Showing papers in "Journal of Biological Chemistry in 1959"

TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.

TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.

TL;DR: The separation of peptides from controlled proteolytic digests by two-dimensional paper chromatography and electrophoresis is finding wide application to studies of protein structure.

TL;DR: Neither S-peptide nor S-protein alone shows appreciable ribonuclease activity, but when these components are mixed in equimolar proportions, almost the full enzymic activity is recovered.

TL;DR: The present report describes the conversion of androgenic steroids to estrogens in high yield by a system consisting of human placental microsomes, the reduced form of triphosphopyridine nucleotide, and oxygen.

TL;DR: The hypothesis that the mechanism of action of penicillin is related to inhibition of a step in biosynthesis of the bacterial cell wall is led by the findings of a careful study of the composition of the cell walls of two strains of S. aureus.

TL;DR: The results indicate that the primary action of e-aminocaproic acid is to inhibit the activation of plasminogen, but that it also possesses, depending upon its concentration, the dual property of either inhibiting or enhancing the action of plAsmin.

TL;DR: This report describes an enzymatic assay for the determinaton of creatine and its application to creatine measurement in serum and urine.

TL;DR: In muscle extract the effect of adenosine 3’,5’-phosphoric acid is in the activation of phosphorylase b kinase, which is shown to be related to an important control process.

TL;DR: The present study was undertaken to provide a systematic scheme of analysis for the quantitative determination of the levels of these enzymes in the livers of ureotelic animals.

TL;DR: The fact, that the pH optimum for the reaction leading to the formation of imidazole carboxymethyl ribonuclease is around pH 5.5 to 6, instead of at the more alkaline pH values observed for the alkylation of cy-N-acetylhistidine, suggests that inactivation takes place as a result of substitution on a particular one of the four histidine residues present in rib onuclease.

TL;DR: The first reaction product of tyramine, horse-radish peroxidase, and hydrogen peroxide has been shown to be 2,2”-dihydroxy-5,5’-bis(ðylamino) diphenyl (I), referred to as dityramine.

TL;DR: Conjugates of bovine serum albumin with progesterone, deoxycorticosterone, and estrone have now been synthesized as an extension of a program to prepare and characterize steroid-protein conjugates with the ultimate purpose of testing their ability to stimulate the formation of antihormonal antibodies.

TL;DR: The data presented in this paper show that the enzyme, when dissociated, is inactive, and that the coenzymes DPN, DPNH, TPN and TPNH influence the sedimentation behavior of the enzyme at levels which are identical with the coenzyme concentrations used in the determination of the kinetic constants.

TL;DR: The data reported here show that rat adrenal sections which respond to ACTH will also respond to 3’, 5’-AMP with an increase in corticoid output, in harmony with the concept that 3�', 5'-AMP serves as an intermediate agent in the ACTH-induced stimulation of phosphorylase.

TL;DR: It is found that concentrated urea both stimulates and inhibits the action of ribonuclease upon ribonucleic acid (depending upon the assay procedure); some of the findings are summarized in this paper.