Showing papers in "Journal of Biological Chemistry in 1984"

TL;DR: The alpha subunit of GO was isolated without the use of ligands known to dissociate other G proteins, and should be of great utility in elucidating the mechanism of action of this family of GTP-binding proteins.

TL;DR: The agreement between the in vivo and in vitro studies suggests that mammalian DNA topoisomerase II is a drug target in vivo, and the cytotoxic action of epipodophyllotoxins may be analogous to the bactericidal action of nalidixic acid.

TL;DR: Dramatic differences in the amounts and distribution of terminal GlcNAc residues on phenotypically different lymphocyte populations are described, but the presence of a novel protein-saccharide linkage, which is present on numerous lymphocyte proteins, is described.

TL;DR: The hypothesis that iron-catalyzed formation of hydroxyl radical from superoxide anion radical (O-.2) and H2O2 requires the availability of at least one iron coordination site that is open or occupied by a readily dissociable ligand such as water is investigated.

TL;DR: Partially purified microtubule protein contains a kinase that can rephosphorylate tau, which promoted significantly more rapid and more extensive polymerization of microtubules though there was no obvious difference in the micro Tubulin formed.

TL;DR: Two factors are identified which may account for the ability of the enzyme to stabilize high-oxidation states of the heme iron during catalysis: 1) the proximal histidine forms a hydrogen bond with a buried aspartic acid side chain, Asp-235; and 2) the heME environment is more polar than in the cytochromes c or globins.

TL;DR: Results indicate that, in A431 cells exposed to tumor promoters, C-kinase catalyzes phosphorylation of a significant population of EGF receptor molecules, which results in decreased self-phosphorylated EGF receptors both in vivo and in vitro and in decreased EGF-stimulated tyrosine kinase activity in vivo.

TL;DR: A role for particulate guanylate cyclase in the molecular mechanisms underlying the physiological effects of atrial natriuretic factor such as vascular relaxation, natriuresis, and diuresis is suggested.

TL;DR: The precise temporal expression of these MHC genes in correlation with the myosin phenotype both during cardiac development and in response to different thyroid hormone levels are established and also document their expression in other muscle tissues.

TL;DR: The gene coding for protein A from Staphylococcus aureus has been isolated by molecular cloning, and a subclone containing an 1.8-kilobase insert was found to give a functional protein A in Escherichia coli.

TL;DR: Free hemoglobin may be biologically hazardous, in part because it acts as a "Fenton" reagent, having the potential to catalyze hydroxyl-radical generation in areas of inflammation.

TL;DR: It was concluded that vitamin E trapped the peroxy radical and the resulting alpha-chromanoxy radical reacted with vitamin C to regenerate vitamin E.

TL;DR: Endo-beta-N-acetylglucosaminidase F preparations from Flavobacterium meningosepticum have been found to contain peptide:N-glycosidase activity, which readily cleaves the beta-aspartylglycosylamine linkage of a fetuin triantennary complex glycopeptide.

TL;DR: It is found that in bovine cerebral cortex there are three proteins of similar molecular weight which are modified by pertussis toxin which differ in two functions: susceptibility to ADP-ribosylation and GTPase activity.

TL;DR: In this paper, the ability of polyheptapeptides to form two-stranded alpha-helical coiled-coils in benign medium was monitored by molecular weight determinations and circular dichroism studies and their physical properties were compared to carboxamidomethylated alpha-tropomyosin at cysteine 190.

TL;DR: Biochemical fractionation of cells grown under heat shock showed that following its synthesis a portion of the 72,000-Da protein (and its isoforms) becomes associated with the nucleus while some remains in the cytoplasm.

TL;DR: Cell-free translation of rat striatal mRNA selected by hybridization with pRPE2 DNA resulted in the synthesis of a 31,000-Da protein, consistent with a single preproenkephalin gene.

TL;DR: The results demonstrate that the NH2-terminal formyl peptides produced by E. coli, of which formyl-methionyl-leucyl-phenylalanine appears to be the major component, are the peptide mediators responsible for leukocyte chemotactic activity in the bacterial culture extracts.

TL;DR: The level of gangliosides GM1 and GM3 in membranes may modulate PDGF receptor function by affecting the degree of tyrosine phosphorylation and may alter the affinity of the receptor for PDGF.

TL;DR: It was determined that all of the Ca2+ released by Ins(1,4,5)P3 originated from non-mitochondrial, vesicular stores and reaccumulation was associated with dephosphorylation of this compound.

TL;DR: A nerve growth factor (NGF) receptor interactive monoclonal antibody (192-IgG) which enhances beta-NGF binding to PC12 cells has been produced and implicate the slow receptor as the mediator of the biological response.

TL;DR: The variation in A-I/A-II ratio of HDL density subfractions therefore reflects different proportions of two discrete types of particles: particles containing A-i and A-II in a nearly constant ratio and particles containing C, D, and E, and LCAT (EC 2.3.43) in Lp (A-I with A- II) particles.

TL;DR: The physical characteristics of Gi are important determinants of its role as the inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase.

TL;DR: Four mouse monoclonal antibodies specific for human epidermal growth factor (EGF) receptors have been prepared using EGF receptor protein from human A431 epidermoid carcinoma cells as immunogen and the effect of these antibodies on two known functions of the E GF receptor: EGF binding and tyrosine kinase is determined.

TL;DR: A monoclonal antibody, named S12, is developed, which demonstrates dramatically enhanced binding to platelets after thrombin activation and suggests that S12 may serve as a useful probe of in vivo platelet activation.

TL;DR: A procedure has been developed for the iodination of human transforming growth factor-beta (TGF-beta) with full retention of biological activity using the iodinated peptide, saturable receptors have been found on normal rat kidney fibroblasts, a cell line that will grow in soft agar in the presence of TGFs but not in their absence.

TL;DR: Results indicate that cultured bovine aortic endothelial cells synthesize and secrete a protein which inhibits plasminogen activators and is distinct from protease nexin, a major endothelial cell product, and, as such, probably plays an important role in regulating the fibrinolytic system of these cells.

TL;DR: Results indicate that platelet secretion is associated with the expression of an Mr = 140,000 integral membrane protein composed of a single polypeptide chain which may be component of the internal granule membrane which is fused with the plasma membrane during activation.

TL;DR: The magnitude and kinetics of the response suggest that the primary action of insulin in the regulation of P-enolpyruvate carboxykinase synthesis is exerted at the level of mRNAPEPCK transcription.

TL;DR: Observations show that nonenzymatic glycosylation induces a conformational change in human serum albumin, and differences in affinity suggest that lysine 525 plays a key role in the binding of physiologically important ligands to albumin.