Showing papers in "Journal of Biological Chemistry in 1941"

TL;DR: This paper deals with the estimation of chlorophyll in plant extracts by application of absorption coefficients of the isolated solid chlorophylla components, and the question of artifacts is automatically clarified.

TL;DR: It has been concluded that the mercurimetric determination of chloride offers an especially favorable method for a rapid, simple, and accurate determination.

TL;DR: In this article, the enzyme activity of all enzyme preparations was determined by measurement of leucyldiglycine hydrolysis, and the peptidase unit employed, which was identical with the unit used in previous papers, may be defined as the amount of enzyme which will bring about 50 per cent hydrolyisation of I-peptide in 30 minutes.

TL;DR: The method here presented depends on the fact that a-amino acids, when boiled in water with an excess of ninhydrin (triketohydrindene hydrate) at pH 1 to 5, evolve the CO2 of their carboxyl groups quantitatively in a few minutes.

TL;DR: In continuing the studies in this laboratory on new factors required by growing chicks, the authors have encountered a severe choline deficiency and chicks upon their basal ration show inferior growth and severe perosis which are entirely prevented by the addition of 0.1 per cent choline to the diet.

TL;DR: This paper presents data on the distribution of acid-soluble phosphorus compounds in the blood cells of a few apparently normal individuals of each of forty-six species: twenty-two mammalian, twelve avian, six reptilian, four amphibian, and two fish.

TL;DR: A crystalline dimethyl other (C12H12O4(OCH2)2 with a melting point of 168-170° (physiologically inactive) has been prepared by methylation with diazomethane as mentioned in this paper.

TL;DR: A number of years ago, while making a study of the behavior of various compounds with the oxidized nitroprusside reagent of Weber (l), Sullivan found that methionine, 0.2 mg. per cc., gave with this reagent a strong yellow color which after 5 to 10 minutes standing, became a strong red on acidification as discussed by the authors.

TL;DR: Evidence to be presented in this paper indicates that in human blood the potassium and sodium distribution between the~11s and the extracellular medium is greatly influenced by the mctabolism of the erythrocytes, and that the factors operative in the original accumulation of potassium within the ~11s arc probably responsible for the maintenance of the concentration gradient normally observed.

TL;DR: The present report deals with the preparation of an insoluble fraction from fresh yeast which can inactivate, specifically, the third component of complement.

TL;DR: The transfer of methyl groups from methionine to choline and creatine has been demonstrated by the isolation of deuteriocholine and deuteriocreatine from the tissues, and of deutriocreatinine from urine, or rats fed methionines containg a deuterio-methionine as mentioned in this paper.

TL;DR: It was decided that in the meantime it would be worth while to repeat the work of Kiister and Irion with the hope of isolating the thio ether from wool, and familiarity gained with its properties should be of value later when work on the analogous selenium-sulfur compound is resumed.

TL;DR: In the present method, the CO2 evolved by decarboxylating amino acids with ninhydrin is transferred to standard barium hydroxide and titrated as mentioned in this paper.

TL;DR: The fate of bicarbonate carbon,l (+4) C, in the rat is concerned, and an average of 0.6 per cent of the radioactivity administered appeared in the liver glycogen, indicating that CO2 may be intimately concerned as an intermediary in carbohydrate metabolism of mammalian organisms.

TL;DR: The occurrence of lipids in serum prot(eins separated by the aid of electrophoresis has earlier been investigated by Bennhold and Mellander and the discrepancy between the results may most easily be explained on the assumption that the test for cholesterol was not sensitive enough to detect this substance in the separated albumin.

TL;DR: The vast literature on creatine formation is presented, which has recently been completely reviewed, and the recent revealing experiments of Borsook and Dubnoff on arginine (S), in conjunction with the own experiments are discussed.

TL;DR: It appeared probable that the substance being concentrated which rendered the biotin unavailable to yeast was identical with the constituent in raw egg white responsible for the injury to animals.

TL;DR: It was shown in preceding communications that glycocyamine is converted into creatine by surviving liver slices, but this work found no evidence of this methylating mechanism in any other tissues, except possibly slight activity in the kidney.