Showing papers in "Journal of Biological Chemistry in 1974"

TL;DR: The purification of homogeneous glutathione S-transferases B and C from rat liver is described, and only transferases A and C are immunologically related.

TL;DR: It is raised that a prerequisite for the regulation of cholestero-genesis in cultured fibroblasts is the initial binding of low density lipoproteins to the high affinity surface receptor sites and that a defect in this process represents the primary genetic abnormality in the disorder familial hypercholesterolemia.

TL;DR: The determined dynamic properties of the hydrocarbon region in the lecithin liposomes differ markedly from those of the sphingomyelin liposome, which indicates that the forces which dictate the dynamic properties in the hydro carbon region in lipid bilayers predominantly originate from hydrophobic interactions.

TL;DR: The results point to a reaction between H2O2 and O2- (Haber-Weiss reaction) as a major source of the ·OH radicals and to a combined action of blocking ·OH formation as well as accelerating its removal.

TL;DR: An enzyme that hydrolyzes di-N-acetylchitobiose linkages in oligosaccharides and glycoproteins was purified to homogeneity from cultural filtrates of Streptomyces griseus and appears to consist of a single polypeptide chain.

TL;DR: Estimates of ΔGapp in the absence of denaturant suggest that several recent estimates of ΔGh2oapp are too high by 3 to 10 Cal per mole, and suggest that the mechanism of unfolding is the same in the presence of the two denaturants.

TL;DR: The sensitivity of the method for detection of changes both in soluble proteins and the insoluble membrane proteins of the cell is shown and the major membrane proteins observed and their relationship to proteins described in the literature are discussed.

TL;DR: The major envelope protein from Escherichia coli has been purified by differential heat extraction in dodecyl sulfate and subsequently freed of the detergent, and its molecular weight agrees with that derived from the mobility of the major band observed in standard dodecYL sulfate gel electrophoretic analysis of unfractionated cell envelopes after treatment at 100°.

TL;DR: In this paper, an isolated, vascularly perfused preparation of rat intestine extracted large amounts of glutamine (75 µmoles per hour), but no other amino acid, from a recirculated blood perfusate.

TL;DR: It was determined that essentially all of the O2 consumed could be accounted for by accumulation of 19-hydroxyandrostenedione and 19-oxoandrosteningione and estrogens, suggesting that the final hydroxylation is the rate-limiting step in the aromatization of androstenedion.

TL;DR: These reconstituted vesicles represent a simple model system for a biological proton pump capable of generating ATP from ADP and Pi and catalyzed light-dependent phosphorylation.

TL;DR: Fetuin isolated from fetal calf serum was shown to contain 3 carbohydrate units which are attached to serine and threonine residues on the peptide chain and are distinct from the 3 asparagine-linked heteropolysaccharides previously reported to occur in this protein.

TL;DR: The purification, by affinity chromatography, of an hepatic protein which retains the characteristic binding properties associated with the membranes is described, which indicates a high degree of aggregation in the final, water-soluble preparation.

TL;DR: The relative quantities of these peptides indicate that α1(III) chains predominate in early fetal skin, but by birth and in later lifeα1(I) chains are approximately 3 times as plentiful.

TL;DR: It was determined that steroid binding to placental microsomal cytochrome P-450 is absolutely specific for aromatase substrates, intermediates, and inhibitors, and suggest that a single species of cyto chrome P- 450 is responsible for the aromatization of both steroids.

TL;DR: It is proposed that thiols reduce nitroblue tetrazolium by at least two different pathways, one of which involves O2- and is therefore inhibitable by superoxide dismutase.

TL;DR: Evidence is presented which suggests that the hyaluronic acid-proteoglycan interactions described by Hardingham and Muir ((1972) Biochim.

TL;DR: In this article, a rat kidney γ-glutamyl transpeptidase was examined with respect to its γglutamide donor and acceptor specificities.

TL;DR: Cultured cells from a homozygote with the disorder familial hypercholesterolemia, which showed a nearly complete failure of suppression of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity by low density or very low density lipoproteins, synthesized enzyme molecules at a rate nearly 60 times greater than that of normal cells.

TL;DR: Cholesterol, which is known to reduce the fluidity of phospholipid fatty acyl chains, inhibits phosphoipid-stimulated (Na + K)-ATPase activity, and the inhibition is complete, however, only with saturatedospholipids.

TL;DR: It has been unambiguously demonstrated that the glutamic acid of the glutamylserine has been substituted by carboxylation of the γ carbon atom so that the dipeptide structure is modified and Presumably, the incorporation of the second γ-carboxyl group is dependent on vitamin K.

TL;DR: The simplest of the three isolation procedures compared is shown to yield approximately two-thirds of the total mitochondrial DNA in the tissue culture cells examined and to yield at least four times the mitochondrial DNA mass per mitochondrial volume as HeLa cells.

TL;DR: The reconstituted enzyme system was active in the hydroxylation of fatty acids, hydrocarbons, drugs, drugs (benzphetamine, hexobarbital, and ethylmorphine), and aniline and the presence of both phosphatidylcholine and deoxycholate was necessary for maximal activity.

TL;DR: It is concluded that 7-ketocholesterol may provide a powerful tool for delineation of the mechanism by which cholesterol and other steroids promote the growth of human cells in culture.

TL;DR: Endo-β-N-acetylglucosaminidase H, purified to homogeneity from cultural filtrates of Streptomyces griseus, was tested for its capacity to hydrolyze oligosaccharides from various glycoproteins.

TL;DR: It is concluded that all three components plus tropomyosin are necessary to restore full Ca2+ sensitivity, and that this reconstitution can be accomplished by combining the individual components in the absence of urea.

TL;DR: It is concluded that the binding of S17 to 16 S RNA is specific and determined the positions of S15, S16, S17, and S12 in the assembly map and have clarified subsequent interactions depending on these proteins.

TL;DR: The cell factor that is involved in tumor-associated fibrinolysis has been partially purified from the supernatant fluid of transformed chicken embryo fibroblast cultures and is an arginine-specific protease that acts as a plasminogen activator.

TL;DR: Experiments with aggregate preparations reconstituted from mixtures with [3H]acetylated or unlabeled proteoglycans showed that the larger protein fraction is derived from a portion of the protein in the proteoglycan monomer molecules while the smaller isderived from one of the small molecular weight "link proteins" that are present in proteogly can aggregate preparations.

TL;DR: Differential scanning calorimetry has been applied to the study of some simple model biomembranes and the endothermic phase transitions which they exhibit when transforming from the gel to liquid crystal phase have been studied, showing that clusters of gel and liquid crystalline lipids can coexist within this temperature range.