Showing papers in "Journal of Biological Chemistry in 1982"

TL;DR: Kinetic analysis indicates that TPA can substitute for diacylglycerol and greatly increases the affinity of the enzyme for Ca2+ as well as for phospholipid, and various phorbol derivatives which have been shown to be active in tumor promotion are also capable of activating this protein kinase in in vitro systems.

TL;DR: Extreme codon bias is seen for the Saccharomyces cerevisiae genes for the fermentative alcohol dehydrogenase isozyme I (ADH-I) and glyceraldehyde-3-phosphate dehydrogenased genes and a similar phenomenon is observed in the codon preferences of highly expressed genes in Escherichia coli.

TL;DR: In conclusion, catalase was inhibited by a flux of O2- generated in situ by the aerobic xanthine oxidase reaction, which provides the basis for a synergism between superoxide dismutase andCatalase.

TL;DR: This technique is quite simple, requires very small amounts of cells or tissue, and permits the simultaneous analysis of multiple samples, which should be quite useful for studies with various experimental systems of the regulation of specific mRNA levels.

TL;DR: The kinetic analysis suggested that the activation in the presence of fibrin occurs through binding of an activator molecule to the clot surface and subsequent addition of plasminogen (sequential ordered mechanism) to form a cyclic ternary complex.

TL;DR: The availability of submitochondrial fractions provides a basis for studying import of precursor polypeptides into isolated yeast mitochondria.

TL;DR: The subcellular distribution of Ca2+-activated, phospholipid-dependent protein kinase in rat brain was investigated and most of the enzyme was found to be associated with synaptosomal membranes, indistinguishable from the cytosol protein Kinase in physical, kinetic, and catalytic properties.

TL;DR: Two monoclonal antibodies produced by hybridomas obtained from a mouse immunized with a colorectal carcinoma cell line bind specifically to human gastrointestinal cancer cells.

TL;DR: In the presence of cytotoxic concentrations of menadione rapid changes in intracellular thiol and Ca2+ homeostasis were observed and were associated with alterations in the surface structure of the hepatocytes which may be an early indication of cytOToxicity.

TL;DR: A theoretical analysis of the oxidation of 3,5,3',5'-tetramethylbenzidine is presented, including a determination of the extinction coefficients and equilibrium constant for the nonradical species.

TL;DR: Results indicate that the receptor, kinase, and substrate domains are linked, possibly covalently, in epidermal growth factor receptor-kinase complex from A-431 cells.

TL;DR: A method is described by which an immunoaffinity matrix was constructed by binding antibody directly or indirectly to protein A-Sepharose 4B followed by cross-linking of the complex with dimethyl pimelimidate, which allows optimal spatial orientation of antibodies and, thus, maximum antigen binding efficiency.

TL;DR: The isolation from rabbit lung of a cofactor for thrombin-catalyzed Protein C activation is described, and the cofactor activity is destroyed by pepsin or P-mercaptoethanol.

TL;DR: Refinement has led to a revised description of the details of methotrexate binding, and a hypothetical model for substrate binding is proposed in which the pteridine ring is turned upside down while all protein and solvent atoms remain fixed.

TL;DR: The adsorption of plasma FN onto both hydrophobic and hydrophilic surfaces from serum-containing solutions was found to depend on the serum concentration, suggesting the possibility that in long term cultures, cells deposit endogenous spreading factors on top of or in place of the adsorbed non-fibronectin serum proteins.

TL;DR: It is postulate that the 220,000-dalton polypeptide is an essential component of the cap recognition complex and that its degradation in poliovirus-infected cells results in the inhibition of host cell translation.

TL;DR: Inference titration studies were carried out in order to quantify the amount of control (control strength) exerted by different steps in oxidative phosphorylation on the rate of mitochondrial oxygen uptake.

TL;DR: It is concluded that IAP, added to intact cells or isolated membranes, causes unique modification of the receptor-adenylate cyclase coupling mechanism as a result of ADP ribosylation of the Mr = 41,000 protein which is presumably one of the subunits, other than the cholera toxin substrates, of the guanine nucleotide regulatory component of the cyclase system.

TL;DR: It is significant that 5-azacytidine and 5-aza-2'-deoxycytidine act as weak inducers of erythroid differentiation of Friend eryhroleukemia cells in the same concentration range where they affect DNA methyltransferase activity.

TL;DR: A double-helical B-DNA dodecamer has been analyzed by single crystal x-ray diffraction methods and refined independently in four variants: sequence C GCGAATTCGCG at 20 degrees C and at 16 K, and CGCGAATTBrCGCG in 60% methylpentanediol at 20 and at 7 degrees C.

TL;DR: Quantitative arguments from the present work rule out a significant role of sodium in the functioning of halorhodopsin, and suggest very strongly that it is an inward directed chloride pump.

TL;DR: The ability of E-PHA and L- PHA to discriminate between Asn-linked oligosaccharides with various branching patterns can be utilized in the fractionation of these glycopeptides (see paper following).

TL;DR: These methods are used to analyze and inventory the nucleotide content of Salmonella typhimurium in balanced log phase growth and developed a set of enzymatic and chemical methods to be used in conjunction with the chromatographic separations for verifying the identity of nucleotide and characterizing novel nucleotides.

TL;DR: Peptide mapping by sodium dodecyl sulfate-polyacrylamide gel electrophoresis following treatment with papain, chymotrypsin, or Staphylococcus aureus V8 protease and by high performance liquid chromatography following trypsinolysis indicates that form 3a is a unique gene product.

TL;DR: The amino acid sequence of the protein as determined from the nucleotide sequence disagrees with the published alcohol dehydrogenase isozyme I (ADH-I) sequence for 5 of the 347 amino acid residues.

TL;DR: There is no reversal of activation or binding under activating conditions and the rate constant for activation is unchanged from 10 mi to 100 PM GTPyS, so reversal of binding and activation, which occurs only in the absence of divalent cation, is not a first order process.