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Showing papers in "Journal of Biological Chemistry in 1979"


Journal ArticleDOI
TL;DR: Investigation of a large noncollagenous glycoprotein isolated from a mouse tumor that produces basement membrane shows that it is produced by a variety of cultured cells, suggesting that this protein or an immunologically related protein is a constituent of the basement membranes of these tissues.

2,328 citations


Journal ArticleDOI
TL;DR: The findings support the conclusion that the S-alkyl moiety of the sulfoximine binds at the enzyme site that normally binds the acceptor amino acid and increases in a manner which is parallel to those of the corresponding isosteric accepter amino acid substrates, i.e. glycine, alanine, and alpha-aminobutyrate.

1,626 citations


Journal ArticleDOI
TL;DR: Observations suggest that glutamine provides energy by aerobic oxidation from citric acid cycle metabolism, provides more than half of the cell energy when high concentrations of glucose are present, and greater than 98% when fructose or galactose is the carbohydrate.

1,161 citations


Journal ArticleDOI
TL;DR: These short chain acylglyceryl ether phosphoglycerides represent an entirely new, potent and unique class of lipid chemical mediators.

1,146 citations


Journal ArticleDOI
TL;DR: The proenzyme of a Ca2+-dependent protease-activated protein kinase previously obtained from mammalian tissues showed an ability to phosphorylate five histone fractions and muscle phosphorylase kinase, and appeared to possess multifunctional catalytic activities.

1,068 citations


Journal ArticleDOI
TL;DR: Agreement between two highly active enzyme systems in the same compartment is taken as evidence of the existence of near-equilibrium in both these systems and suggests that free cytosolic [sigma ADP] is probably 20-fold lower than measured cell ADP content in mitochondrial-containing tissues.

900 citations


Journal ArticleDOI
TL;DR: The fast spontaneous reaction between dihydrolipoamide and thioredoxin-S2 provides a mechanism for NADH or pyruvate-dependent disulfides reduction and the implication of the dithiol-disulfide oxidoreductase activity of thiOREDoxin for the regulation of enzyme activities by thiol oxidation-reduction control is discussed.

864 citations


Journal ArticleDOI
TL;DR: Relative rates of ovalbumin and conalbumin mRNA transcription were measured in isolated oviduct nuclei by allowing endogenous RNA polymerases to synthesize [32P]RNA that was then hybridized to immobilized recombinant DNA containing the respective gene sequences.

842 citations




Journal ArticleDOI
TL;DR: These studies show that these cell lines can be distinguished, regardless of growth state, by their cell-specific proteins and by their characteristic rates of synthesis of many of the shared proteins.


Journal ArticleDOI
TL;DR: Analysis of the hydration characteristics and bilayer parameters (lipid thickness, surface area/molecule) of synthetic lecithins permits an evaluation of the generalized hydration and structural behavior of this class of lipids.

Journal ArticleDOI
TL;DR: Two tetrapeptide 4-nitroanilide substrates related to the reactive site of the plasma (~1’ protease inhibitor) were studied and indicate that oxidation of the PI Met of YePI is capable to destroying its reactivity toward most serine proteases.

Journal ArticleDOI
TL;DR: A new metabolite of arachidonic acid, 5-D-(S),12- D-(R)-dihydroxy-6,8,10,14-eicosatetraenoic acid, was found upon incubation of the fatty acid with a suspension of rabbit peritoneal polymorphonuclear leukocytes collected 4 h after injection of glycogen into theperitoneal cavity.

Journal ArticleDOI
TL;DR: The recording of oxidation-reduction-related fluorescence signals of oxidized flavoprotein (Fp) and reduced pyridine nucleotide (PN) from isolated mitochondria at temperatures below -80 degrees C can be accompanished with a high degree of accuracy and a wide dynamic range and affords a foundation for the resolution of oxidation -reduction states in two and three dimensions.

Journal ArticleDOI
TL;DR: The rate of dissociation of 125I-betaNGF from the higher affinity binding site I is accelerated by unlabeled betaNGF under conditions where the occupancy is both increased and decreased.

Journal ArticleDOI
TL;DR: The brain contains two distinct molecular forms of the (Na,K)-ATPase (sodium and potassium ion-stimulated adenosine triphosphatase) that differ in at least one kinetic parameter: the affinity for the specific inhibitor strophanthidin.

Journal ArticleDOI
TL;DR: The observed equilibrium constants (Kobs) of the creatine kinase, myokinase, glucose-6-phosphatase, and fructose-1,6-diph phosphatase reactions have been determined at 38 degrees C, pH 7.0, ionic strength 0.25, and varying free magnesium concentrations.

Journal ArticleDOI
TL;DR: The present data indicate that the model prothrombinase described in this paper consists of a phospholipid-bound, stoichiometric complex of Factor Va and Factor Xa, with bound Factor Va serving as the "binding site" for Factor V, in concert with its proposed role in platelets.

Journal ArticleDOI
TL;DR: The forms of cytochrome P-450 obtained from phenobarbitaland 3-methylcholanthrene-treated rats are indistinguishable from the corresponding forms from Aroclor 1254~treated rats by all criteria examined.


Journal ArticleDOI
TL;DR: This paper showed that liver membranes prepared from livers of ethinyl estradiol-treated rats exhibit a 3 to lo-fold increase in saturable binding sites for human lz51-LDL.

Journal ArticleDOI
TL;DR: Findings suggest that one of the biochemical consequences of the binding of EGF to membranes is a rapid activation of a cyclic AMP-independent phosphorylating system.

Journal ArticleDOI
TL;DR: Arachidonic acid was incubated with rabbit peritoneal polymorphonuclear leukocytes (glycogen-induced) and compounds obtained from ether extractions were fractionated by silicic acid column chromatography to identify novel compounds.

Journal ArticleDOI
TL;DR: The adipose conversion may activate a hitherto silent gene for the stable enzyme, an important enzyme in triglyceride synthesis, if the two forms of glycerophosphate dehydrogenase are the products of independent genes.



Journal ArticleDOI
TL;DR: Structural determinants of oligosaccharides affecting interaction with the saccharide-binding sites of two lectins, the agglutinin of M, 120,000 and toxin of 82, 60,000 (RCA* and RCA~I, respectively), isolated from Ricinus communis beans have been examined by performing binding studies with iodinated glycopeptides and their degradation products.

Journal ArticleDOI
TL;DR: Iodinated glycopeptides (50 to 200 x lo3 cpm/pmol) have been utilized to examine the structural determinants affecting interaction with the saccharide binding site of concanavalin A by Scatchard plot analyses of saturation curves.