Showing papers in "Journal of Biological Chemistry in 1985"

TL;DR: A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+ using an 8-coordinate tetracarboxylate chelating site with stilbene chromophores that offer up to 30-fold brighter fluorescence.

TL;DR: It is proposed that camptothecin blocks the rejoining step of the breakage-reunion reaction of mammalian DNA topoisomerase I, which results in the accumulation of a cleavable complex which resembles the transient intermediate proposed for eukaryotic DNATopoisomersase I.

TL;DR: Human tumor necrosis factor was purified to homogeneity from serum-free tissue culture supernatants of the HL-60 promyelocytic leukemia cell line induced by 4 beta-phorbol 12-myristate 13-acetate.

TL;DR: A murine monoclonal anti-platelet antibody is developed and characterized that binds to activated platelets, but not to unstimulated platelets and inhibits fibrinogen-mediated platelet aggregation.

TL;DR: The results suggest that c-fos, c-myc, and actin induction may be general nuclear responses to growth or differentiation factors in a variety of different cell types.

TL;DR: The crystal structure of Pseudomonas putida cytochrome P-450cam in the ferric, camphor bound form has been determined and partially refined to R = 0.23 at 2.6 A.

TL;DR: Observations suggest that the mechanisms of replication, transcription, processing, and translation in mitochondria are highly conserved throughout higher vertebrates.

TL;DR: The purified enzyme was inhibited by EDTA and 1,10-phenanthroline but not by serine or thiol proteinase inhibitors, suggesting that the enzyme is a metalloendoproteinase.

TL;DR: Analysis of treated and untreated conditioned medium by gel filtration revealed that the latent and active PAIs migrated with apparent Mr values of 30,000 and 50,000, respectively, which indicate that the two PAIs are immunologically related and suggest thatThe latent form is converted into the active form by the sodium dodecyl sulfate present during the purification.

TL;DR: Data suggest that ferritin may function in vivo as a source of iron for promotion of superoxide-dependent lipid peroxidation, and that initiation is not via an iron-catalyzed Haber-Weiss reaction.

TL;DR: Oligonucleotide probes with deoxyinosine residues at ambiguous points seem to be useful as hybridization probes for cloning genes for proteins containing amino acids with degenerate codons.

TL;DR: The results support the transporter translocation model for insulin-activated hexose transport and implicate vicinal sulfhydryl groups in a post-insulin binding event essential for the translocation of glucose transporters to the plasma membrane.

TL;DR: Purified stromelysin degraded casein, cartilage proteoglycans, fibronectin, alpha 1-proteinase inhibitor, and immunoglobulin G2a and had limited activity on laminin, elastin, type IV collagen, and gelatin, but did not degrade type I collagen.

TL;DR: It is shown that the 90-kDa receptor-associated phosphoprotein is an abundant cytosolic protein that reacts with a monoclonal antibody that recognizes the 90 -kDa phosphop protein that binds steroid receptors in the chicken oviduct.

TL;DR: It is demonstrated that 1,25-(OH)2D3 can inhibit DNA synthesis and cell proliferation of the G-8 myoblast cells in a dose-dependent manner and the possibility that muscle is a target tissue for 1, 25-dihydroxyvitamin D3 and the hormone may act to initiate terminal differentiation of myOBlast cells is supported.

TL;DR: The intracellularly trappable fluorescent Ca2+ indicator quin-2 was used to measure free cytosolic Ca2+, [Ca2+]i, in the two highly dedifferentiated tumor cell lines, Ehrlich and Yoshida ascites carcinomas, and it was found that endogenous heavy metals are responsible for partially quenching quin2 fluorescence trapped inside the cells.

TL;DR: It is proposed that the alpha-subunit of Ni, or a like protein, mediates signal transduction arising from Ca-mobilizing receptors, probably prior to Ca2+ gating.

TL;DR: A mixed micellar assay for protein kinase C was developed to investigate the specificity and stoichiometry of activation by phospholipids and diacylglycerols and establishes that a phospholIPid bilayer is not required for protein Kinase C activation and that activation of monomeric protein kinases C occurs.

TL;DR: The data suggest that a region near the carboxyl-terminus of the alpha-chain of fibrinogen interacts with the fibrInogen receptor on activated platelets, and support the concept that the sequence Arg-Gly-Asp-Ser has been conserved for use in a variety of cellular adhesive processes.

TL;DR: The substrate specificity of protein kinase C was studied and compared with that of cyclic AMP-dependent protein kinases by using bovine brain myelin basic protein as a model substrate to provide some clues to understanding the rationale that these kinases may show different but sometimes similar functions depending on the structure of target phosphate acceptor proteins.

TL;DR: It is concluded that Cys 347 and not Asn 343 or Asp 346 is the site of pertusis toxin-catalyzed ADP-ribosylation in transducin.

TL;DR: The results suggest that acetaminophen and N-acetyl-p-benzoquinone imine exert their cytotoxic effects via a disruption of Ca2+ homeostasis secondary to the depletion of soluble and protein-bound thiols and may prove to be of general applicability to a variety of hepatotoxins.

TL;DR: Results show that the bicarbonate-sensitive adenylate cyclase system regulates sperm motility and suggest that this system is common to all mammals.

TL;DR: A specific contractile property, velocity of shortening, and protein subunit composition of single fibers from adult rabbit soleus muscles are characterized, suggesting that some myosin molecules consist of mixtures of slow- and fast-type subunits.

TL;DR: A new gene function involved in the expression of mitochondrial cytochrome b is described, which is confirmed by analysis of the mitochondrial translation products in E158, a respiratory deficient strain of Saccharomyces cerevisiae with a recessive mutation in nuclear DNA.

TL;DR: Using the major peptide (GIIIA) in electrophysiological studies on nerve-muscle preparations and in single channel studies using planar lipid bilayers, it is established that the toxin blocks muscle sodium channels, while having no discernible effect on nerve or brain sodium channels.

TL;DR: The COOH terminus of the externally disposed variant surface glycoprotein (VSG) of the eukaryotic pathogenic protozoan Trypanosoma brucei strain 427 variant MITat 1.4 (117) is covalently linked to a novel phosphatidylinositol-containing glycolipid.

TL;DR: Quantitative studies of MCF-7 cells and CV-1 cells using the permeant cationic compound tetraphenylphosphonium, in conjunction with fluorescence microscopy using rhodamine 123 (Rh123), indicate that the mitochondrial and plasma membrane potentials affect both uptake and retention of these compounds.

TL;DR: It is demonstrated that estrogen can regulate acutely the levels of EGF receptor in vivo and raise the possibility that events coupled to this receptor may play a role in estrogen-stimulated growth.

TL;DR: The uptake of high-density lipoprotein (HDL)-associated apolipoprotein A-I and cholesterol esters was estimated in 16 tissues of the rat using rat HDL doubly labeled with nondegradable tracers; covalently attached 125I-tyramine-cellobiose traced apo-A-I, and [3H]cholesteryl linoleyl ether traced cholesterol ester.