Showing papers in "Journal of Biological Chemistry in 1970"

TL;DR: It is demonstrated that successful application of affinity chromatography in many cases will critically depend on placing the ligand at a considerable distance from the matrix backbone.

TL;DR: It was shown that at any given pH and oxygen tension, the amount of univalently reduced oxygen, which was detectable in terms of the reduction of cytochrome c, rose as the turnover rate of the enzyme was decreased by decreasing the concentration of xanthine.

TL;DR: The data suggest a possible model for the conformation of membrane proteins and their interactions with phospholipid, and the empirical observation that proteins dissolved in aqueous solutions containing high concentrations of sodium dodecyl sulfate have electrophoretic mobilities on polyacrylamide gels which are a unique function of their molecular weights.

TL;DR: The existence of a microsomal ethanol-oxidizing system, especially its capacity to increase in activity adaptively after ethanol feeding, may explain various effects of ethanol, including proliferation of hepatic smooth endoplasmic reticulum, induction of other hepaticmicrosomal drug-detoxifying enzymes, and the metabolic tolerance to ethanol which develops in alcoholics.

TL;DR: An adenosine triphosphatase has been purified approximately 6-fold from sarcoplasmic reticulum through the use of deoxycholate and salt fractionation and the rate of ATP-ADP exchange and the level of phosphorylation are both increased by a factor commensurate with the increase in ATPase activity.

TL;DR: Biological testing showed that the sialogogic peptide isolated is indistinguishable from Substance P, and it stimulates contraction of the guinea pig ileum and the rat duodenum and is a potent vasodepressor.

TL;DR: The results have led to the conclusion that xanthine oxidase generates O2- and H2O2 as the primary products of the reduction of oxygen and generates ·OH as a secondary consequence of the interaction of O1- with H1O2 and that it is the hydroxyl radical rather than the superoxide anion radical which reacts with methional to generate ethylene.

TL;DR: The apparent location of the glycosyltransferases in the Golgi apparatus suggests that these enzymes may be involved in terminating the synthesis of plasma glycoproteins by the liver during secretion, and may possibly be required for secretion of these proteins.

TL;DR: It is suggested that the question of hysteresis in enzyme systems as defined here be systematically investigated in regulatory enzymes and that this concept may be of value in discussing the regulation of complex processes in vivo.

TL;DR: The cold-insoluble globulin of human plasma has been highly purified and there was no evidence of any alteration of the electrophoretic, ultracentrifugal, or NH2-terminal characteristics of CI-globulin following thrombin treatment or heating to 56° for 5 min.

TL;DR: The bacterial superoxide dismutase, which catalyzes the disproportionation of univalently reduced oxygen, has now been purified from Escherichia coli and was found to contain manganese.

TL;DR: These mitochondria exhibited good respiratory control and respiratory rates with various substrates, comparable with other mammalian mitochondria, and increasing the K+ in the incubation medium caused an increase in the oxygen uptake with all the above substrates (except α-glycerophosphate).

TL;DR: In order to obtain complete modification of the sulfhydryl groups in seed meals, a longer reaction time, 4.5 hours, and a concentration of 4-vinylpyridine equivalent to 3 times the mercaptoethanol concentration were required.

TL;DR: The procedures used in the collection and analysis of x-ray diffraction data from single crystals of ribonuclease-S are described and a preliminary list of the coordinates of all nonhydrogen atoms in this enzyme has been prepared.

TL;DR: Two distinct substrate binding sites on the enzyme are indicated, one with a high affinity for aromatic compounds including phenolic substrates, the other for metal-binding agents and oxygen, which suggest a sequential mechanism for the binding of catechol and oxygen.

TL;DR: Results show that the lipid is essential for the enzymatic reduction of hemoprotein P-450 and that only the rapid phase of the reaction is adequate to support the observed rate of substrate hydroxylation.

TL;DR: Studies of viscosity and circular dichroism and dialysis experiments indicate that the active glycoproteins are expanded molecules, a property which undoubtedly is of importance to their function.

TL;DR: Double stranded DNA serves as a template primer for Escherichia coli DNA polymerase when the DNA contains a single strand break (a nick) with a 3'-hydroxyl terminus and the ability of the enzyme to promote hydrolysis and synthesis simultaneously results in the translation of the nick along the DNA duplex in the 5' to 3' direction.

TL;DR: It is suggested that gel chromatography can be used as a substitute for other hydrodynamic measurements in the determination of gross conformation of proteins in these solvents.

TL;DR: The results demonstrate the absence of intracellular membranes and suggest that the outer membrane of the platelet is similar to plasma membranes of other cells, and they provide biochemical confirmation of its origin from the plasma membrane ofThe megakaryocyte.

TL;DR: In this paper, eight different substances were isolated in crystalline forms by ion exchange chromatography of the aliphatic basic amino acid fraction of human urine and the identities of the compounds were confirmed by synthesis followed by comparison of infrared and nuclear magnetic resonance spectra and of chromatographic properties.

TL;DR: Evidence is presented that the cyclic AMP-dependent protein kinase acts directly on glycogen synthetase I and not through a second kinase as occurs in the phosphorylase activation system, and that the enzyme probably exists as a tetramer composed of subunits having a molecular weight between 90,000 and 100,000.

TL;DR: A protein which inhibits mitochondrial ATPase has been isolated from bovine heart mitochondria in pure form and its properties have been characterized and it was necessary to incubate the protein with ATPase in the presence of Mg++ and ATP.

TL;DR: It is concluded that the best preparations of xanthine oxidase so far obtained contain only 75 to 80% functional active sites, even though they possess their full complement of flavin, molybdenum, and iron-sulfur components.

TL;DR: All three preparations were essentially identical in terms of their physical, chemical, and enzymatic characteristics strongly suggesting that the protein-glycogen complex represents a structural and functional unit of the cell rather than artifacts resulting from a given isolation procedure.

TL;DR: The results show similarity between the patterns of solubilizing effects of guanidine hydrochloride and urea, although Guanidine Hydrochloride is 2 to 3 times more effective than urea at the same concentrations.

TL;DR: The effectiveness of the alcohols as protein denaturants increases with increasing chain length or hydrocarbon content, in conformance of what is expected of the disorganization of the hydrophobic interior of these proteins revealed by their detailed three-dimensional x-ray structure.

TL;DR: An enzyme which catalyzes the phosphorylation of histone by ATP, and is activated by low concentrations of guanosine 3',5'-monophosphate, has been found in lobster muscle and partially purified.

TL;DR: A highly purified glycopeptide with potent phytohemagglutinin (PHA) receptor site activity has been isolated from human erythrocyte membranes, showing that the inner core sugars can influence PHA-inhibitory activity.

TL;DR: The spontaneous increase in valine accumulation was ascribed to an enhancement of proteolysis and the inhibitory effect of insulin in vitro completely inhibited this spontaneous net increase and also inhibited the release of label from previously labeled livers.