Showing papers in "International Journal of Biosciences in 2010"

Genetic Characterization of Critically Endangered Puntius sarana (Hamilton) and the Exotic Barbonymus gonionotus (Bleeker) (Cyprinidae: Cypriniformes) by DNA Fingerprinting

Abstract: Randomly amplified polymorphic DNA (RAPD) markers were used to elucidate the genetic structure of three stocks of critically endangered indigenous olive barb, Puntius sarana and one stock of exotic Thai silver barb Barbonymus gonionotus in Bangladesh. Five selected random decamer primers were used to amplify the RAPD markers of 20 fishes from each stock. The primers yielded a total of 48 RAPD bands of which 43 were considered as polymorphic (P 95 ). All the stocks showed almost similar level of intra-stock similarity indices. The population differentiation value (PhiPT = 0.698) between the indigenous and exotic barb was found to be highly significant (P<0.001). The UPGMA dendrogram showed two distinct clusters. The B. gonionotus alone made one cluster, and the remaining three P. sarana stocks made another cluster. The results showed significant genetic differences between the two species. Among the five primers, OPA 17 can be used as species-diagnostic markers to distinguish the two species. The RAPD technique has been found to be an efficient tool for genetic characterization of the two barb species to provide information on their genetic stock structure.