Showing papers in "Journal of Biological Chemistry in 2005"

TL;DR: It is suggested that low dose curcumin effectively disaggregates Aβ as well as prevents fibril and oligomer formation, supporting the rationale forCurcumin use in clinical trials preventing or treating AD.

TL;DR: It is found that sclerostin could antagonize canonical Wnt signaling in human embryonic kidney A293T cells and mouse osteoblastic MC3T3 cells and that s clergyostin bound to LRP5 as well as LRP6 and the first two YWTD-EGF repeat domains of L RP5 as being responsible for the binding.

TL;DR: It is shown that cells with targeted null mutations in Mfn1 or Mfn2 retained low levels of mitochondrial fusion and escaped major cellular dysfunction, suggesting a requirement for mitochondrial fusion, beyond maintenance of organelle morphology.

TL;DR: By specific knockdown of Oct4 and Sox2 mRNA by RNA interference in embryonic stem cells, this work provides genetic evidence for a link between Oct4, Sox2, and the Nanog promoter, extending the understanding of the pluripotent genetic regulatory network within which the Sox2-Oct4 complex are at the top of the regulatory hierarchy.

TL;DR: It is proposed that WNT/TCF1 signaling, like bone morphogenetic protein/transforming growth factor-β signaling, activates Runx2 gene expression in mesenchymal cells for the control of osteoblast differentiation and skeletal development.

TL;DR: It is demonstrated that RDC1 is expressed in T lymphocytes and that CXCL12-promoted chemotaxis is inhibited by an anti-RDC1 monoclonal antibody, and it is shown that CxCL12, the only known natural ligand for CXCR4, binds to and signals through R DC1.

TL;DR: It is proposed that binding of resveratrol to SIRT1 promotes a conformational change that better accommodates the attached coumarin group.

TL;DR: Observations that Aβ42 and other oligomers caused rapid cellular leakage of anionic fluorescent dyes point to a generalized increase in membrane permeability, which may provide a common mechanism for oligomer-mediated toxicity in many amyloidogenic diseases.

TL;DR: The cellular effects of overexpression of SIRT1, the closest mammalian ortholog of Sir2, are examined and it is demonstrated that SIRT 1 catalyzes PGC-1α deacetylation both in vitro and in vivo.

TL;DR: The data indicate that the CaMKKs function in intact cells as AMPKKs, predicting wider roles for these kinases in regulating AMPK activity in vivo.

TL;DR: The well ordered conformation of the helix-helix connector indicates a defined interaction with lipidic surfaces, suggesting that, when bound to larger diameter synaptic vesicles, it can act as a switch between this structure and a previously proposed uninterrupted helix.

TL;DR: The experiments demonstrate that the PTP can form and open, that CyP-D represents the target for PTP inhibition by CsA, and that Cyp-D modulates the sensitivity of the P TP to Ca2+ but not its regulation by the proton electrochemical gradient, adenine nucleotides, and oxidative stress.

TL;DR: Three novel TAG lipases/acylglycerol transacylases that likely participate in TAG hydrolysis and the acyl-CoA independent transacylation of acylglycerols, thereby facilitating energy mobilization and storage in adipocytes are identified.

TL;DR: It is shown here that SOST antagonizes Wnt signaling in Xenopus embryos and mammalian cells by binding to the extracellular domain of the Wnt coreceptors LRP5 and LRP6 and disrupting Wnt-induced Frizzled-LRP complex formation.

TL;DR: It is shown here that resveratrol is a substrate-specific activator of yeast Sir2 and human SirT1 and that in three different yeast strain backgrounds, resver atrol has no detectable effect on Sir2 activity in vivo, as measured by rDNA recombination, transcriptional silencing near telomeres, and life span.

TL;DR: It is demonstrated that in the Bcr-abl oncogene expressing human leukemia K562 cells,HDAC6 can be co-immunoprecipitated with HSP90, and the knock-down of HDAC6 by its siRNA induced the acetylation of HSP80 and α-tubulin, indicating that HDAC 6 is also an HSP 90 deacetylase.

TL;DR: A proteasome-dependent anti-amyloidogenic activity of resveratrol is demonstrated and it is suggested that this natural compound has a therapeutic potential in Alzheimer's disease.

TL;DR: This work shows that autophagy is induced in response to impaired ubiquitin proteasome system activity, and suggests that HDAC6-dependent retrograde transport on microtubules is used by cells to increase the efficiency and selectivity of autophagic degradation.

TL;DR: It is demonstrated that TLR3 contributes directly to the immune response of respiratory epithelial cells to influenza A virus and dsRNA, and a molecular mechanism by which these stimuli induce epithelial cell activation is proposed.

TL;DR: It is demonstrated that NF-κB signaling in microglia is critically involved in neuronal death induced by amyloid-β (Aβ) peptides, which are widely presumed to cause Alzheimer disease, and SIRT1 is implicate in this pathway.

TL;DR: It is reported here that murine SIRT3 is expressed in brown adipose tissue and is localized on the mitochondria inner membrane, and plays an important role in adaptive thermogenesis inbrown adipose.

TL;DR: It is reported that a single bout of voluntary running induced a transient increase of Pgc-1α mRNA expression in mouse plantaris muscle, concurrent with an activation of the p38 MAPK pathway, suggesting that contractile activity-induced activation ofThe p38MAPK pathway promotes PGC-1 α gene expression and skeletal muscle adaptation.

TL;DR: A gain-of-function mutation of JAK2 may explain the hypersensitivity of PV progenitor cells to growth factors and cytokines and is defined as a molecular defect of PV.

TL;DR: It is concluded that mTOR complexed to RICTOR is the Ser-473 kinase in 3T3-L1 adipocytes, and the apparently disparate results reported in these studies are difficult to evaluate, given that different stimuli and cell types were examined.

TL;DR: It is demonstrated that the entry of TAT peptide into different primary cells is ATP and temperature-dependent, indicating the involvement of endocytosis, and suggested that unconjugated peptide might follow endocytic pathways different from those utilized by T AT peptide conjugated to different proteins.

TL;DR: Direct evidence is provided for the involvement of ADAM17 in the regulated ectodomain shedding of ACE2 by using inhibitors with differing potency toward different members of the ADAM (a disintegrin and metalloproteinase) family of proteases.

TL;DR: It is shown that Klotho protein increases resistance to oxidative stress at the cellular and organismal level in mammals and potentially contributes to the anti-aging properties of klotho.

TL;DR: The results demonstrate that SphK1 and SphK2 have opposing roles in the regulation of ceramide biosynthesis and suggest that the location of sphingosine 1-phosphate production dictates its functions.

TL;DR: In this paper, the authors showed that the mechanism of photobiomodulation involves the up-regulation of cytochrome c oxidase, leading to increased energy metabolism in neurons functionally inactivated by toxins.

TL;DR: The mechanisms of the regulatory system FKBP51/FKBP52 discovered in yeast also operate in mammals to modulate hormone binding of the receptor and differential regulation of dynein association and nuclear translocation contributes to the effects of the two immunophilins on the glucocorticoid receptor in mammals.