and IgG antibodies indicated a difference of the primary structure of these antibodies.

TL;DR: Cellulose chemistry and applications as carrier for proteins, polymers, and organic molecules are discussed in detail in this article, where the authors present a collection of applications of cellulose as a carrier.

TL;DR: The capacity of the adsorbent for anti-bovine serum albumin antibody was somewhat lower, but still much higher than that for adsorbents previously reported.

TL;DR: The b 4 and b 5 light chains were not distinguishable by the disc electrophoresis in polyacrylamide gel although each preparation consisted of at least seven discrete electrophoretic light chain components.

TL;DR: The neutralization given by L polypeptide chains from anti-T1 γ-globulin was markedly enhanced by goat anti-papain fragment I seru which had been absorbed with papain fragment III and by fragment III-absorbed anti-fragment I serum.

TL;DR: The results are compatible with the hypothesis that the heterogeneity in S rate of γA-globulin is due to polymerization.

TL;DR: The equilibrium constant for the combination of rabbit anti- p -nitrophenyl antibodies with the dye hapten 4,5-dihydroxy-3-naphthalene disulfonic acid (DHNDS-NP) was determined as 5.8 × 10 5 m −1 from spectrophotometric measurements; the results of equilibrium dialysis experiments were not meaningful because of the strong adsorption of the dyeHapten on the dialysis membrane.

TL;DR: In this paper, a methode de preparation de la β 2 -macroglobuline du serum humain comportant trois etapes: 1 (1) Elimination des lipoproteines a l'aide du sulfate de dextrane, 2 (2) Precipitation des euglobulinine a l 'aide d'une solution d'acide borique, 3 (3) Filtration sur gel de DExtrane

TL;DR: A comparison of the binding by the γG antibody with the binding previously described for the εA antibody shows that the affinity of the latter for the Lac dye is much greater, which may partially explain the heterogeneity of hapten binding in purified antibody.

TL;DR: A transferase hypothesis is proposed to explain the fixation of C′2 on SAV′4 by enzaymatic action of C″1a, suggested that C′1a exposes a reactive group on C′3,0 which can either react with a receptor onC′4 resulting in fixation, or with water, leading to inactivation.

TL;DR: Two crystalline immunoglobulins from the urine of a single patient have been characterized and it is suggested that the κ-chain protein appeared to be a portion of the larger Bence-Jones protein molecule and thus could be a precursor.

TL;DR: In amino acid, fingerprints and carbohydrate analyses no differences have been found between diptheria and tetanus 7 S T -components and fragments, however, these findings do not allow the conclusion, whether the antibody specificity is based on the primary structure or not.

TL;DR: The shape of the anti-C′2 neutralization curve indicates that a single molecule of antibody suffices for inhibition of C′2, and that the C′1a,4,4 molecule can bind several molecules of neutralizing antibody.

TL;DR: It is concluded that in its enzymatic activity, papain does not distinguish between it own antibody and the other γ-globulin molecules and degraded tham indicriminately.

TL;DR: Univalent fragments, fractions I and II, of polyoma virys antibody, obtained by papain digestion, followed by chromatography, were examined in the electron microscope, and the width of fragments so arranged was significantly smaller than that of fragments attached randomly.

TL;DR: The preparation of a benzenearsonic acid cellulose derivative for use in the specific purification and fractionation of antibodies directed against the benzen EARSONic acid determinant eliminated essentially all the anti-benzenearonic acid antibody from antisera.

TL;DR: Rabbit antibodies to carboxypeptidase A (CPA) were characterized by various immunochemical techniques including double diffusion in agar, enzyme inhibition and complement fixation, indicating that whereas some antigenic determinants of the enzyme are available in the zymogen, other antigeniceterminants are masked.

TL;DR: Guinea pigs sensitized with p -azobenzenearsonate conjugates of polytyrosine, poly Lys 51 Tyr 49, poly Glu 52 Tyr 48 and poly Ala 34 Glu 36 Tyr 30 showed delayed cross-reactions with the corresponding hapten-bovine serum albumin conjugacy, in conformity with the “carrier-specificity” known to exist in most delayed hypersensitivity reactions.

TL;DR: The portion of horse anti-VIII, precipitable by BSA-Cello was examined by hapten inhibition and p- nitrophenyl -s- d -cellobioside , cellotriose and cellotetraose found to be equipotent inhibitors of precipitation suggesting a specificity directed against the s- d- cellobiosyl or cellotRIose unit.

TL;DR: It was independently confirmed that indirect precipitation gave a higher estimation of molecules with an a-group specificity than did multiple precipitation.

TL;DR: In comparing several alkyldiamines with the amino groups present on the first and last carbons, it was found that those compounds with three to eight carbons were essentially equally effective, but that the two carbon compound had only about one-half the C′1-reducing ability.

TL;DR: A model for this system, which is based on the assumptions that C′1a combines reversibly with SAC′4 and that the formation of SAC‐1a,4,2a from SAC-4 conforms to enzyme kinetics, accounts for all the observed properties of the reaction.

TL;DR: Injection of guinea pigs via the footpad with protein antigens in water-oil emulsion with added mycobacterial peptidoglycolipid resulted in the production of precipitating antibodies of fast (γg1) and slow (γ2) electrophoretic mobility.

TL;DR: Rabbit antibody reacted completely with hemoglobins A 0, A 1, S 0 and a disubstituted parahydroxymercuribenzoate derivative of A 0 , suggesting that these sulfhydryl groups are in an immunogenically silent region.

TL;DR: It is established that neither tyrosine nor any other iodine-reactive residue is present at the active center of anti-p-azophenyltrimethylammonium antibody and the gradual losses in immunological activity during extensive iodination are the result of nonspecific changes in the tertiary structure.

TL;DR: Structural features of antigenic groupings of oat glucan involved in the cross reaction with Type VIII horese antipneumococcal serum were examined by quantitative hapten inhibition and the cross reactive portion of anti-VIII could be shown to interact as well as terminal non-reducing cellodextrinyl groups.