Showing papers in "Journal of Biological Chemistry in 1951"
•
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
289,852 citations
••
TL;DR: In this article, the separation of amino acids by elution analysis on columns of synthetic ion exchange resins has been investigated and shown to have a resolving power comparable with that obtained with columns of starch or paper.
1,500 citations
••
TL;DR: A microadaptation enables the measurement of the cytochrome oxidase content of as little as 4 y (wet weight) of an active tissue such as heart.
1,294 citations
••
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
1,203 citations
••
TL;DR: To 1.0 cc.
1,016 citations
••
707 citations
••
TL;DR: The objective of the present work was to provide a means for separating and indentifying phosphate esters involved in glycolysis in higher plants using two-dimensional chromatography with successive development in an acid and in a basic solvent.
675 citations
••
576 citations
••
538 citations
••
TL;DR: Attempts to identify this “activator” function of microbial extracts led to the proposition that the phosphate exchange and arsenolysis system may be responsible for the acetyl transfer from acetyl phosphate.
473 citations
••
TL;DR: The determinations reported here followed a conventional alcohol-ether extraction and subsequent reextraction in petroleum ether, and it has been found that urea, even in amounts considerably in excess of what might occur, produces no color change in the dichromate reagent.
••
TL;DR: In order to provide some definitive information on the mechanism of conversion ofadenosine to adenosine-5’-phosphate, an enzyme preparation has been obtained which catalyzes the reaction.
••
TL;DR: It is shown that the ability to form a complex with cyanide is a general property of N-substituted nicotinamide compounds.
••
••
••
••
••
••
TL;DR: A procedure for the fractionation of complex corticoid mixtures and a systematic method for the identification of the separated compounds based upon these techniques are described and offer several advantages over those used by previous investigators.
••
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
••
••
••
TL;DR: The procedure here described has been adopted after numerous experiments in which many combinations of solvents were tried, and it is hoped that this product can be universally substituted for beef heart lecithin in cardiolipin antigens.
••
TL;DR: The biological conversion of d-isocitric to a-ketoglutaric acid was established by the work of Martius and others and Adler et al. showed that, with extracts of acetone powders of animal tissues, triphosphopyridine nucleotide (TPN) is a specific coenzyme in the reaction and cited evidence for a similar specificity in yeast.
••
••
TL;DR: It has been found advantageous to use as a fluid a mixture of H&S04 and HsPOa without CrOs, both KI03 and KzCrzOr being added in solid form in the combustion tube, for carbohydrates and polyhydroxyl alcohols.
••
TL;DR: It will be shown in this paper that the first step, the phosphorylase reaction, is rate-limiting and that the H-G factor and epinephrine exert their effect by causing a rapid increase in the phosphORYlase activity of the liver.
••
••