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Showing papers in "International Journal of Biosciences in 2016"


Journal ArticleDOI
TL;DR: The groundnut were planted at density of 44444, 66667, and 133333 hills ha-1 with average of two plants per hill in 2012 and 2013 as discussed by the authors, and the groundnut yields, harvest index, revenue, profit, and cost benefit ratio increased with increasing plant density.
Abstract: Despite the recent release of several improved varieties of groundnut in Nigeria the productivities have not increase significantly due to lack of commensurate recommendation in agronomic practices. Two groundnut varieties were evaluated for their response to different plant density and phosphorus application in two locations in the Sudan Savanna zone of Nigeria in 2012 and 2013. The groundnut were planted at density of 44444, 66667, and 133333 hills ha-1 with average of two plants per hill. Phosphorus was applied at rate of 0 or 20 kg P ha-1. P fertilizer application increased pod and haulm yields by 26% and 16% respectively in Minjibir. It increased pod and haulm yields by 62% and 27% respectively in Wudil. Pod and haulm yields, harvest index, revenue, profit and cost benefit ratio increased with increasing plant density. Samnut-24 produced pod yields that were significantly higher than Samnut-22 across treatments. Pod yields at density of 133,333 hills ha-1 was 31% higher than at 66667 and 40% than at 44,444 hills ha-1. Application of fertilizer increased profit by 22% and 49% in Minjibir and Wudil respectively. Planting at density of 133,333 hill ha-1 increased profit by 19% and 27% over 66,667 and 444444 hill ha-1 respectively in Minjibir, while it increase profit by 9% in Wudil. Cultivation of Samnut-24 at high density with phosphorus application will make groundnut production a more profitable venture in Sudan Savanna zone of Nigeria.

5 citations



Journal Article
TL;DR: For the various plant associations, the mycorrhizal soil infectivity was significantly improved and increased more than four times, and this association had a positive effect on Schinus terebinthifolius growth, phosphorus and nitrogen sheets content, in particular when this species was associated with Lotus creticus.
Abstract: Intensive exploitation of Terga sandpit situated in west of Algeria had led to degraded ecosystem. The objective of this work consists in a strategy of site revegetating by introducing native species Schinus terebinthifolius associated or not to two nitrogen fixing species Retama monosperma and Lotus creticus. Physico-chemical soil analysis and mycorrhyzal soil infectivity determination were performed on bare soil and after 24 months of planting. Endomycorrhizal structures presence or absence was recorded for each treatment. Compared to naked soil, the obtained analysis showed soil fertility evolution after two years of plants introduction. Thus, for the various plant associations, the mycorrhizal soil infectivity was significantly improved and increased more than four times. Control area mycorhization frequency was 60 % and more than 80 % for Schinus terebinthifolius associated with legumes. Hence, this association had a positive effect on Schinus terebinthifolius growth, phosphorus and nitrogen sheets content, in particular when this species was associated with Lotus creticus. * Corresponding Author: Ighilhariz Zohra  zoraighil@yahoo.fr International Journal of Biosciences | IJB | ISSN: 2220-6655 (Print) 2222-5234 (Online) http://www.innspub.net Vol. 9, No. 1, p. 148-161, 2016

1 citations


Journal Article
TL;DR: The result indicated that CVPDr gene was not givea resistant to the disease in these plants and suggested that other/s gene is needed to give aresistant or tolerant inThese plants.
Abstract: Citrus Vein Phloem Degeneration (CVPD) is the main disease of citrus plant caused by a Gram negativebacteria, Candidatus Liberobacter asiaticus. The disease is also called citrus greening disease or recentlycalled citrus huanglongbing. The CVPDr gene was firstly found in Triphacia trifoliata a citrus relativeplant which considered to be resistant to CVPD disease. The distribution of the gene among the citrusrelative plants in Bali were studied. The result of this study so far, CVPDr gene were found in theseedless lime (Citrus aurantiifolia) and Triphacia trifoliata which are known as a resistant or tolerant toCVPD disease, however, Citrus maxima, Citrus nobilis, Citrus reticulate, and Citrus amblycarpa are allsensitive plants but their harboring the CVPDr gene. This result indicated that CVPDr gene was not givea resistant to the disease in these plants. This results suggested that other/s gene is needed to give aresistant or tolerant in these plants.

1 citations


Journal Article
TL;DR: In this article, the number of PBMC (peripheral blood mononuclear cells) derived from blood samples which come in at different time was performed at the same time in order to reduce variations in results.
Abstract: Cryopreservation of PBMC (peripheral blood mononuclear cells) was done to preserve and analyze the number of PBMC derived from blood samples which come in at different time. The batch analysis wasperformed at the same time in order to reduce variations in results. The analysis on the cells numbers carried out after 1, 3, 6, 12, and 24 hours. Heparinized whole blood was collected from healthy subject by venipuncture, and stored at room temperature. Blood is processed by centrifugation in Ficoll density gradient following the established method of Balai Besar Veteriner Denpasar. Buffy coat layer was collected and washed twice with HBSS (Hank's balanced salt solution) and was counted in Turk’s solution. The cells were then dissolved in 1 ml of cold freezing medium containing 10% DMSO and 50% FBS (fetal bovine serum) and stored overnight at -80°C before storage in liquid nitrogen vessel for few weeks. The samples rapidly thawed in a water bath at 37°C and washed twice with PBS (phosphate buffered saline). The cells were stored in 4°C PBS and counted in Turk’s solution after 1, 3, 6, 12, and 24 hours. The results obtained were varied with a declining trend.

