Journal•ISSN: 0095-9898
Journal of Cellular and Comparative Physiology
Wiley
About: Journal of Cellular and Comparative Physiology is an academic journal. The journal publishes majorly in the area(s): Potassium & Yeast. It has an ISSN identifier of 0095-9898. Over the lifetime, 2272 publications have been published receiving 73366 citations.
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1,566 citations
TL;DR: The spleen colony method is a quantitative method for the detection of progenitor cells that are capable of self-renewal and extensive proliferation in the spleens of irradiated mice as mentioned in this paper.
Abstract: Progenitor cells that are recognized by their ability to form colonies of descendants in the spleens of irradiated mice have the capacity for self-renewal. The distribution of new colony-forming cells per colony is extremely heterogeneous, indicating lax control of self-renewal. The capacities of colony-forming cells for self-renewal, for extensive proliferation, and for giving rise to differentiated descendants, fulfill three requirements for studies of stem cells. Thus, colony-forming cells may be considered to be class (though not necessarily the only class) of such progenitor cells, and the spleen colony method is a quantitative method for their detection. Journal of Cellular and Comparative Physiology copyright 1963 Wiley-Liss, Inc., A Wiley Company (www.interscience.Wiley.com).
922 citations
871 citations
TL;DR: The results indicate that the substance(s) required for the growth and differentiation of “mast” cells can pass through agar.
Abstract: A method is described for the cloning of normal mouse “mast” cells in tissue culture in a soft agar medium. The colonies contain cells in different stages of differentiation. It was shown that a colony can be initiated by a single colony forming unit, and that colonies are formed as a result of cell multiplication.
Cell suspensions from adult spleen gave about 3 colonies per 105 cells seeded. A re-cloning of these colonies gave about 3 colonies per 103 cells seeded.
The frequency of colonies from SWR mice was higher with adult spleen than with adult thymus. No such colonies were obtained with adult lymph node cells.
The formation of colonies was shown to require the presence of an embryo cell feeder layer. Since the feeder layers were seeded underneath the agar, the results indicate that the substance(s) required for the growth and differentiation of “mast” cells can pass through agar.
832 citations