Showing papers in "Journal of Biotechnology in 2005"

TL;DR: Issues addressed include expression systems in general, selection of host strain, mRNA stability, codon bias, inclusion body formation and prevention, fusion protein technology and site-specific proteolysis, compartment directed secretion and finally co-overexpression technology.

TL;DR: The SF nanofiber membrane was shown to possess good biocompatibility with enhanced bone regeneration and no evidence of any inflammatory reaction, suggesting that the SF membrane should be useful as a tool for guided bone regeneration.

TL;DR: Modification of the enzymes immobilized on supports that did not offer the possibility of react with glutaraldehyde showed the same stability that the non modified preparations demonstrating that the mere chemical modification did not have effect on the enzyme stability.

TL;DR: It is demonstrated that gene expression events monitored in the liver can be used to predict pathological events occurring in that tissue as well as in hematopoietic tissues.

TL;DR: Cultivation of Dunaliella in closed tubular photobioreactor represents a suitable approach for the production of a high-quality microalgal biomass enriched in the valuable 9-cis-isomer of beta-carotene and lutein.

TL;DR: This study unambiguously confirms hydrolysis using cutinase of the persistent synthetic polymer poly(ethylene terephthalate), the most important synthetic fiber in the textile industry by direct measurement and identification of the different hydrolytic products.

TL;DR: A mixture of linear and branched short chain alcohols that mimics the residual of ethanol distillation (fusel oil) was successfully used for oleic acid ester synthesis, which are important in biodiesel mixtures since they improve low temperature properties.

TL;DR: A modified Gompertz equation adequately described fermentative hydrogen production from cheese whey and found that lag phase times were much longer at acidic pHs than at alkaline pHs.

TL;DR: The results indicate that the two-phase hybrid approach presents the best compromise between robustness and efficiency.

TL;DR: P pH decreased during bioleaching, but remained relatively constant in both leaching using fresh medium and cell-free spent medium, thus indicating that the fungus played a role in effecting metal extraction from the spent catalyst.

TL;DR: A tidal flow constructed wetland system was investigated for the removal of organic matter and ammoniacal-nitrogen from diluted piggery wastewater and it was demonstrated that the operation of tidal flow enhanced the transfer of oxygen into wetland matrices.

TL;DR: Investigation of the ability of double-stranded RNA (dsRNA) to inhibit myostatin function in the zebrafish produced an increased body mass in treated fish and the interference of gene function showed a strong dependence on the amount of dsRNA.

TL;DR: Both an upper limit on agitation intensity and a high level of dissolved oxygen are essential for attaining high titers of lovastatin in fungal pellet morphology and broth rheology.

TL;DR: The metabolic pathway of the strain relating to starch and pyruvate degradation is discussed, and the level of H2 appeared to correlate with the specific growth rate, analysis in continuous cultures was performed to develop a continuous H2 production system.

TL;DR: Determination of the number of surface amine groups and optimization of DNA capture condition were demonstrated and performance of PCR for the eluted fraction indicates that DNA extracted from whole blood was well purified using amine-coated microchip.

TL;DR: Overall, this study shows the possible use of bioleaching for the extraction of metal resources from spent catalysts and demonstrated the advantages of buffer-stimulated excretion of organic acids by A. niger inBioleaching of the spent catalyst.

TL;DR: The feasibility of using recombinant laccase for bleaching high-quality non-wood pulps in the presence of a mediator is revealed, resulting in high-final brightness values that are difficult to attain by chemical bleaching of this type of pulp.

TL;DR: It is indicated that elicitor treatment promotes secondary metabolite production in S. marianum cultures and that jasmonic acid and its functional analogue plays a critical role in elicitation.

TL;DR: Acidocin D20079 has a narrow inhibitory spectrum restricted to the genus Lactobacillus which includes L. sakei NCDO 2714, an organism known to cause anaerobic spoilage of vacuum-packaged meat.

TL;DR: The white rot basidiomycete, Phanerochaete chrysosporium, employs an array of extracellular enzymes to completely degrade the major polymers of wood: cellulose, hemicellulose and lignin.

TL;DR: Beta-glucosidase genes of fungal origins were isolated and heterologously expressed in Saccharomyces cerevisiae to enable growth on the disaccharide, cellobiose, and the resulting recombinant enzymes were characterized with respect to pH and temperature optimum, as well as kinetic properties.

TL;DR: A continuous acetone-butanol-ethanol (ABE) production system with high cell density obtained by cell-recycling of Clostridium saccharoperbutylacetonicum N1-4 has been studied and cell-bleeding was performed together with cell- recycling to maintain a stable cell concentration.

TL;DR: The introduction of an acyl group into glucosylated flavonoids significantly improved their antioxidant activity towards both LDL and serum model in vitro and increased its antimicrobial activity against two Gram-positive bacteria.

TL;DR: An increase in ROL gene dosage seems to negatively affect cell's performance in bioreactor cultures, particularly in Mut(s) strains, which may be still advantageous to use because it allows for easier process control strategies.

TL;DR: The results suggested that the MTGase treatment of film-forming solutions of SPI prior to casting could greatly modify the properties and microstructures of SPI films.

TL;DR: An efficient one-step chromatography method for purification of R-phycoerythrins from Polysiphonia urceolata was described in this article, which was obtained with an absorbance ratio A(565)/A(280) of 5.6 and a high recovery yield of 67-33%.

TL;DR: Thiol-linked DNA-gold nanoparticles were used in a novel colorimetric method to detect the presence of specific mRNA from a total RNA extract of yeast cells, allowing detection of expression of the FSY1 gene that encodes a specific fructose/H+ symporter in Saccharomyces bayanus PYCC 4565.

TL;DR: The recombinant T2 phage, named T2ppD1, carried PP01 gp37 and 38 and infected the heterogeneous host cell E. coli K12, the original host of T2, or its derivatives, but could not infect E. Escherichia coli O157:H7 and related species.

TL;DR: The surface carboxylic groups of penicillin G acylase and glutaryl acyl enzyme were chemically aminated in a controlled way by reaction with ethylenediamine via the 1-ethyl-3-(dimethylamino-propyl) carbodiimide coupling method and were immobilized on glyoxyl agarose.

TL;DR: The potential for using tyrosinase to graft the polysaccharide chitosan (Ch) onto Bombyx mori silk fibroin (SF) was examined and FT-IR spectroscopy revealed that grafting induced a beta-sheet --> random coil conformational transition.