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Showing papers in "Journal of Biological Chemistry in 1940"





Journal ArticleDOI
TL;DR: The purpose was to evaluate the method by investigating the optimum conditions for the color reaction, its specificity, and its correlation with the capon assay for androgens by evaluating the details of various modifications of Zimmermann's calorimetric method.

287 citations


Journal ArticleDOI
TL;DR: A quantitative method of analysis of organic compounds in mixtures based on the fact that a compound which has an abnormal isotope content is inseparable by the usual laboratory procedures from its normal analogue is described.

284 citations




Journal ArticleDOI
TL;DR: The primary purpose of this paper is to establish satisfactory criteria for several chlorophyll preparations, especially with reference to their absorption of light, of fundamental importance in assays of pigment mixtures by spectroscopic means.

172 citations


Journal ArticleDOI
TL;DR: Both of these substances may be considered impurities and should be eliminated, if possible, in the purification of theprotein, especially since the presence of the nucleic acid modified many of the physicochemical properties of the protein, such as isoelectric point, electrophoretic mobilities, and solubilities.

151 citations


Journal ArticleDOI
TL;DR: The apparent clearance of endogenous chromogenic substance, either with or without correction, has been recommended as a measure of glomerular filtration, but, such endogenous clearances cannot in the authors' opinion be accepted for this purpose.

149 citations





Journal ArticleDOI
Philip P. Cohen1
TL;DR: The author found that transamination in pigeon breast muscle is limited to the following reactions.





Journal ArticleDOI
TL;DR: In this investigation lipid metabolism was studied during the time of its greatest activity in nervous tissue-the period known to morphologists as that of myelination.

Journal ArticleDOI
TL;DR: The amount of fluorine picked up by these calcium phosphates was found to follow the adsorption isotherm, thus demonstrating that fluorine does adsorb on dental tissues.

Journal ArticleDOI
TL;DR: The excretion of homogentisic acid by rats after administration of large doses of phenylalanine and by guinea pigs and humans after ingestion of tyrosine seems to indicate that both amino acids travel similar pathways during degradation.

Journal ArticleDOI
TL;DR: A further simplification of the procedure for globulin precipitation was made based on the use of ether to decrease the density of globulin precipitated by sodium sulfate, which showed that globulin could be salted-out at 25’ as well as at 38”.

Journal ArticleDOI
TL;DR: The study of the precursors of creatine in animals has been beset by two difficulties principally by the lack of really adequate biological material and the absence of a specific, and at the same time sensitive analytical method.




Journal ArticleDOI
TL;DR: Of greater significance was the finding that’ these metabolites as in the case of the homogentisic acid are no longer excreted when an adequate amount of ascorbic acid is administered to the guinea pigs.


Journal ArticleDOI
TL;DR: The excretion of ascorbic acid by albino rats maintained as previously described was greatly accelerated by a series of compounds, most of them having in common the characteristic of functioning in vivo as depressors of the nervous system.

Journal ArticleDOI
TL;DR: The present paper deals with the effect of various agents on sulfhydryl groups, as determined by the porphyrindin titration, and the effects of these agents on viscosity, double refraction of flow, and solubility.