International journal of wild silkmoth & silk 

About: International journal of wild silkmoth & silk is an academic journal. The journal publishes majorly in the area(s): Saturniidae & Antheraea pernyi. It has an ISSN identifier of 1340-4725. Over the lifetime, 103 publications have been published receiving 401 citations.

Efficiency of Antheraea pernyi Nucleopolyhedrovirus-Mediated Protein Production in both an Established Cell line and Diapausing Pupae of A. pernyi

Abstract: Efficiency of recombinant protein production in a novel baculovirus expression vector (BEV) system established by combining an established cell line (AnPe) and diapausing pupae of Antheraea Pernyi with a baculovirus A. P ernyi NPV (AnpeNPV) was compared with other BEV systems and evaluated. ln vitro production using AnpeNPV/AnPe cell systems showed similar maximum B-galactosidase activity to the AcNPV/Sf9 and HycuNPV/Splm cell systems,5times more than the BmNPV/BmN4 cell system and one- fifth of the AcNPV/High5 cell system. ln vivo production using AnpeNPV/A. Pernyi diapausing pupa system showed the maximum B-galacto sidase activity similar (male pupa) to or significantly higher(female pupa)than BmNPV/Bomめrx mori larva system. The

Nucleotide Sequence Analysis of the Polyhedrin Gene of Antheraea pernyi Nucleopolyhedrovirus and Construction of a Transfer Vector Plasmid

Abstract: The polyhedrin gene of Antheraea Pernyi nucleopolyhedrovirus (AnpeNPV) was identified in a 6. 6 kbp Pstl fragment of the viral DNA genome by Southern blot hybridization using the polyhedrin gene of Bombyx mori NPV (BmNPV) as a probe. The nucleotide sequence analysis revealed that the AnpeNPV polyhedrin gene contained a promoter sequence of 51 bp and a coding sequence of 738 bp encoding 245 amino acids. Amino acid sequence comparison was made with both polyhedrins and granulins of other baculoviruses and very high homologies were detected with members of the group 1 NPV. Among them a NPV of another saturuniid moth, Attacus ricini (一 Samia cynthia ricinz) showed the highest homology, indicating that the two NPVs are closely related or variants. For the establishment of a novel baculovirus expression vector system using AnpeNPV and diapausing pupae of A. Pernyi, we have constructed a transfer vector plasmid pApCHI, which enable the foreign gene expression under the control of the strong AnpeNPV polyhedrin promoter.