Showing papers in "Immunochemistry in 1969"

TL;DR: Conjugation of peroxidase, glucose oxidase, tyrosinase and alkaline phosphatase to human immunoglobulin-G, human serum albumin, sheep antibody and rabbit antibody was carried out with glutaraldehyde to characterize the antibodies after immunoelectrophoresis.

TL;DR: Insoluble antigen and antibody derivatives were obtained and were found to be efficient, specific and stable immunoadsorbents, and were used, either in column procedure or in batchwise operation, for the isolation of antigens or antibodies.

TL;DR: Using tetraazotized o -dianisidine, cyanuric chloride or glutaraldehyde, antigens were coupled to erythrocytes and the agglutination reaction was specific and clear cut pictures of positive or negative hemmagglutination were obtained.

TL;DR: Fundamental quantitative relations between the titre and the parameters easily measurable in the test system as well as a description of the experimental procedure for the determintion of the titres of plasma protein antisera are given.

TL;DR: A transplantable BALB/c mouse plasmacytoma, S63, produces an immunoglobulin with antibody activity directed specifically against the C-carbohydrate of the pneumococcus that is of the IgA class and possesses a kappa light chain.

TL;DR: Evidence was presented which indicated the slowest migrating component was responsible for the inhibitory activity of an inhibitor which was capable of interfering with complement mediated hemolysis of antibody sensitized sheep E.

TL;DR: In this article, extracts from human E stromata were found to be capable of causing inhibition of the complement mediated lysis of antibody sensitized sheep red cells, and the inhibition resulted from an increase in the rate of SAbC′la, 4, 2a decay to the SAbc′la 4 state.

TL;DR: There was evidence that modified lysozyme showed decreased antigenicity in rabbits and significant conformational changes accompanied the modification as evaluated by the availability of the disulfide linkages to reduction and the susceptibility of the modified protein to proteolysis.

TL;DR: A reversed version of conventional single radial immunodiffusion was tested on five different antigen-antibody systems and the laws governing the conventional and reversed radial immunODiffusion processes were found to be formally similar.

TL;DR: Reconstitution of hemoglobin from globin and resynthesized heme gave a preparation having conformational parameters and immunochemical behavior identical with those of the native protein.

TL;DR: The rotational relaxatin time of the DNS-HGG conjugate obtained by measuring the fluorescence polarisation was found to be several times lower than calculated on the assumption that the molecule is rigid, which points to the pronounced flexibility of HGG molecule.

TL;DR: The combination of the enzyme with antibodies did not affect its enzymatic activity as measured by K m values and substrate specificity, and the enzyme in complex with antibody exhibited a remarkable stability at this temperature for the periods tested (up to 90 min).

TL;DR: In this article, the authors compared purified type 12 M protein extracted from streptococcal cell walls with alkaline buffers at 37°C and acid-extracted M protein (Lo-M) had a minimum molecular weight of about 20,000.

TL;DR: Evidence is provided for a single kind of light polypeptide chain produced by two different clones of plasma cells in the bone marrow of a patient with an IgG myeloma.

TL;DR: From the results obtained with these standard antigens of varying molecular size and electrophoretic mobility, principles have been derived which should allow the adaptation of this method to the measurement of any soluble protein.

TL;DR: The results suggest that certain structures on both H chains of a given IgG molecule require proper orientation for interaction with the anti-A11 serum, and that the A11 determinant is carried on the heavy ( H ) chain.

TL;DR: Sedimentation velocity measurements on antigen-antibody systems which have been exposed to high concentrations of thiocyanate (SCN), the most effective of these ions, are reported on.

TL;DR: IgA was found to be the predominant immunoglobulin in canine milk, saliva, tears, hepatic bile and intestinal fluid and both exocrine and serum IgA from the dog were readily and completely reduced to subunits of the size of human IgG by treatment with 0·1M mercaptoethanol.

TL;DR: It is concluded that immunoglobulin D, both in its native and aggregated forms, lacks the structural features necessaru for skin binding and complement fixation.

TL;DR: EAC4 prepared with rabbit IgM hemolysins are not suitable for c 1 and Cl titrations on a molecular basis because Cl transfers from site to site even at low ionic strength, and the rate of activation of Cl to Cl is far greater on cells prepared with IgM.

TL;DR: Cell lysis followed first order kinetics during a prolytic phase lasting 1–15 min, depending on C′2 mulitplicity, with first-order release of hemoglobin (Hb) in the control populations.

TL;DR: Chemical, physical and immunological studies indicated that this antibody to the 2,4-DNP grous was a single pure component and essentially 100 per cent active.

TL;DR: The bromoacetyl cellulose immunoadsorbant has been adapted for use as a quantitative micro-method for the measurement of antibody in small amounts of serum, and is especially useful with synthetic polypeptide antigen systems.

TL;DR: Quantitative precipitin tests with purified chicken antibody to the 2,4-dinitrophenyl group indicated a dependence on NaCl concentration for maximum immune precipitation and an Optimum NaCl Molarity, based on an inverse relationship between molarity of NaCl and antibody concentration, resulted in 100% precipitation of antibody and antigen.

TL;DR: The results confirm that the methionine residues (at positions 55 and 131) are not located in antigenic reactive regions in myoglobin, and the myoglobin derivative prepared by recombination of modified apomyoglobin with ferriheme possessed spectral and sedimentation properties that were similar to those of the native protein.

TL;DR: The cross-linked proteins have been found to give a greatly enhanced antibody response compared with the original proteins, and the immunogenic capacity was determined without the use of adjuvants by intravenous injection.

TL;DR: In this article, conditions for the isolation of D-myeloma proteins from serum were studied and the molecular weight of the proteins as determined by the approach to equilibrium technique were 184,000 and 183,000 for the IgD proteins and 161, 000 for IgG.