Showing papers in "Journal of Biological Chemistry in 1978"

TL;DR: The number of differences in amino acid positions between IGF-I and insulins suggests that duplication of the gene of the common ancestor of proinsulin and IGF occurred before the time of appearance of the vertebrates.

TL;DR: It is concluded that selective multiplication of the dihydrofolate reductase gene can account for the overproduction of dihydRO- in parental and methotrexate-resistant lines of L1210 murine lymphoma cells.

TL;DR: MCF-7 cells of human breast cancer origin responded to estradiol (E) treatment with increased levels of progesterone receptor (PgR) showing that human breast cells which have undergone malignant changes can continue to synthesize a specific protein under hormone control.

TL;DR: Using sequence-specific radioimmunoassays, the distribution and heterogeneity of cholecystokinin (CCK) has been determined in extracts of tissues from the central nervous system and small intestine of adult man and hog.

TL;DR: This new procedure permitted an analysis of insulin binding activity and cellular responsiveness to insulin during adipocyte conversion in vitro and found that cells appeared to be adipocytes by morphological and biochemical criteria and the specific insulin binding was 6to lo-fold greater than in undifferentiated control cells.

TL;DR: It is indicated that lysine residues are also involved in the lipoprotein-receptor interaction and may be to establish and maintain the conformation of the recognition site or the alignment of reactive residues, or both, or to chemically react through its epsilon-amino group, with the receptor (hydrogen bond formation would be such a possibility).

TL;DR: Analysis of the fractions from equilibrium density centrifugation revealed major peaks of these enzyme activities in peroxisomes and excluded contamination by mitochondria as an explanation of the results.

TL;DR: Low acyclo-Guo phosphorylating levels in cells infected with either of two herpes simplex virus mutants or with vaccinia virus correlated with low effectiveness of the compound against the virus in tissue culture.

TL;DR: It is suggested that incubation of intact fibroblasts with compactin may unmask a new regulatory system for HMG-CoA reductase that does not depend on exogenous cholesterol derived from low density lipoprotein but may depend on an endogenous compound generated from the metabolism of mevalonate.

TL;DR: Measurement of the binding of Ionomycin with Ca2+ indicates that ionomycin complexes and transports calcium ion in a one to one stoichiometry.

TL;DR: Oligodeoxyribonucleotides complementary to positions 582 to 593 of the viral DNA strand of the bacteriophage phiX174 am3 mutant were synthesized and used as specific mutagens, and induced specific mutations at high efficiency when used with heterologous template.

TL;DR: The brain FGF peptides, like pituitary FGF, are mitogenic in vitro for the same wide variety of mesoderm-derived cells and are distinguished from the platelet factor isolated from platelets and from the cationic peptide isolated from serum.

TL;DR: The interaction of vanadium in the +5 oxidation state (vanadate) with purified dog kidney Na+ and K+-stim- ulated adenosine trlphosphatase [(Na,K)-ATPase] has been studied using equilibrium binding, steady state kinetics, and measurements of relaxation kinetics.

TL;DR: A potently bactericidal cationic protein that increases the envelope permeability of susceptible Gram-negative bacteria has been isolated from human leukocytes and purified to near homogeneity and may, therefore, be a noncatalytic bactericidal protein.

TL;DR: The results indicate that the uncross-linked state of the keratins in living cells is due to the reducing intracellular environment and not to a precursor state related to the primary structure of the proteins.

TL;DR: The percentage of the total cellular carnitine associated with the mitochondria increased from 8 to 9% in nonperfused and control hearts to 25% during ischemia, indicating that a net transfer of carnitines occurred from the cytosol to the mitochondrial matrix.

TL;DR: These experiments indicate that the increase in the relative amounts of multiacetylated forms of histones H4 and H3 following butyrate treatment is the result of an inhibition of histone deacetylase activity.

TL;DR: The thermostable moiety of the enzyme appears to consist of two functional components, based upon differential thermal lability of its ability to reconstitute hormone-, NaF-, or Gpp(NH)p-stimulated adenylate cyclase activity.

TL;DR: An antibody that inhibits both human and bovine coagulation Factor V activity blocks both Xa binding to released platelets and the rapid thrombin formation associated with this binding, suggesting that Factor V from platelets is involved in the Xa-platelet interaction.

TL;DR: Results substantiate the proposal that tissue ferritins are not unique homopolymers but families of hybrid molecules consisting of different proportions of two subunit types and that both are species-specific.

TL;DR: A high molecular weight glycoprotein consisting of three disulfide-linked 142,000 molecular weight chains has been isolated from human blood platelets and yields a stable core particle of molecular weight 210,000 comprised of three 70,000 chains.

TL;DR: Accelerated phospholipid degradation and its resultant membrane dysfunction are proposed as the critical alteration that produces irreversible liver cell injury and ultimately cell death in ischemia.

TL;DR: The synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus G protein is initiated by the en bloc transfer of a high molecular weight oligosACcharide from a lipid carrier to the nascent polypeptide.

TL;DR: In this article, a new protein arylating agent 4-chloro-3,5-dinitrobenzoate reacts with e-amino groups to yield stable, negatively charged carboxydinitrophenyl lysine derivatives.