Showing papers in "Journal of Biological Chemistry in 1983"

TL;DR: The results show that platelets contain a type beta transforming growth factor, which is distinct from platelet-derived growth factor and elicits 50% of its maximal biological response at concentrations less than 5 x 10(-12) M.

TL;DR: Observations indicate that the function of E2 is the transfer of activated ubiquitin to the site of conjugation in the form of an E2-ubiquitin thiol ester intermediate, which is consistent with the notion that a covalent linkage is formed between E2 and Sepharose-bound ubiquitIn.

TL;DR: It is suggestive that protein kinase C is a phorbol ester-receptive protein, and the results presented seem to provide clues for clarifying the mechanism of tumor promotion.

TL;DR: Experiments with the synthetic diacylglycerol and Ca2+ ionophore A23187 suggested that the proteinosphorylation catalyzed by protein kinase C was a prerequisite requirement for the release of serotonin, and that the receptor-linked protein phosphorylation and Ca1+ mobilization acted synergistically to elicit the full physiological cellular response.

TL;DR: Human diferric transferrin binds to the surface of K562 cells, a human leukemic cell line, and the loss of surface receptors upon internalization never exceeds 60-70% of the initial binding capacity suggests that receptors enter the cell with ligand but are replaced so as to maintain a constant, albeit reduced, receptor number on the cell surface.

TL;DR: It is noted that Ca2+ protease II, which is active at higher concentrations of Ca2+, proteolytically activates protein kinase C irrespective of the presence and absence of phospholipid and diacylglycerol.

TL;DR: Equilibrium binding studies and photoaffinity labeling experiments demonstrate that the 41,000-dalton subunit of the IAP substrate has a specific binding site for guanine nucleotides.

TL;DR: A naturally occurring inducer of terminal differentiation in a murine myelomonocytic leukemia cell line (WEHI-3B) was purified to apparent homogeneity from medium conditioned by lungs from mice injected with bacterial endotoxin to reveal a single protein band coincident with both differentiation-inducing and granulocyte colony-stimulating activity.

TL;DR: Detailed characterization of the cleavage products has revealed that topoisomerase II cuts DNA with a four-base stagger and is covalently linked to the protruding 5'-phosphoryl ends of each broken DNA strand.

TL;DR: It is concluded that the low pH in endocytic vesicles is essential for the dissociation of iron from transferrin and its delivery to the cell, but is not required for recycling of transferrin, and presumably of its receptor.

TL;DR: The results indicate that the concentration at which bile salt aggregation occurs varies widely and is determined not only by the number, type, and orientation of nuclear substituents, but also by side chain structure.

TL;DR: The perhydroxyl radical (HO2) reaction with linoleic, linolenic, and arachidonic acids has been studied in aqueous ethanolic solutions by the stopped flow technique.

TL;DR: IAP treatment of NG108-15 cells caused specific uncoupling of negative signal transduction from inhibitory receptors to the adenylate cyclase catalytic unit via the guanine nucleotide regulatory protein, as a result of ADP-ribosylation of one of the subunits of the regulatory protein.

TL;DR: The data indicate that despite the limited sequence homology between eTGF and EGF, these two ligands exhibit a remarkably similar mode of interaction with a common eT GF/EGF membrane receptor type.

TL;DR: It was found that the number of Gal residues/cluster and their branching mode are major determinants of binding affinity of ligands to the hepatic lectin on the surface of hepatocytes.

TL;DR: Findings strongly suggest that the T beta gamma subunit is an activator of the GTPase activity to enable the T alpha subunit to interact with rhodopsin effectively.

TL;DR: The time course of mRNA accumulation was different for each of these mRNAs, indicating they do not respond synchronously during differentiation, and a recombinant bacterial library containing cDNA prepared from mRNA of adipose 3T3 cells is constructed.

TL;DR: The abundance of this calmodulin-dependent protein kinase indicates that its activation is likely to be an important biochemical response to increases in calcium ion concentration in neuronal tissue.

TL;DR: The results suggest that the apoprotein of lipoprotein [a] is composed of two subunits which are similar in molecular weight and are held together by one or more disulfide bonds.

TL;DR: Phosphatidylinositol metabolism and lactate production are stimulated in mouse thymocytes and pig lymphocytes within 1 h of loading with quin 2 and significant increases in RNA synthesis occur after 8 h and the possibility that quin 1 itself affects the concentration of free Ca2+ or other cations in the cells is left open.

TL;DR: Biochemical properties of the heat shock or stress proteins of mammalian cells have been investigated using two-dimensional gel electrophoresis and immunological techniques and two of the stress proteins, the 80- and 90-kDa species, were found to be phosphoproteins in all cell types examined.

TL;DR: It is suggested that HCII is the only thrombin inhibitor in human plasma that can be activated by dermatan sulfate.

TL;DR: Three monoclonal antibodies were prepared by fusing FO myeloma cells with spleen cells from a mouse immunized with a partially purified preparation of insulin receptors from human placenta to immunoprecipitate insulin and somatomedin-C receptors from solubilized human lymphoid cells and human Placenta membranes that had been 125I-labeled with lactoperoxidase.

TL;DR: It is concluded that the hormonally specific compartmentation of cyclic AMP and cAMP-dependent protein kinase that occurs in intact heart is not explained on the basis of cellular heterogeneity but has a subcellular basis within the cardiomyocyte.

TL;DR: Results suggest that the interaction of the dye with anionic sites within these Ca2+-binding proteins produces the dye-protein complex which absorbs at 600-615 nm, which will be useful in their identification and purification from various tissues.

TL;DR: A reconstituted reticulocyte translation system originally designed to be deficient in eukaryotic initiation factor 4B was used to identify a new activity required for maximal synthesis of rabbit globin that purifies as a stable, high molecular weight complex by a variety of chromatographic procedures and is termed eIF-4F.

TL;DR: The identification of specific steroid metabolites as diagnostic of particular P-450 isozymes should be useful for the assessment of isozymic contributions to microsomal activities and, in addition, facilitate comparisons of P- 450 isoz enzymes isolated in different laboratories.

TL;DR: The conclusion is that the sole site of IAP action is the guanine nucleotide regulatory protein (Ni) that is required for transmission of inhibitory signals from receptors to the catalytic unit of adenylate cyclase; the function of Ni is lost upon IAP-catalyzed ADP ribosylation of the Mr = 41,000 protein which appears to be an active subunit of Ni.

TL;DR: The 1/20/5-D-4 monoclonal antibody appears to recognize a common determinant in their polysaccharide moieties, consistent with several biochemical analyses showing the absence of keratan sulfate in proteoglycan synthesised by this tissue.

TL;DR: It is shown that Sertoli cells in the seminiferous tubule are one potential source, since primary cultures of rat Sertolis cells secrete a protein having the same immunochemical and physical properties as clusterin isolated from ram rete testis fluid, which binds to concanavalin A-Sepharose and also to wheat germ agglutinin Sepharose.