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Showing papers in "Journal of Biological Chemistry in 1958"



Journal ArticleDOI
TL;DR: The properties of an enzyme that transfers the methyl group of S-adenosylmethionine to the hydroxyl group in position 3 of epinephrine and other catechols are described.

1,248 citations



Journal ArticleDOI
TL;DR: Information obtained from this study and from previous investigations of the properties of silicic acid were used to elaborate a standardized method for the separation of lipide groups based on adsorption chromatography with silicIC acid.

815 citations



Journal ArticleDOI
TL;DR: The intermediate stages between amino acid activation and final incorporation into protein in the rat liver in vitro system offered unexplored regions in which to seek more direct evidence for a chemical association of RNA and amino acids.

691 citations



Journal ArticleDOI
TL;DR: An investigation was undertaken to determine which structural features of tryptophan are responsible for its binding, and to elucidate the nature of the binding site of the protein.

616 citations



Journal ArticleDOI
TL;DR: Crystalline barium glucose g-phosphate was obtained from the Sigma Chemical Company, St. Louis, and intestinal phosphatase from Armour and Company, Chicago and the phosphat enzyme was dialyzed overnight against cold distilled water before use.

418 citations






Journal ArticleDOI
TL;DR: This method uses the double extraction technique of Silber and Porter and a modification of the fluorescence technique described by Sweat for analysis of plasma corticosteroids in the rat.




Journal ArticleDOI
TL;DR: It was possible to establish with reasonable certainty that the defect in alcaptonuria is limited to the enzyme, homogentisic acid oxidase, that there is a failure to synthesize active enzyme, and that the metabolic block is essentially complete.

Journal ArticleDOI
TL;DR: The enol tautomers of the aromatic oc-keto acids combine instantaneously and reversibly with borate to form complexes of a mixed ester-anhydride structure, thereby displacing the apparent keto-enol equilibrium in favor of the enl tautomer.

Journal ArticleDOI
TL;DR: Experiments indicated that 3’) 5’-AMP does indeed serve as an intermediate agent in the ACTH-induced stimulation of phosphorylase.


Journal ArticleDOI
TL;DR: The biosynthesis of spermidine has been demonstrated in cell-free preparations of E. coli and of A. nidulans, and isotopitally labeled putrescine was incorporated into these polyamines in several microorganisms.



Journal ArticleDOI
TL;DR: By using crystalline phosphorylase b it has been possible to obtain quantitative data with reference to the number of moles of phosphate introduced per mole of phosphoryLase a and to determine the nature of the nucleotide product in the phosphory lase b to a conversion.



Journal ArticleDOI
TL;DR: In the present study, the metabolism of inositol was investigated by means of a spectrophotometric method for the determination of inposol and by the use of tritium-labeledInositol.

Journal ArticleDOI
TL;DR: The Hestrin method is modified so that enzyme rates at optimal substrate concentrations of either TAME or LME can be determined by using the same standard reagents and procedures and the rate measurements are reproducible with an average deviation of 3 per cent of the rate.