Showing papers in "Immunopharmacology and Immunotoxicology in 1985"
TL;DR: Only inhibitors of RNA synthesis L-Asparaginase and Actinomycin D reduced non-modulated and IFN modulated NK cell activity.
Abstract: This study investigated the effect of various clinically used chemotherapeutic agents on non-modulated and human alpha Interferon (IFN) modulated natural killer cell (NK) activity. The inhibitors of DNA synthesis, Adriamycin, Cis-Platinum, 5-Fluorouracil and Methotrexate, did not alter NK cell activity at comparable clinically used doses. Similarly, Vincristine and Vinblastine, which are antimitotic agents, did not affect the NK activity. Only inhibitors of RNA synthesis L-Asparaginase and Actinomycin D reduced non-modulated and IFN modulated NK cell activity.
20 citations
TL;DR: It is observed that a short preincubation with theophylline activates human peripheral blood lymphocytes to suppress mitogenic response of autologous cells and this activity was not evident on a T cell subpopulation depleted of theophyLLine-sensitive (T-sens) lymphocytes.
Abstract: It is wellknown that theophylline yields phenotypic changes on suppressor cells. In the present study we investigated the possibility that theophylline could directly induce a suppressor activity on a lymphocyte subpopulation. We observed that a short preincubation (120 min at 37°C) with theophylline (1mM) activates human peripheral blood lymphocytes to suppress mitogenic response of autologous cells. This activity was not evident on a T cell subpopulation depleted of theophylline-sensitive (T-sens) lymphocytes.Theophylline mediated suppressor activity is only present in the Concanavalin A stimulated cultures, thus suggesting a synergism between Concanavalin A and theophylline in the expression of non specific suppression. Moreover we observed that after a 24 hrs preincubation of lymphocytes in complete culture medium there was a complete loss of theophylline-induced suppression. Such a preincubation time also produced a decrease in the theophylline-mediated enhancement of intracellular 3′,5’ cycl...
19 citations
TL;DR: While thymectomy increased the period between skin grafting and rejection, each of the oligopeptides increased the number of splenic T cells and in some extent restored the rejection capacity of thymectomised C57Bl mice, presumably by restoration of thcyic hormone function.
Abstract: The immunostimulatory action of oligopeptides RGH-0205 (Arg-Lys-Asp), RGH-0206 (Arg-Lys-Asp-Val) and TP-5 (4rg-Lys-Asp-Val-Tyr) was measured using the B10LP to C57BL skin graft system and the determination of splenic T cell ratio.While thymectomy increased the period between skin grafting and rejection, each of the oligopeptides increased the number of spenic T cells and in same extent restored the rejection capacity of thymectomised C57B1 mice, presumably by restoration of thymic hormone function.
3 citations
TL;DR: A rapid two step method was used to isolate different lymphokines from large quantities of conditioned media produced by the murine EL-4 cell line, finding that recovery of IL-2 activity was greater than 100% of initial activity.
Abstract: The in vivo administration of lymphokines is a new approach to immuno-therapy with possible applications to the treatment of tumors and infectious processes. These lymphokines are produced in very small quantities and purification of these molecules has proven difficult. A rapid two step method was used to isolate different lymphokines from large quantities of conditioned media produced by the murine EL-4 cell line. Interleukin-2 (IL-2), colony stimulating factor (CSF) and nacrophage activating factor (MAF) are lymphokines with distinct biologic activities and are secreted by the EL-4 cell line. Their isolation involved initial batch purification on trimthylsilyl-controlled pore glass beads followed by reversed phase high pressure liquid chromatography. Recovery of IL-2 activity was >100% of initial activity. The specific activity of the purified IL-2 ranged from 2×106 to 3.6×107 U/mg protein. Colony stimulating factor (CSF) and macrophage activating factor (MAF) were also separated on the chromat...
3 citations
TL;DR: The effects of inactivated streptococci (OK-432) on murine macrophage functions were investigated and the expression of la antigens on peritoneal macrophages was found to be greatly enhanced after in vivo treatment with OK-432.
Abstract: The effects of inactivated streptococci (OK-432) on murine macrophage functions were investigated, in viva treatment of peritoneal macrophages with OK-432 augmented the direct cytotoxic activity against TU5 tumor cells in a 48 h tritiated thymidine release assay. OK-432 also stimulated the rapid (6 h, 51Cr release) macrophage-mediated killing of Actinomycin D-sensitized WEHI 164 sarcoma cells. Moreover, the expression of la antigens on peritoneal macrophages was found to be greatly enhanced after in vivo treatment with OK-432. The immunomodulatory effects of OK-432 on macrophages functions may contribute to the antitumor activity of inactivated streptococci.
1 citations