Showing papers in "Journal of Biological Chemistry in 1977"

TL;DR: A rapid and convenient method for peptide mapping of proteins has been developed that involves partial enzymatic proteolysis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by polyacrylamide gel electrophoresis.

TL;DR: Aurovertin, an inhibitor of oxidative phosphorylation, enhanced Pi binding via a 4-fold increase in the affinity of the enzyme for Pi (KD = 20 micronM) but did not alter binding stoichiometry.

TL;DR: The modified bovine serum albumins showed substantial changes in properties, such as solubility, electrophoretic mobility in acrylamide gel, ion exchange chromatography, and sedimentation, as compared with the unmodified protein.

TL;DR: Methoxypolyethylene glycols and catalase modified by covalent attachment of PEG-1900 to 43% of the amino groups exhibited enhanced circulating lives in the blood of acatalasemic mice during repetitive intravenous injections and no evidence of tissue or organ damage was seen.

TL;DR: Human erythropoietin, derived from urine of patients with aplastic anemia, has been purified to apparent homogeneity by seven-step procedure, which yielded a preparation with a potency of 70,400 units/mg of protein in 21% yield.

TL;DR: A protein kinase which phosphorylated histone and protamine was partially purified from bovine cerebellum and preliminary analysis suggested that the enzyme was produced from its precursor protein by a limited proteolytic reaction.

TL;DR: A potent (Na,K)-ATPase inhibitor purified from "Sigma Grade* ATP" has been identified as vanadium using electron probe microanalysis and confirmed by microwave-induced emission spectroscopy and electron paramagnetic resonanceSpectroscopy.

TL;DR: Findings support the idea that multiple basic residues, in particular arginine, on the NH,-terminal side of the phosphorylated serine act as important substrate specificity determinants for the protein kinase.

TL;DR: Two populations of mitochondria were observed upon ultrastructural examination of cardiac muscle tissue, one located directly beneath the sarcolemma (subsarcolemmal mitochondria) and another between the myofibrils (interfibrillar mitochondria).

TL;DR: This technique has compared the tryptic peptides of all the major protein components of Moloney and Rauscher leukemia viruses using only 50 to 100 microgram of total protein from each virus.

TL;DR: The D-glucose facilitated diffusion system of human erythrocytes has been studied extensively in whole cells and ghosts but has largely resisted attempts to identify the protein involved.

TL;DR: A protein kinase which phosphorylated histone and protamine was partially purified from bovine cerebellum and suggested that the enzyme was produced from its precursor protein by a limited proteolytic reaction.

TL;DR: Colony-stimulating factor, which specifically stimulates mouse bone marrow cells to proliferate in vitro and generate colonies of granulocytes, or macrophages, or both, was purified 3500-fold from mouse lung-conditioned medium, indicating that it is a glycoprotein.

TL;DR: Human thrombin generated by Taipan snake venom activation was compared with that produced by rapid thromboplastin activation and both throm bins migrated at the same rate during electrophoresis in SDS; identical pairs of NH2-terminal residues were released in three consecutive Edman degradation cycles.

TL;DR: It is demonstrated that thyroid hormones can regulate the number of cardiac beta-adrenergic receptors, and may be responsible, at least in part, for the enhanced catecholamine sensitivity of beta- adrenergic-coupled cardiac responses in the hyperthyroid state.

TL;DR: The volume of the periplasmic space in Escherichia coli and Salmonella typhimurium cells was measured, and it was shown that there is a Donnan equilibrium between theperiplasm and the extracellular fluid, and that the perplasm and cytoplasm are isoosmotic.

TL;DR: It is proposed that O2- and H2O2 cooperated in producing OH- and O2('deltag), which were the proximate causes of lipid peroxidation and of hemolysis.

TL;DR: This simple method of specifically introducing a radioactive label into cell surface sialic acids is useful in the study of cell surfaceSialic acid-containing glycoproteins.

TL;DR: Data indicate that Uracil-DNA glycosidase functions in the repair of DNA containing accidentally introduced uracil residues, and has no cofactor dependence and apparently acts by hydrolytic cleavage of the base-sugar bonds in dUMP residues.

TL;DR: The data are consistent with the hypothesis that the 140,000 Mr polypeptide is intimately involved in initial adhesion among neural cells, and represent all or parts of this cell surface molecule that were released into solution during tissue culture.

TL;DR: The results indicate that the catalytic subunit is necessary and sufficient to block progesterone-stimulated meiotic cell division, and support a model in which meioticcell division is regulated by a phosphoprotein subject to control by cAMP-dependent protein kinase.

TL;DR: Reconstitution experiments with GTP-binding fractions obtained from isoproterenol-treated membranes suggested that the characteristic synergistic amplification of hormone action by guanylnucleotides is mediated via the guanyl nucleotide binding protein.

TL;DR: It is proposed that phospholipases A2 of this general type be divided into two groups depending upon the presence or absence of distinctive structural features elucidated in this study.

TL;DR: In this article, a rat peritoneal mast cells were incubated with [35S]sulfate for less than several hours in vitro and longer incubations in vivo.