Showing papers in "Journal of Biological Chemistry in 1995"

TL;DR: It is reported that thiazolidinediones are potent and selective activators of peroxisome proliferator-activated receptor γ (PPARγ), a member of the nuclear receptor superfamily recently shown to function in adipogenesis, and raised the intriguing possibility that PPARγ is a target for the therapeutic actions of this class of compounds.

TL;DR: The results indicate that the activation of Raf is suppressed and that its inactivation is accelerated by a downstream component(s) of the MAP kinase pathway.

TL;DR: A novel 30-kDa secretory protein, Acrp30 (adipocyte complement-related protein of 30 kDa), that is made exclusively in adipocytes and whose mRNA is induced over 100-fold during adipocyte differentiation is described.

TL;DR: The specific roles of three MAPKs, namely ERK, JNK and FRK, in modulation of both the level and activity of AP-1, are discussed.

TL;DR: It is demonstrated that p38, like JNK, is activated by treatment of cells with pro-inflammatory cytokines and environmental stress and the mechanism of p38 activation is mediated by dual phosphorylation on Thr-180 and Tyr-182.

TL;DR: The biochemical purification of HIF-1 from Epo-producing Hep3B cells and non-Epo-producing HeLa S3 cells concludes that in both cobalt chloride-treated HeLa cells and hypoxic Hep3 B cells HIF -1 is composed of two different subunits: 120-kDa Hif-1α and 91-94-k da HIF,1β.

TL;DR: Multiple receptor pathways feeding into multiple lipid pathways have the common end result of activating protein kinase C by production of its second messenger, diacylglycerol.

TL;DR: Activation of 72T4Cl on the cell membrane provides a basic mechanism for spatially regulated extracellular proteolysis and presents a new target for prognosis and treatment of metastatic disease.

TL;DR: Overproduction of the siderophore and its transport system at low iron is in this species well established to be the result of negative transcriptional repression, but the detailed mechanism may be positive in other organisms.

TL;DR: Specific vacA genotypes of H. pylori strains are associated with the level of in vitro cytotoxin activity as well as clinical consequences, which implies that recombination has occurred in vivo between vacA alleles.

TL;DR: The results indicate that endocytosis was the major mechanism of entry in cationic lipid-mediated gene transfer and suggest that attention to specific steps in the cellular process may further improve the efficiency of transfection and increase its use in a number of applications.

TL;DR: It is demonstrated that curcumin (diferuloylmethane), a known anti-inflammatory and anticarcinogenic agent, is a potent inhibitor of NF-κB activation.

TL;DR: A functional role of the ELR motif is suggested in determining the angiogenic or angiostatic potential of CXC chemokines, supporting the hypothesis that the net biological balance between angiogenicity and angiOSTatic CXCs may play an important role in regulating overall angiogenesis.

TL;DR: The receptor for advanced glycation end products (RAGE), a newly-identified member of the immunoglobulin superfamily, mediates interactions of AGE-modified proteins with endothelium and other cell types.

TL;DR: A 28-base pair element in the 5′ promoter that mediates hypoxia-inducible transcription in transient expression assays is identified and sequence motifs in the 3′-untranslated region that may mediate VEGF mRNA stability are revealed.

TL;DR: Induced expression of the cloned protein results in ligand-independent triggering of cytotoxicity, suggesting that it is involved in cell death induction by Fas/APO1.

TL;DR: The results suggest that cell-permeable synthetic peptides carrying a functional cargo can be applied to control signal transduction-dependent subcellular traffic of transcription factors mediating the cellular responses to different agonists and can be used to study other intracellular processes involving proteins with functionally distinct domains.

TL;DR: It is reported that both human and chicken MMP-2, free of tissue inhibitors of metalloproteinases (TIMPs) are capable of cleaving soluble, triple helical type I collagen generating the ¾- and ¼-length collagen fragments characteristic of vertebrate interstitial collagenases.

TL;DR: Data indicate that the MAP kinase pathway is absolutely required for NGF-induced neuronal differentiation in PC-12 cells.

TL;DR: The very high efficiency of cyclodextrins in stimulating cell cholesterol efflux suggests that these compounds can be used in two general ways for studies of atherosclerosis: 1) as research tools to probe mechanisms of cholesterol transport and aspects of membrane structure or 2) as potential pharmacological agents that could modify in vivo cholesterol metabolism and influence the development of the atherosclerotic plaque.

TL;DR: The results strongly suggest that the FKBP12-rapamycin complex interacts with homologous ligands in yeast and mammalian cells and that the loss of mTOR function is directly related to the inhibitory effect of rapamycin on G1- to S-phase progression in T-lymphocytes and other sensitive cell types.

TL;DR: It is demonstrated that transforming mutants of p21ras induce the cyclin D1 promoter in human trophoblasts, mink lung epithelial cells, and in Chinese hamster ovary fibroblast cell lines, providing evidence for cross-talk between the p 21ras and cell cycle regulatory pathways.

TL;DR: It is shown that a Lys Arg conversion at either position 29 or position 48 in the fusion's Ub moiety greatly reduces ubiquitination and degradation of Ub fusions to β-galactosidase and that structurally different multi-Ub chains have distinct functions in Ub-dependent protein degradation.

TL;DR: In normal mice, elevated VEGF mRNA levels during the period when granulation tissue formation occurs are found, suggesting that a defect in V EGF regulation might be associated with wound healing disorders.

TL;DR: Rac and Cdc42 appear to regulate a protein kinase cascade initiated at the level of Pak and leading to activation of p38 and JNK, and a dominant negative Pak1 suppresses both interleukin-1- and Rac/Cdc42-induced p38 activity.

TL;DR: The isolation of a rat cDNA encoding a 150-kDa protein, which specifically binds RhoA in its GTP form and contains an N-terminal serine/threonine kinase domain highly related to the human myotonic dystrophy kinase and a cysteine-rich domain toward the C terminus is reported.

TL;DR: It is proposed that EEA1 is required for vesicular transport of proteins through early endosomes and that its finger motifs are required for this activity.

TL;DR: Results indicate that PPARs are differentially activated by naturally occurring eicosanoids and related molecules.