journal of applied pharmaceutical science 

About: journal of applied pharmaceutical science is an academic journal published by Open Science Publishers LLP. The journal publishes majorly in the area(s): DPPH & Traditional medicine. It has an ISSN identifier of 2231-3354. It is also open access. Over the lifetime, 2425 publications have been published receiving 16571 citations.

Collagen: Animal Sources and Biomedical Application

Abstract: Collagen is the sole most profuse protein in the animal kingdom. It has been subjected to various studies from time immemorial. Its applications are numerous and have been extracted from various sources such as land animals (mainly bovine and porcine) and birds. Although collagen sources are abundant the outbreak of varied diseases among land animals posed a threat to its utilization in our daily life. Thus a probe for an alternative source began which in turn revealed the immense untapped marine source. The present article deals with a brief description of collagen its characteristics, chemistry, common extraction procedure, application in various fields and sources. A lot of studies have been carried out on various land animals, birds and marine organisms and this review sums up the work performed to date in a concise manner.

Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of North-Eastern region in India -

Abstract: A reversed-phase high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of ascorbic acid , free phenolic acids and flavonoids (catechin, rutin , quercetin, myrecetin, apigenin and Kaempferol) in four different solvent extracts of two wild edible leaves of viz. Sonchus arvensis and Oenanthe linearis, collected from North-eastern region in India . The chromatographic separation was carried out on Acclaim C 18 column (5 μm particle size, 250 x 4.6 mm) , Dionex Ultimate 3000 liquid chromatograph and detection was carried out at three different wave lengths (272 , 280 and 310 nm) using a mobile phase of acetonitrile and 1% aqueous acetic acid solution with gradient elution . The experimental results showed high amount of ascorbic acid in S. arvensis and O. linearis (1.2% and 2.3 % respectively) and gallic acid ( 0.02% and 0.06% respectively) in 1% aq. acetic acid extract of these two plants. The high percentage of recovery (96-103%), low coefficient of variation ( R2 > 0.99) and low limit of detection (LOD) and limit of quantitation (LOQ) confirm the suitability of the method for simultaneous quantification of ascorbic acid and all phenolic compounds in the two plants under investigation.

Estimation of Phenolic content, Flavonoid content, Antioxidant and Alpha amylase Inhibitory Activity of Marketed Polyherbal Formulation

Abstract: Article history: Received on: 01/07/2014 Revised on: 24/07/2014 Accepted on: 12/08/2014 Available online: 27/09/2014 The objective of this study was to evaluate αamylase inhibitory activity of a marketed polyherbal formulation. The α-amylase is one of the major secretory products helps in digestion of starch and glycogen. The polyherbal extract were prepared with aqueous, hydroalcohol and ethanol. In addition, total phenolic content, total flavonoid content and in vitro antioxidant activity was evaluated. Total phenolic content was found to be 3.5725 ± 0.2336 mg of GAE/ 100 g (aqueous extract), 2.9616 ± 0.2563 mg of GAE/ 100 g (hydroalcohol extract), 4.6683 ± 0.4199 mg of GAE/ 100 g (ethanol extract). Total flavonoid content was found to be 96.1556 ± 4.2664 mg of quercetin equivalent/ 100 g (aqueous extract), 85.1881 ± 4.2135 mg of quercetin equivalent/ 100 g (hydroalcohol extract), 96.0122 ± 2.9972 mg of quercetin equivalent/ 100 g (ethanol extract). In vitro antioxidant activity was found to be 6.436 ± 0.3638 mg of ascorbic acid equivalent / 100 g (aqueous extract), 6.7242 ± 0.2461 mg of ascorbic acid equivalent / 100 g (hydroalcohol extract), 5.4616 ± 0.6696 mg of ascorbic acid equivalent / 100 g (ethanol extract). α-amylase inhibitory activity was found to maximum in water extract followed by ethanol extract and hydroalcohol extract.

Musa paradisiaca L. and Musa sapientum L. : A phytochemical and pharmacological review

Abstract: Musa paradisiaca L. and Musa sapientum L. (Musaceae) are mainly grown in the tropical and subtropical countries and are widely used for its nutritional values all over the world. The fruits as well as the other parts of the plant are used to treat different diseases in human in traditional medicine. This review presents the scientific information on the traditional uses, phytochemistry and pharmacology of these two species. Both M. paradisiaca and M. sapientum are traditionally used in diarrhoea, dysentery, intestinal lesions in ulcerative colitis, diabetes, sprue, uremia, nephritis, gout, hypertension and cardiac disease. This review reports the phytochemicals isolated and identified from fruit pulp, peel, seeds and flowers. A comprehensive assessment of the biological activities of different extracts is included and possible mechanisms and phytochemicals involved have been correlated.

In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of Marine Yeast

Abstract: Marine yeasts isolated from coastal mangrove ecosystem namely Candida albicans, Kuraishia capsulate and Sacchromyces cerevisae were screened for the cytotoxicity against a non-transformed Vero (African green monkey kidney normal cell line) and two cancer cell lines human breast carcinoma cells (HepG2), human breast carcinoma cells (MCF -7) in different concentrations (1000 to 1.953 µg/ml).lower doses enhanced the viability of the cultured cells,MTT assay .higher doses decreased viability of the cells by 50% or more. MTT assay was used to measure the cell proliferation and survival. Of three yeast strains, Sacchromyces cerevisae showed more than 80% cell viability in Vero cell lines and were studied for further cytotoxicity against HepG2, MCF -7 cell lines respectively.