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Showing papers in "Journal of Bioscience and Bioengineering in 2001"


Journal ArticleDOI
TL;DR: Biodiesel (fatty acid methyl esters), which is derived from triglycerides by transesterification with methanol, has attracted considerable attention during the past decade as a renewable, biodegradable, and nontoxic fuel.

2,185 citations


Journal ArticleDOI
TL;DR: In this article, the authors stress the importance and interesting aspect of protein adsorption on solid surfaces by reviewing findings that have been obtained in recent years, and stress that information on the conformation of the adsorbed protein as well as adsorbing characteristics is essential for a system's performance.

890 citations


Journal ArticleDOI
TL;DR: In this article, several physical and chemical methods, such as immobilization, modification, and entrapment, for stabilizing enzymes in the presence of organic solvents were developed.

299 citations


Journal ArticleDOI
TL;DR: A high yield of hydrogen of 7.0 mol H2/mol glucose from the starch remaining in the starch residue was attained in two-step repeated batch cultures containing C. butyricum and E. aerogenes, and by Rhodobacter sp.

298 citations


Journal ArticleDOI
TL;DR: In this article, the methanolysis of soybean oil by lipases from various microorganisms was investigated, and several of the lipases were found to catalyze methanization in a water-containing system without an organic solvent.

256 citations


Journal ArticleDOI
TL;DR: In Hypocrea jecorina and Penicillium purpurogenum, the respective inducers are sophorose and gentiobiose, which do not have beta-1,4 linkages though cellobiose , which has this linkage, is an inducer in other fungi.

248 citations


Journal ArticleDOI
TL;DR: The Liposome consisting of eggPC, cholesterol, and DSPE-PEG5000 with a molar ratio of 1.5:1:0.08 was used to entrap cell-free protein synthesis reaction mixture and the synthesis of a mutant green fluorescent protein in the liposome was confirmed by the fluorescence emitted from theliposome on flow cytometry analysis and fluorescence microscopy.

222 citations


Journal ArticleDOI
TL;DR: The findings that recently advanced the understanding of various aspects of PTS-dependent carbohydrate transport and regulation of bacterial catabolism are reviewed.

197 citations


Journal ArticleDOI
TL;DR: The results show that either one of alanine, sorbitol, mannitol or trehalose can be used as a sole carbon and energy source for P. pastoris, although the doubling time on tre Halose was very long.

183 citations


Journal ArticleDOI
TL;DR: Fluorescence in situ hybridization (FISH), in situ PCR, and quantitative PCR are expected to be powerful tools for bioremediation to detect and enumerate the target bacteria that are directly related to the degradation of contaminants.

156 citations


Journal ArticleDOI
TL;DR: A pH control strategy for cell growth and epsilon-PL production is suggested aimed at increasing the amount of epsilon-PL produced.

Journal ArticleDOI
TL;DR: The results suggest that Ni2+ and Co2+ were required for the methane-producing reactions via increases of coenzymes F430 and corrinoids.

Journal ArticleDOI
TL;DR: B. subtilis WU-S2B is considered to have more beneficial properties than other desulfurizing bacteria such as Rhodococcus strains previously reported, particularly from the viewpoint of its capacity for thermophilic desulforization through the CS bond cleavage.

Journal ArticleDOI
TL;DR: It was found that, compared with an agitated and aerated culture, production of MK-7 was increased by static culture, and the sporulation of the cells progressed more slowly in a static culture than in an agitated culture.

Journal ArticleDOI
TL;DR: In this paper, co-immobilized cells of Saccharomyces diastaticus and Zymomonas mobilis produced a high ethanol concentration compared to immobilized cells during batch fermentation of liquefied cassava starch.

Journal ArticleDOI
TL;DR: The diversity of microbial community during the decomposition of waste in a field-scale composter (Hazaka system) was investigated by denaturing gradient gel electrophoresis (DGGE) and 16S rDNA analyses showed that the following genera were found throughout the process.

Journal ArticleDOI
TL;DR: In this paper, the authors used formol titration for measuring assimilable nitrogen in the must to distinguish two kinds of fermentation problems: slow and late-onset sluggish fermentations and stuck fermentations.

Journal ArticleDOI
TL;DR: The chitinolytic bacterium Clostridium paraputrificum strain M-21 produced 2.2 and 1.5 mol hydrogen gas from 1 mol Nacetyl-d-glucosamine (GlcNAc) and ball-milled chitin equivalent to 1 mol of Glc NAc, respectively, at pH 6.0 as discussed by the authors.

Journal ArticleDOI
TL;DR: In this paper, a new method of protein redox potential measurements and a novel electrochemical kinetic analysis of oxidoreductase reactions are described, and a new methodology of characterizing microbial catalytic activities is presented.

Journal ArticleDOI
TL;DR: A yeast strain producing high levels of phytase was isolated from soil and identified as Candida krusei and was a glucanase-extractable protein, which showed two optimum pHs and an optimum temperature of 40 degrees C.

Journal ArticleDOI
TL;DR: No generalising pattern with respect to influence of metal speciation on degradation can be deduced yet and information on the transport of APCAs is presently limited to EDTA, where there is strong evidence for an energy-dependent carrier that can transport free and Ca2+-complexed EDTA.

Journal ArticleDOI
TL;DR: In this article, a strain of Acinetobacteriobacter sp. was identified and characterized for the ability of the strain to assimilate long-chain n-paraffins.

Journal ArticleDOI
TL;DR: It is suggested that during the LHW pretreatment, alfalfa fiber nutrients are lost in cellulosic fractions but retained in hemicellulosic extract fractions.

Journal ArticleDOI
TL;DR: Results strongly suggest that the two strains isolated from deep subterranean petroleum reservoirs degraded n-alkanes by a terminal oxidation pathway, followed by a beta-oxidation pathway.

Journal ArticleDOI
TL;DR: The yield and production rate of hydrogen by R. marinum A-501 from the lactic acid fermentates were higher than from authentic lactic Acid, suggesting that the fermentates contain a factor(s) which promotes H2 production by this bacterium.

Journal ArticleDOI
TL;DR: The hydrogen production yield from glucose by an isolate was investigated and compared to that by microflora, implying that T. thermosaccharolyticum could be a predominant species of the micro flora that is involved in hydrogen-producing acetate/butyrate fermentation.

Journal ArticleDOI
TL;DR: Strain no. 6 extracellularly produced a lipolytic enzyme that efficiently hydrolyzed triglycerides such as soybean oil during bacterial growth even at 4 degrees C, which is potentially applicable to in-situ bioremediation or bioaugumentation of fat-contaminated cold environments.

Journal ArticleDOI
TL;DR: Four inorganic packing materials were evaluated in terms of their availability as packing materials of a packed tower deodorization apparatus (biofilter) from the viewpoints of biological H2S removal characteristics and some physical properties.

Journal ArticleDOI
TL;DR: The PAP-PPK ATP regeneration system can regenerate GTP from GMP, it could also be used as a GTP regeneration system and succeeded in synthesizing acetyl-CoA from CoA, acetate and AMP.

Journal ArticleDOI
TL;DR: Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction and purified total DNA, total RNA and plasmids at an efficiency comparable to that of manual methods.