Showing papers in "Journal of Biotechnology in 2011"

TL;DR: Lignocellulosic biomass is a promising feedstock for lactic acid production considering its great availability, sustainability, and low cost compared to refined sugars.

TL;DR: It is shown that despite rapid progress in the experimental and genomic analyses of pure cultures, knowledge on the mechanism of nitrate reduction in natural ecosystems is still largely lacking.

TL;DR: It was found that the use of a continuous feeding strategy rather than a pulse feeding strategy can not only help mitigate the process constraints of the pulse-feeding strategy (resulting in higher specific and volumetric productivities) but also be used as means to broaden the range of polymer structures.

TL;DR: It is demonstrated that blood samples can be used as a source of miRNA identification associated to Parkinson's disease (PD) and a set of six differentially expressed microRNAs were identified.

TL;DR: Heterologous expression of the araBAD operon from Escherichia coli in the wild-type and in an l-lysine producing strain of C. glutamicum was shown to enable production of l-glutamate and l- Elysine, respectively, from arabinose as sole carbon source.

TL;DR: The complete DNA sequence of the first mitochondrial genome of a methylotrophic yeast is reported, and the architecture of the putative alpha mating factor protein of P. pastoris CBS7435 turned out to be more complex than the corresponding protein of Saccharomyces cerevisiae.

TL;DR: Downregulating LDHa and PDHKs in CHO cells expressing a therapeutic monoclonal antibody reduced lactate production, increased specific productivity and volumetric antibody production by approximately 90%, 75% and 68%, respectively, without appreciable impact on cell growth.

TL;DR: A spontaneous mutant, FB01mut, emitting bright fluorescence was detected among the transformants and found suitable for colonization experiments performed with Zea mays, Arabidopsis thaliana and Lemna minor, and is an excellent tool for studying plant colonization under competitive, environmental conditions.

TL;DR: This work presents the first comprehensive comparison of V gene subfamily use for all steps of an antibody phage display pipeline, and describes a compatible cassette vector set, allowing in vivo biotinylation, enzyme fusion and Fc fusion.

TL;DR: The findings suggest that genetically engineered LAB that produce antioxidant enzymes could be used to prevent or decrease the severity of certain intestinal pathologies.

TL;DR: It is demonstrated that dissociation constants in the extremely low micromolar range, which were lower than those previously reported for Cam-specific RNA aptamers, were achieved and open up possibilities to allow easy detection of Cam via aptamer-based biosensors.

TL;DR: The generation of a cisgenic apple plant is reported by inserting the endogenous apple scab resistance gene HcrVf2 under the control of its own regulatory sequences into the scab susceptible apple cultivar Gala.

TL;DR: Differences established within the variable part of the genomes, justify the proposal to discriminate the plant-associated ecotype represented by FZB42 from the group of type strain related B. amyloliquefaciens soil bacteria.

TL;DR: It seems that σ(H) occupies a central position in the cross-regulated network of sigma factors and controls their concerted response to various stress conditions in C. glutamicum.

TL;DR: In this study, a thin-layer (10mm) flat plate photobioreactor device with a light/dark (L/D) alternation system is constructed to investigate the growth of Spirulina platensis under two different light regimes.

TL;DR: The present manuscript represents a data set of assembled and annotated genes for CHO cells that can now be used for a detailed analysis of the molecular functioning of CHO cell lines.

TL;DR: It is demonstrated that cell propagation and lipid accumulation processes can be spatially separated, allowing further optimization to improve both processes to develop more efficient processes to convert renewable materials into biofuel and related products.

TL;DR: The results demonstrated that engineered derivatives of strain BF13 accumulate vanillin if inactivation of vdh is associated with concurrent expression of structural genes for feruloyl-CoA synthetase (fcs) and hydratase/aldolase (ech) from a low-copy plasmid.

TL;DR: Deep-sequencing small RNA fractions of 6 biotechnologically relevant cell lines and cDNA sequencing of 26 validated targets of miR-17-92 suggests conserved functions for miRNAs in CHO cells, which together with the now publicly available sequence information sets the stage for developing novel RNAi tools for CHO cell engineering.

TL;DR: The amounts of both PEI and plasmid DNA were reduced up to 50% on a per cell basis compared to previously published protocols, resulting in higher cell viability after transfection and higher volumetric recombinant protein yields.

TL;DR: The complete genome sequence of Agrobacterium sp.

TL;DR: Flexible residues at high temperature in unstable regions can be promising targets to improve thermostability of enzymes and are confirmed by computational modeling instead of random mutagenesis.

TL;DR: This is the first report demonstrating a functional impact of specific miRNA disregulation on CHO cell behavior in batch culture and provides some evidence of the potential which these molecules may have in terms of engineering targets in CHO production clones.

TL;DR: The present study investigates the effect of pH and intermediate products formation on biological hydrogen production using Enterobacter cloacae IIT-BT 08, found to have a profound effect on hydrogen production potential, while regulating the pH 6.5 throughout the fermentation was found to increase the cumulative hydrogen production rate and yield significantly.

TL;DR: The ability to control the disulfide bond formation in VLPs is demonstrated by directly controlling the redox potential during or after production and assembly of VLPS.

TL;DR: It is shown that the functionalization of liposomes containing acidic phospholipids with the ApoE fragment 141-150 scarcely affects their reported ability to bind Aβ peptide in vitro, and are important and promising features for the possibility to use these nanoliposomes for the targeting of Aβ in the brain districts.

TL;DR: The effect of operation regime and culture system on carotenoid productivity by the halotolerant alga Dunaliella salina has been analyzed and closed tubular photobioreactor provided the best results.

TL;DR: This work demonstrates that genetically engineered bacteria can be used to synthesize QDs, and these biologically synthesized QDs are expected to be more biocompatible probes in bio-labeling and imaging.

TL;DR: FAO and other relevant international organisations and donors should significantly increase their efforts to support the strengthening of national capacities in the development and appropriate use of pro-poor agricultural biotechnologies.

TL;DR: In this paper, the authors reported further metabolic engineering of E. coli strain to overcome these problems for more efficient production of PLA and its copolymers without adding inducer and succinate, and the finally constructed recombinant E coli JLXF5 strain was able to produce P(3HB-co-39.6 mol% LA) having the molecular weight of 141,000 Da to 20 g l»¹ with a polymer content of 43 wt% in a chemically defined medium by the pH-stat fed-batch culture.