Showing papers in "Journal of Biological Chemistry in 1989"

TL;DR: A new group of fluorescent indicators with visible excitation and emission wavelengths has been synthesized for measurements of cytosolic free Ca2+.

TL;DR: The structure of the sterol 26-hydroxylase cDNA reveals it to be a mitochondrial cytochrome P-450, and blotting experiments revealed that the mRNA for this enzyme is expressed in many tissues and that it is encoded by a low copy number gene in the rabbit genome.

TL;DR: The complete structure of this enzyme is presented along with the evidence that it is identical to the 92-kDa metalloprotease secreted by normal human alveolar macrophages, phorbol ester-differentiated monocytic leukemia U937 cells, fibrosarcoma HT1080 cells, and cultured human keratinocytes.

TL;DR: The unexpected discovery of pentose-mediated protein cross-linking raises new questions concerning the aging process and suggests ribose or ribonucleotide metabolites as precursors.

TL;DR: This minireview has attempted to provide some overall perspective on the question of how various forms of diffusion in reduced dimensions, or diffusion within a nonspecifically bound state, can speed biological interactions beyond the limits normally set by three-dimensional diffusion processes.

TL;DR: The amino acid sequence demonstrates that this protein shares significant homology with human TIMP, including conservation of the positions of the 12 cysteine residues and 3 of 4 tryptophan residues, and the name TIMP-2 is proposed for this inhibitor.

TL;DR: The mechanisms for activation of Ca2+ entry by thapsigargin and methacholine are the same and are consistent with the idea that entry is initiated by the depletion of the intracellular inositol 1,4,5-trisphosphate-sensitive Ca2+, according to previously proposed models.

TL;DR: It is demonstrated that fluo-3 can be loaded into fibroblasts and lymphocytes by incubation with the pentaacetoxymethyl ester of the dye and that the ester is hydrolyzed intracellularly to yield genuine flui-3 capable of indicating changes in [Ca2+]i induced by agonist stimulation.

TL;DR: It is found that at low ATP concentrations (0.5-2 microM) the inhibition of ATPase activity was essentially complete at a CPA concentration of 6-8 nmol/mg protein, indicating stoichiometric reaction of CPA with the Ca2+-ATPase, which suggests that CPA interferes with the ATP-induced conformational changes related to Ca2- transport.

TL;DR: In this article, it was shown that the phi 29 DNA polymerase is the only enzyme required for efficient DNA replication with the initiation primer as the only additional protein requirement, and that conditions that increase the stability of secondary structure in the template do not affect the processivity and strand displacement ability of the enzyme.

TL;DR: The derived protein sequence of PG I showed sufficient homology with the PG II sequence to strongly suggest that the two proteins were the result of a gene duplication, and it is suggested that PG I be called biglycan.

TL;DR: Findings suggest that cyclosporin A essentially titrates a mitochondrial component which is present at 80-90 pmol/mg protein, and it is proposed that this component is a solute unselective, regulated pore or a factor involved in controlling such a structure.

TL;DR: The activity for this unusual oxidation reaction which is contained in the 100,000 x g supernatant was lost after desalting on a Sephadex G-25 column and a small molecule cofactor was found to be required for the restoration of activity.

TL;DR: In this article, the authors examined the effect of transforming growth factor-beta 1 on the expression of the very late antigens or alpha beta 1 group of integrins in human cell lines.

TL;DR: A human non-pancreatic phospholipase A2 that is present in platelets and is enriched in rheumatoid synovial fluid is purified and has a half-cystine pattern that is characteristic of the snake venom group II enzymes.

TL;DR: The data suggest that stimulated secretion of vWF from endothelial cells entails fusion ofvWF-containing storage granules with the plasma membrane, and GMP-140 is subsequently removed from the endothelial surface, most likely by an endocytic mechanism.

TL;DR: The effects of TGF-beta on procollagenase, 72-kDa progelatinase, TIMP, and PAI-1 were selective and showed temporal differences, indicating that the regulation of TIMP mRNA and protein levels may be a secondary response to T GF-beta.

TL;DR: By testing smaller sequences within the 19-mer peptide, a constituent pentapeptide, IKVAV (Ile-Lys-Val-Ala-Val), was identified as the active site for cell adhesion and neurite outgrowth, suggesting that this sequence is one of the principle sites in laminin which regulate cellular behavior.

TL;DR: The existence of a "sphingomyelin cycle" in human cells is demonstrated and it is demonstrated that such sphingolipid cycles may function in a signal transduction pathway and in cellular differentiation.

TL;DR: Fluorescent indicators sensitive to cytosolic concentrations of free Na+ have been synthesized and characterized, resulting in effective dissociation constants for Na+ of about 20 mM against a background of 120 mM K+.

TL;DR: Evidence is presented that high affinity binding of PDGF requires association of two different receptor subunits: an alpha-subunit that can bind either a B- or an A-chain ofPDGF, and a beta-sub unit that can binds only a B/A-chain.

TL;DR: Data indicate that the exposure of the platelet factor Va receptor is directly coupled to plasma membrane vesiculation and that this event can be dissociated from other activation-dependent platelet responses.

TL;DR: The tod-C1C2BAD genes show significant homology to the reported nucleotide sequence for benzene dioxygenase and cis-1,2-dihydroxycyclohexa-3,5-diene dehydrogenase from P. putida 136R-3.

TL;DR: The role of the heliiturn-helix in recognition of sequence-specific binding sites on DNA will be evaluated in light of the recently defined repressor-operator complexes.