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Showing papers in "Journal of Biological Chemistry in 1957"


Journal ArticleDOI
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.

59,550 citations



Journal ArticleDOI
TL;DR: One of the interesting features associated with the administration of 2deoxyglucose to eviscerated animals is that it leads to a marked reduction in the intracellular transfer of glucose despite the injection of maximal amounts of insulin, suggesting that 2-deoxyGLucose may be considered a metabolic block for glucose.

745 citations


Journal ArticleDOI
TL;DR: The most plausible route for the conversion of hemoglobin into biliverdin in vivo is through the oxidation and opening of the tetrapyrrole ring, after which the globin and iron are removed as discussed by the authors.

722 citations


Journal ArticleDOI
TL;DR: It has been found practicable with a fluorometer to determine separately oxidized and reduced pyridine nucleotides and the useful range of sensitivity is about 2000-fold, which is much greater than that obtained with calorimetry.

516 citations


Journal ArticleDOI
TL;DR: Data is presented here on the induced synthesis of a benzpyrene-metabolizing system by 3,4-benzpyrene and related hydrocarbons, which occurs in the liver microsomes and requires TPNH and oxygen for activity.

464 citations



Journal ArticleDOI
TL;DR: The present investigation was undertaken in search of a reducing agent possessing the high sensitivity and rapidity of color development characteristic of stannous chloride and the high order of stability characteristic of ANS.

436 citations


Journal ArticleDOI
TL;DR: Studies now indicate that FA and FAH:! are reduced to FAHJ with reduced pyridine nucleotides in the presence of an enzyme system partially purified from chicken liver.

398 citations


Journal ArticleDOI
TL;DR: The present communication deals with the isolation, purification, and characterization of the ribon nucleic acids which are soluble in sodium chloride, and also describes the isolation and certain properties of a fifth nucleotide component which became evident in studying the action of ribonuclease on these ribonucleic acids.

354 citations


Journal ArticleDOI
TL;DR: The present investigation concerns the identification and quantitative determination of the methyluric acids and methylxanthines excreted in the urine of man after the ingestion of theobromine, theophylline, and caffeine.




Journal ArticleDOI
TL;DR: In this paper, emission spectrogra,phy has been adapted to the simultaneous determination of several metals in fractions from livers of normal rats, and it has been found that each metal is reproducibly distributed among the fractions, and that each fraction shows a characteristic and significantly different pattern of metal concentration.

Journal ArticleDOI
TL;DR: Investigation of the mechanism of action of ACTH revealed that the adrenal stimulation is accompanied by an increase of phosphorylase activity in the tissue, which is in accord with the well established fact that ACTH in tivo causes the Adrenal cortex to secrete increased quantities of the adrenAL steroid hormones.


Journal ArticleDOI
TL;DR: S steroid C-21 hydroxylation has the significance of an essential step in the synthesis of steroid hormones by t’his gland as a key intermediate in adrenal cortical hormone biogenesis.


Journal ArticleDOI
TL;DR: Evidence is presented which indicates that fatty acid synthesis occurs in the soluble cytoplasmic portion of the rat liver cell and the cofactor requirements and probable enzymatic pathway of this synthetic process will be presented and discussed.


Journal ArticleDOI
TL;DR: The present paper deals with the purification of the dehydrogenase from beef heart mitochondria and surveys some of its salient catalytic properties and protein characteristics.

Journal ArticleDOI
TL;DR: Lipoprotein lipase has now been obtained from chicken adipose tissue; some of the properties of the enzyme from this source are described in this paper.

Journal ArticleDOI
TL;DR: Detailed information is provided on the site and mechanism of the inhibitory act,ion by this compound, together with more recent data on the action of 6-diazo-5-oxo-n-norleucine on inosinic acid biosynthesis.

Journal ArticleDOI
TL;DR: The chiral stationary phase for high-performance liquid chromatography showed good results in both the high-resolution and the low-resolution phases, indicating good chiral recognition ability in the liquid phase.

Journal ArticleDOI
TL;DR: Evidence will be presented that uridine diphosphate-N-acetylglucosamine’ acts as a glycosyl donor in the synthesis of chitin by a cell-free preparation from Neurospora crassa.




Journal ArticleDOI
TL;DR: Both enzymatic synthesis and degradation of deoxyribonucleic acids have as yet involved only derivatives with 5'-monoesterified phosphate.