Showing papers in "Journal of Biological Chemistry in 2016"

TL;DR: Transient transfections of different mutants demonstrated that Tyr-654 is phosphorylated in conditions in which adherens junctions are disrupted and evidenced that binding ofβ-catenin to E-cadherin in vivo is controlled by phosphorylation of β- catenin Tyr-652.

TL;DR: Studies with Gy mice and human patients with the very rare X-linked genetic condition Snyder-Robinson syndrome show clearly that the correct spermine:spermidine ratio is critical for normal growth and development.

TL;DR: The cyclic di-GMP second messenger represents a signaling system that regulates many bacterial behaviors and is of key importance for driving the lifestyle switch between motile loner cells and biofilm formers as mentioned in this paper.

TL;DR: Diverse biological processes that are dependent on PTM regulation of IDPs are discussed and recent tools for generating homogenously modified IDPs for studies of PTM-mediated IDP regulatory mechanisms are presented.

TL;DR: A genome-wide CRISPR/Cas9 screen in immortalized mouse macrophages aiming at the unbiased identification of gene products involved in Nlrp3 inflammasome activation is described, finding that targeting Nek7 rescued macrophage from nigericin-induced lethality.

TL;DR: The distinct metabolic signatures of key immune cells are described, how these metabolic setups facilitate immune function are explained, and the emerging evidence that intracellular metabolism has an integral role in controlling immune responses is discussed.

TL;DR: It is demonstrated that itaconate acts as an endogenous succinate dehydrogenase inhibitor to cause succinate accumulation and links the innate immune response and tricarboxylic acid metabolism to function of the electron transport chain.

TL;DR: This review presents key features of uORFs that serve to optimize translational control that is essential for regulation of cell fate in response to environmental stresses.

TL;DR: How calcium ended up in this privileged position is explored as its unique coordination chemistry was a decisive factor as it makes its binding by complex molecules particularly easy even in the presence of large excesses of other cations, e.g. magnesium.

TL;DR: This work describes a simple and economic electroporation-based strategy to deliver Cas9/sgRNA ribonucleoproteins into mouse zygotes with 100% efficiency for in vivo genome editing and uses CRISPR-EZ to target the tyrosinase (Tyr) gene.

TL;DR: Structural studies have demonstrated that βarrs have a conserved structure and activation mechanism, with plasticity of their structural fold, allowing them to adopt a wide array of conformations.

TL;DR: This work identified that teicoplanin, a glycopeptide antibiotic, potently prevents the entry of Ebola envelope pseudotyped viruses into the cytoplasm via high throughput screening of Food and Drug Administration-approved drugs.

TL;DR: The functional diversity of the CBP/p300 interactome provides an excellent example of the power of intrinsic disorder to facilitate the complexity of living systems.

TL;DR: It is shown here that insect and mammalian cells present similar microviscosity at their respective physiological temperature, indicating that both cell types combine regulation by sterols and phospholipids to control proper membrane fluidity.

TL;DR: Data show that hypoxia-inducible genes are regulated in a multilayered manner that includes epigenetic regulation via TETs and 5-hmC levels in addition to HIF stabilization.

TL;DR: The physiologic properties of primary cultures developed from isolated intestinal crypts or stem cells and from inducible pluripotent stem cells are discussed, their current use in understanding diarrhea-causing host-pathogen interactions, and potential future applications.

TL;DR: These studies provide the first evidence that fibronectin binding to heparan sulfate mediates exosome-cell interactions, revealing a fundamental mechanism important for exosomes-mediated cross-talk within tumor microenvironments.

TL;DR: It is clearly demonstrate that extracellular vesicles can transmit tau pathology, which indicates a role for extrace Cellular Vesicles in the transmission and spreading of t Tau pathology.

TL;DR: This review discusses the biosynthetic, evolutionary, and physiological processes that constrain or expand polyamine structural and functional diversity.

TL;DR: The clustering of distinct receptors in cell surface signaling platforms likely underlies the actions of distinct oligomeric species of Aβ and provides opportunities for therapeutic intervention in Alzheimer disease.

TL;DR: The solution is to use the root symbol SELENO followed by a letter, approved by the HUGO Gene Nomenclature Committee, which resolves conflicting, missing, and ambiguous designations for selenoprotein genes and is applicable toselenoproteins across vertebrates.

TL;DR: It is found that actin cytoskeletal integrity is essential for YAP nuclear localization, and can override phosphoregulation or contractility-mediated regulation of YAPnuclear localization independent of contractility.

TL;DR: Stable isotope-assisted metabolomics techniques are applied and it is demonstrated that pyruvate oxidation is maintained in mature pro-inflammatory macrophages and that the PDH flux is an important node for M(LPS) macrophage activation.

TL;DR: It is demonstrated that BCL2/adenovirus E1B 19-kDa interacting protein 3 (BNIP3), a mitochondrial BH3-only protein, interacts with PINK1 to promote the accumulation of full-length Pink1 on the outer membrane of mitochondria, which facilitates parkin recruitment and PINK 1/parkin-mediated mitophagy.

TL;DR: It is shown that HeLa epithelial cervical cancer cells and 143B osteosarcoma cells express a set of glutamine transporters including SNAT1, SNAT2,SNAT2 (SLC38A2), SNAT4, LAT1, and ASCT2, and a combined targeted approach would inhibit growth of glutamines-dependent cancer cells.

TL;DR: Deletion of aceA in P. aeruginosa improved cell growth under conditions of oxidative and antibiotic stress, consistent with the hypothesis that the glyoxylate shunt plays a previously unrecognized role in allowing bacteria to tolerate oxidative stress.

TL;DR: The current roles of exosomes in the pathology of neurodegenerative diseases are reviewed, showing that external stresses due to age-related impairment of protein quality control mechanisms can promote the transcellular flux of these proteins in exosome.

TL;DR: Results imply that exosomes containing TAR RNA could directly affect the proinflammatory cytokine gene expression and may explain a possible mechanism of inflammation observed in HIV-1-infected patients under cART.

TL;DR: First, alcohol increases EV production in monocytes; second, alcohol-exposed monocytes communicate with naive monocytes via EVs; and third, miR-27a cargo in monocyte-derived EVs can program naive monocyte to polarize into M2 macrophages.

TL;DR: It is demonstrated that mitochondrial dysfunction also disrupts the structure and function of lysosomes, the main degradation and recycling organelle and provides important insights into the etiology of neurodegenerative diseases.