Showing papers in "Journal of Biological Chemistry in 1972"

TL;DR: It is proposed that the autoxidation of epinephrine proceeds by at least two distinct pathways, only one of which is a free radical chain reaction involving O2- and hence inhibitable by superoxide dismutase.

TL;DR: Sensitive and reproducible radioimmunoassays have been developed for cyclic adenosine 3',5'-monophosphate (cAMP), cyclic guanosine 3,5'- monophosphates (cGMP), cycled inosine 3,'5'-MonophosphATE (cIMP), and cyclic uridine 3',4'-monophile (cUMP) based upon competition of the cyclic nucleotide with isotopically labeled cyclic

TL;DR: Preliminary analysis of the phospholipid composition of the isolated fractions of Salmonella typhimurium showed significant quantitative differences in the relative distribution of the major glycerophosphatides.

TL;DR: Epidermal growth factor is a signaling molecule that stimulates the growth of epidermal tissues during development and throughout life and led to the 1986 Nobel Prize in Physiology or Medicine awarded to Cohen and Rita Levi–Montalcini.

TL;DR: A new procedure has been devised for the preparation of pure epidermal growth factor in high yield through a two-step column fractionation of crude extracts of the submaxillary gland obtained from adult male mice.

TL;DR: It is proposed that a cyclic, energy-dissipating flux of mitochondrial calcium accounts for uncoupling by A23187, whereas inhibition of ATPase and glutamate oxidation and increased potassium permeability of mitochondria result from antibiotic-mediated magnesium efflux.

TL;DR: It was concluded that adenosine triphosphate was activating the enzyme in a fashion functionally distinct from its action as a phosphate donor, since the concentration of adenosines triph phosphate required for activation was much higher than that required for phosphorylation.

TL;DR: In this modification of the 5-dimethylaminonaphthalene-1-sulfonyl-Edman degradation, inorganic carriers permit convenient manipulation of small amounts of protein, and use of the detergent sodium dodecyl sulfate throughout the procedure maintains protein solubility.

TL;DR: Crystalline wheat germ agglutinin was prepared from unprocessed wheat germ by a new purification procedure and its purity and some of its molecular characteristics were examined by a number of criteria.

TL;DR: Some potential applications of these analogues in studies of the structure and function of nucleic acids and proteins are: analysis of protein-nucleic acid interaction, polynucleotide conformation and conformational transitions, enzyme-coenzyme interaction, and nuclease assay.

TL;DR: A study of the variables in techniques for alkaline hydrolysis of proteins and for chromatographic analysis of the products has led to a method for the accurate determination of tryptophan which is applicable to foods and has been tested on normal and opaque-2 maize meals and on wheat flours.

TL;DR: The results suggest that the function of the 3' → 5' exonuclease activity of DNA polymerases is to remove mispaired nucleotides which have been incorrectly incorporated, thereby increasing the fidelity of template copying.

TL;DR: Hormonal stimuli causing marked increases in cAMP in vivo or in vitro did not affect the level of cGMP, indicating that the concentration of each of these nucleotides is controlled independently.

TL;DR: The complete amino acid sequence of epidermal growth factor (EGF) has been established through the use of automated Edman degradation and standard enzymatic and chemical techniques and is in agreement with the molecular weight of 6400 established by physical measurements.

TL;DR: Results have been interpreted to indicate that the membrane factor is made by the mitochondrial protein-synthesizing system and that its synthesis is stimulated by products of the cytoplasmic ribosomal protein-Synthesized system.

TL;DR: Study on the substrate specificity with ribonuclease as the substrate revealed that the staphylococcal protease specifically cleaves peptide bonds on the COOH-terminal side of either aspartic acid or glutamic acid.

TL;DR: EDTA had no effect on the enzyme-catalyzed dismutation of superoxide radicals, but did eliminate catalysis by Cu++, and Boiling the enzyme or reversibly removing its copper prosthetic group caused loss of catalytic activity.

TL;DR: The apparent heat capacities of the lipids in the liquid crystal state are, with the possible exception of dipalmitoyl lecithin, no more than 5 cal deg-1 (mole of lipid)-1 larger than in the gel state, indicating that the hydrocarbon chains have much less mobility in theLiquid crystalline statethan in the corresponding liquid normal paraffins.

TL;DR: Equilibration of the same lipoproteins with glycosaminoglycan-substituted agarose gels at pH 7.4 showed that VLDL as well as LDL were bound to gels containing heparin, dermatan sulfate, heparan sulfates, and chondroitin 4-sulfate, provided the ionic strength was sufficiently low.

TL;DR: The stoichiometry of the reaction catalyzed by tryptophan hydroxylase in the presence of tetrahydrobiopterin has been determined and it is suggested that catalase or Fe2+ protects a sensitive component of the tryptophilehydroxylation system from inactivation by H2O2.

TL;DR: Interaction of insulin with liver plasma membranes is a complex phenomenon that involves at least two processes, degradation and binding to receptors, and these two processes are largely independent and unrelated under a variety of conditions.

TL;DR: Methods are presented for preparation of extracts from cultured cells from the nervous system and for study of choline acetyltransferase, acetylcholinesterase, glutamate decarboxylase, and catechol O-methyltransferase activities, which are markers that can be used for studying gene expression in neurons.

TL;DR: FMN and flavodoxin are shown to cause both univalent and divalent reductions of oxygen, and the reduction of oxygen by native milk xanthine oxidase may well be a function of its non-heme iron centers.

TL;DR: The cytoplasmic progesterone-binding proteins of chick oviduct are shown to consist of two major 4 S fractions which occur in equal amounts, suggesting that the same class of hormonebinding site is involved on both A and B.

TL;DR: Lipophilic protein moieties of human low density lipoprotein, Semliki Forest virus envelope, and human erythrocyte stroma were obtained lipid-free with the use of sodium deoxycholate treatment and gel filtration in de oxygencholate-containing media to determine detergent binding.

TL;DR: The results indicate that the cytochrome P-450 and P-448 fractions have different substrate specificities and are consistent with the view that cytochromes P- 450 andP-448 are different hemoproteins.

TL;DR: Escherichia coli ribosomes were made deficient in a 50 S ribosomal protein component by treatment with ethanol and NH4Cl and their ability to carry out a number of partial reactions involved in polypeptide synthesis was examined.

TL;DR: EPR parameters of nitric oxide complexes of ferrous hemoproteins are not significantly affected by substitutions of two vinyl groups at positions 2 and 4 of the porphyrin ring with two ethyl groups (mesoheme) or hydrogens (deuteroheme), indicating that the nucleophilicity of porphirin side chains has no appreciable effect on the metal-ligand bond strength.

TL;DR: The structural changes in the subunit structure of human fibrinogen during plasmin digestion have been followed sequentially by examining the degraded products electrophoretically in sodium dodecyl sulfate on polyacrylamide gels in the absence or presence of mercaptoethanol as mentioned in this paper.

TL;DR: It is proposed that the spontaneous mutation rate is related to the relative rates of insertion and removal of nucleotides during synthesis of deoxyribonucleic acid.