Showing papers in "Journal of Biological Chemistry in 1994"

TL;DR: One such compound, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one, LY294002, completely and specifically abolished PtdIns 3-kinase activity, which may be beneficial in the treatment of proliferative diseases as well as in elucidating the biological role of the kinase in cellular proliferation and growth factor response.

TL;DR: Reporter constructs containing truncated promoter regions revealed that activation of NF-kappa B/Rel is critical in the induction of iNOS by LPS, however, additional, newly synthesized proteins contribute to the NF- kappa Bd-dependent transcription factor complex on the iN OS promoter in LPS-treated mouse macrophages.

TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.

TL;DR: In this article, the role of HIF-1 as a mediator of adaptive responses to hypoxia that underlie cellular and systemic oxygen homeostasis was investigated in Hep3B cells.

TL;DR: Neither the receptor-associated activity of phosphatidylinositol 3'-kinase nor tyrosine phosphorylation of phospholipase C-gamma were affected by stimulation of Flt1 or KDR expressing cells, and phosphorylated of GTPase activating protein was only slightly increased.

TL;DR: The studies reported here examine the effects of some systematic chemical structural changes in both the cationic lipid components and the neutral phospholipid requirements for transfection activity in COS.

TL;DR: In this paper, the influence of NO on membrane lipid peroxidation induced by O2-, H2O2, and OH derived from xanthine oxidase (XO) and by ONOO- was assessed by formation of thiobarbituric acid-reactive products and by liquid chromatography-mass spectrometry.

TL;DR: Data indicate that interaction of AGEs with cellular targets, such as ECs, leads to oxidant stress resulting in changes in gene expression and other cellular properties, potentially contributing to the development of vascular lesions.

TL;DR: The cloned gene encodes a 32-kDa putative cysteine protease (CPP32) with significant homology to Caenorhabditis elegans cell death protein Ced-3, mammalian interleukin-1 beta-converting enzyme (ICE), and the product of the mouse nedd2 gene, which is highly expressed and most abundant in cell lines of lymphocytic origin.

TL;DR: The impairment of microbial activity by the cyclic hydrocarbons most likely results from hydrophobic interaction with the membrane, which affects the functioning of the membrane and membrane-embedded proteins.

TL;DR: Purified PIGF isoforms had little or no direct mitogenic or permeability-enhancing activity, but were able to significantly potentiate the action of low concentrations of VEGF in vitro and, more strikingly, in vivo.

TL;DR: It is demonstrated for the first time that expression of the protein in MA-10 cells in the absence of hormone stimulation is sufficient to induce steroid production and it is proposed that this protein is required in the acute regulation of steroidogenesis.

TL;DR: It is hypothesized that the internalization of cell surface amyloid precursor protein (beta PP) via coated pit-mediated endocytosis is one pathway leading to A beta generation and release into medium.

TL;DR: It is reported that the principal defect in poor metabolizers is a single base pair (G-->A) mutation in exon 5 of CYP2C19, which creates an aberrant splice site that results in a truncated, non-functional protein.

TL;DR: It is concluded that activation of wortmannin-sensitive PI 3-kinase plays a pivotal role in the intracellular signaling pathways arising from the insulin receptor autophosphorylation and leading to certain metabolic responses.

TL;DR: The Saccharomyces cerevisiae thioredoxin reductase gene was also cloned and sequenced, and the deduced amino sequence was shown to be 51% identical with that of the Escherichia coli enzyme.

TL;DR: It is concluded that the biosynthetic release of LTC4 from cells is mediated by the 190-kDa product of the MRP gene, a primary-active ATP-dependent export pump for conjugates of lipophilic compounds with glutathione and several other anionic residues.

TL;DR: SR-BI and CD36 define a second class of scavenger receptors, designated class B, whose predicted protein sequence of 509 amino acids is approximately 30% identical to those of the four previously identified family members.

TL;DR: A useful approach to understanding the functions of a cellular component such as GSH is to remove it and to determine the consequences of its deletion.

TL;DR: The results imply that jasplakinolide may exert its cytotoxic effect in vivo by inducing actin polymerization and/or stabilizing pre-existing actin filaments.

TL;DR: A cDNA coding for a new human matrix metalloproteinase (MMP) has been cloned from a cDNA library derived from a breast tumor, providing support to the hypothesis that the isolated cDNA codes for an authentic collagenase.

TL;DR: Ubiquitin-mediated proteolysis provides an important mechanism for regulating a variety of cellular processes and proteins conjugated to polymers of ubiquitin may be selected for degradation by a single subunit of the 26 S protease complex.

TL;DR: The heregulin/neu differentiation factor gene products were purified and cloned based on their ability to stimulate the phosphorylation of a 185-kDa protein in human breast carcinoma cell lines known to express erbB2, indicating that other components besides erBB2 may be required forHeregulin binding.

TL;DR: Clearly the reactivity of the reduced flavin with 0, is modulated by the protein environment in which i t is located, both in terms of rates and products of the reaction.