Journal Article
TL;DR: In this article, a mixture of natural pigments (Gambier- Piper betle leaves- Areca seeds) and NaCl salt was used as the mordant on the surface of Albisia wood fiber and test the physical and chemical dye properties with 1% detergent.
Abstract: Wood is one of the few materials that is highly exploited by humans, resulting in increasing usage of low quality wood. High demand and usage on high-quality wood causing heavy reduction of its availability. To increase artistic value of low quality wood, staining on the surface of the wood need to be done. This research aims to apply a mix of natural pigments (Gambier- Piper betle leaves- Areca seeds) and NaCl salt as the mordant on the surface of Albesia wood fiber and test the physical and chemical dye properties with 1% detergent. This study uses a mixture of natural dyes (Gambier- Piper betle leaves- Areca nut) with various concentrations and salt as auxiliaries, carried out with the simultaneous method of mordanting. This study was divided into 7 groups. Differences in mean between groups were tested using One Way ANOVA followed by Post Hoc LSD, which revealed statistically significant test when p value 0.05. The results showed that there were significant differences of natural dyes mixed applications (Gambier- Piper betle leaves- Areca seeds) in the ratio of 5.0: 3.0: 3.0 to 0.5 g of salt as auxiliaries and 10 minutes of immersion with the simultaneous method of mordanting. Ratio of the dye mixture, on the surface of the wood causes brown color and has the highest absorption average of 0.19 gram and the durability of the dye showed the highest seen from the appearance of color and mix of the missing mass. It can be concluded that the best application is with a mixture of natural dyes and the addition of 0.5 g of NaCl salt on the surface of Albisia wood fiber with the simultaneous method of mordanting and also based on the durability of the dye test is a ratio of 5.0: 3.0: 3.0.

Journal Article
TL;DR: The results of this study indicate that there are some isolates phosphate solubilizing rhizobacteria that are capable of dissolving the insoluble inorganic-P with different abilities, and the highest potential successively displayed by isolates TbPP-4.1 and BdPP-2.1 are significantly higher than the other isolates for producing phosphatase.
Abstract: Phosphatase in the soil was found as extracellular enzymes produced by soil micro-organisms both in acidic or alkaline conditions. Phosphatase is an enzyme complex that plays an important role in deciding soil-bound phosphate bond organic compounds to form orthophosphate which is available to the plant. Mineralization of organic forms into a inorganic-P determined by the ability of these bacteria to produce phosphatase. Therefore, observations of phosphatase activities are important to know how intensely P mineralization process takes place in the soil. Phosphate solubilizing rhizobacteria (PSR) has been widely known to affect mobilization of insoluble inorganic phosphates become available to plants. Although phosphate solubilizing rhizobacteria is widely available in the soil, their activity is usually not strong enough to compete with other bacteria commonly located in the root zone (rhizosphere). That is why, screening of phosphatase enzyme activity and various phosphate solubilizing ability to obtain better strains of bacteria are still needed. This study was conducted to determine the ability of phosphate solubilizing rhizobacteria, its potential as a biofertilizer inoculant and characterize its capacity to dissolve P-insoluble inorganic and produce phosphatase enzymes in the growing medium. Potential phosphate solubilizing rhizobacteria was tested for their ability in vitro using Pikovskaya media containing insoluble forms of inorganic phosphate as a source P. The results of this study indicate that there are some isolates phosphate solubilizing rhizobacteria that are capable of dissolving the insoluble inorganic-P with different abilities. The highest potential successively displayed by isolates TbPP-4.1; BdPP-2.1; and SBPP-1.3 that significantly has the same ability to soluble inorganic phosphate in the soil. The isolates BdPP-2.1 and TbPP-4.1 are significantly higher than the other isolates for producing phosphatase. Both isolates TbPP-4.1 and BdPP-2.1 have the same pontenty as a biofertilizer based on its capacity to mineralize forms of soil organic phosphorus by phosphatase activity.

Journal Article
TL;DR: The results showed that the treatment of 6 ppm 2-iP with up-right explant position was the most appropriate condition in stimulating growth of grapevine shoot.
Abstract: The research concerning induction of grapevine shoots grown in vitro have been conducted during period of May to July 2016 at The Laboratory of Plant Tissue Culture, Faculty of Agriculture, Udayana University. The objective of the research was to investigate the most appropriate explant position and the optimal concentration of 2-iP in stimulating of grapevine shoot in vitro. Nodal segment from grapevine plant grown in the green house was used as planting material. The experiment was laid out in the factorial design, with two factors. The first factor was the position of explant (lay-down and up-right position) and the second factor was concentration of 2-iP ( i.e. 0, 3, 6, 9, 12 ppm). Each combination was replicated ten times. The results showed that the treatment of 6 ppm 2-iP with up-right explant position was the most appropriate condition in stimulating growth of grapevine shoot. With up-right position of explant, the percentage of shoot producing-explants at 10 weeks after planting was 100% at the treatment of 6 ppm 2-iP, compared to 4%, 12%, 40%,and 40% at the treatment of 0 , 3, 9 and 12 ppm 2-iP respectively. The average number of shoot per explant was 5.5 for 6 ppm-treatment, while it was less than 3 for other treatments.