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Showing papers in "Journal of Biochemical and Biophysical Methods in 2000"


Journal ArticleDOI
TL;DR: Beta-2 microglobulin and 18S rRNA are suitable internal control genes in quantitative serum-stimulation studies, while beta-actin and GAPDH are not.

1,252 citations


Journal ArticleDOI
TL;DR: Percoll, once introduced to reduce convection during centrifugation, has proved to be the density gradientmedium of choice since it fulfills almost all criteria of an ideal density gradient medium.

211 citations


Journal ArticleDOI
TL;DR: By the use of an ordinary inkjet printer model it is shown that it is possible to print DNA onto hybridization membranes and hybridize using either specific genes or total cDNA as probes and the high resolution (300 dpi) might be used in the future to construct complex micro-arrays to analyze simultaneously large numbers of genes.

167 citations


Journal ArticleDOI
Pedro Lozano1, D Delgado1, D Gómez1, M Rubio1, J.L. Iborra1 
TL;DR: A supercritical carbon dioxide extraction method to obtain selectively volatile compounds of saffron without sample destruction has been developed and a decrease in supercritical fluid density was shown to be a critical parameter for enhancing the extraction power of carbon dioxide.

131 citations


Journal ArticleDOI
TL;DR: In this paper, a fluorescent probe for cathepsin D was synthesized, where the pepstatin A was covalently conjugated with the BODIPY (Boron dipyrromethene difluoride) fluorophore.

92 citations


Journal ArticleDOI
TL;DR: It is concluded that, at low Mg++ concentrations, different PCR systems are inhibited to varying extents by intercalated dyes and, in some PCR systems, intercalating dyes at unusually high concentrations maintain input DNA electrophoretic profile.

83 citations


Journal ArticleDOI
TL;DR: The extraction method used has low environmental impact and produced solvent-free extracts in good yield with no pigments, waxes, resins, or high-molecular weight compounds which may interfere with the isolation and analysis of the alkaloids responsible for rue's biological activity, which were extracted in relatively high yield.

71 citations


Journal ArticleDOI
TL;DR: A method of synthesizing monodisperse, magnetite-doped polymeric microspheres has been developed and it is shown that this method may predict the cell trajectory behavior in the well-characterized field environments of the authors' continuous separators.

69 citations


Journal ArticleDOI
TL;DR: This review article intends to introduce the possibility of utilizing selective supercritical fluid extraction (SFE) as a tool to study sorption/desorption processes and bioavailability of persistent organic pollutants (POP) in sediment.

66 citations


Journal ArticleDOI
TL;DR: A new method employing an atomic force microscope for nanometer-level-structure analysis of DNA damage induced with gamma-irradiation and the torsional feature of the plasmid DNA was visualized better with AFM than with a transmission electron microscope (TEM).

55 citations


Journal ArticleDOI
TL;DR: Antibodies immobilized via a PEG spacer showed a higher capture efficiency compared to direct immobilization, which was more pronounced with large antigens, including Bacillus globigii, E. coli O157:H7, and ovalbumin.

Journal ArticleDOI
TL;DR: An analytical SFC was used to screen the performance of a wide range of mobile and stationary phases for the elution of more than 60 miscellaneous small drug compounds and was shown to be a good predictor of the semi-prep instrumental performance.

Journal ArticleDOI
TL;DR: Analytical and preparative supercritical fluid chromatography (SFC) were used for investigation of myrosinase catalysed degradation of 4-hydroxybenzylglucosinolate (sinalbin) and determination comprised transformation products of sinalbin, produced both during degradation of isolated sinal bin, and during autolysis of meal from S. alba seeds.

Journal ArticleDOI
Zhao Ruming1, Liu Yi1, Xie Zhixiong1, Shen Ping1, Qu Song-Sheng1 
TL;DR: A microcalorimetric technique based on the bacterial heat-output was explored to evaluate the stimulatory effect of La(3+) on Escherichia coli, and agreed with the conventional methods, such as cell numbers and biomass.

Journal ArticleDOI
TL;DR: Overall it is shown that supercritical fluid technology is fast becoming a major part of forensic investigations and that it is an invaluable analysis technique.

Journal ArticleDOI
TL;DR: Emmphasis is placed on analysis of samples with a high water content containing polar pesticides, with particular attention paid to the multiresidue analyses.

Journal ArticleDOI
TL;DR: In this article, the properties of the multiple forms of human hyaluronidases in somatic tissues and in body fluids were investigated, and potential N-glycosylation sites with similar tripeptide patterns were observed at the same position in human plasma.

Journal ArticleDOI
TL;DR: The reduced MI content in the sciatic nerve and lens of streptozotocin-induced diabetic rats recovered in a dose-dependent manner by treatment with a novel potent aldose reductase inhibitor, GP-1447.

Journal ArticleDOI
TL;DR: Although the application of methanol-modified supercritical CO(2) was a time-saving extraction procedure which reduced solvent usage and solvent waste, SFE efficiency proved only competitive to conventional slurry and Soxhlet extraction.

Journal ArticleDOI
TL;DR: A method has been developed for the determination of vitamins A and E in food using supercritical fluid extraction (SFE), applying liquid or solid trapping, with an accuracy equal to conventional solvent extraction methods, under optimal conditions.

Journal ArticleDOI
TL;DR: Investigation of the autolysis products of white cabbage showed that ascorbigens were the quantitatively dominating degradation products of indol-3-ylmethylglucosinolates, and a method using normal-phase supercritical fluid chromatography with methanol as modifier has been developed for determination and quantification.

Journal ArticleDOI
TL;DR: An optimisation procedure for the supercritical fluid extraction of cocaine from the leaves of Erythroxylum coca var.

Journal ArticleDOI
TL;DR: The effects of the chromatographic parameters were studied for the separation of steroids and the successful results were shown and compared with HPLC.

Journal ArticleDOI
TL;DR: The separation of ketoconazole enantiomers by subcritical-fluid chromatography using an amylose-based column is described and drastic changes in the resolution have been obtained for the different organic modifiers evaluated, with ethanol providing the best results.

Journal ArticleDOI
TL;DR: Chiral, liquid crystal and polymer-based stationary phases coated on particles are widely covered in this review as an interesting approach to separate polar compounds avoiding the major drawbacks associated to the use of modifiers in SFC.

Journal ArticleDOI
TL;DR: Packed column SFC is most useful for the separation of mixtures usually separated by normal-phase HPLC and offers faster separations, higher efficiencies, faster column re-equilibration, and a wider range of experimental variables for optimization.

Journal ArticleDOI
TL;DR: Data is presented on automated, size-based separation of DNA fragments by ion-pair reversed-phase high performance liquid chromatography (IP RP HPLC) - DNA chromatography - on the WAVE DNA Fragment Analysis System with the DNASep cartridge.

Journal ArticleDOI
TL;DR: The microcalorimetric bioassay for acute cellular toxicity is based on metabolic heat production from cultured cells and the effect of Cd(2+) on Rhizopus nigricans growth was investigated at 25 degrees C.

Journal ArticleDOI
TL;DR: Various vitamins were well separated using water-modified supercritical CO(2) fluid using an amperometric microsensor made of a thin film of perfluorosulfonate ionomer.

Journal ArticleDOI
TL;DR: This paper describes the quantitative evaluation of a bioluminescence assay for DNA damaging agents with respect to the linearity, sensitivity, specificity and dependence on the cell culture status of a recombinant bacterium, DPD2794, which showed a very sensitive response to DNA-damaging stress.