Showing papers in "Journal of Chromatography B: Biomedical Sciences and Applications in 2002"

TL;DR: In this article, the relevance of weighting schemes for linear regression analysis and how this approach can be useful in the bioanalytical field is discussed and the steps to be taken in the study of the linear calibration approach are described.

TL;DR: Ion suppression effects during electrospray-ionsation mass spectrometry (ESI-MS) caused by different sample preparation procedures for serum were investigated in this article, with continuous postcolumn infusion of two test compounds-codeine and glafenine-the ion suppression effects of extracted biological matrix obtained after a standard liquid-liquid extraction, a mixedmode solid-phase extraction method, a protein precipitation method and a combination of precipitation with polymer-based mixed-mode SPE have been investigated.

TL;DR: The role of 1-hydroxypyrene or phenanthrene metabolites as established biomarkers and also more recent developments of PAH biomonitoring are discussed in this article.

TL;DR: The efficiency of acetonitrile extraction was demonstrated in mass balance evaluations of toasting of soy flour and soymilk heating.

TL;DR: Since GSH, GSSG, and related compounds lack of strong chromophores or fluorophores, it is advantageous, in many assays, to derivatize the compounds in order to improve the detection limit with UV-Vis and to allow fluorescence, thus the most commonly used labeling agents are described.

TL;DR: In this paper, a review of recent trends in the estimation of binding constants by affinity electrophoresis is presented, in which the stoichiometry of the binding between drug and protein is assumed to be 1:1 as a simple model.

TL;DR: In this article, a review of the use of high-performance affinity chromatography (HPAC) in biological systems is presented, focusing on the techniques of zonal elution and frontal analysis.

TL;DR: In this paper, a Tomtec Quadra 96 (Model 320) and a protein precipitation step in a 96-well plate format were used for detection of signal transduction inhibitor STI571 and its main metabolite (CGP 74588) in human plasma.

TL;DR: Deeper knowledge has to be reached in order to evaluate the significance of elements and, possibly, of their species in biological fluids at current doses and to face their effects, especially those in the first portion of the dose-response curve, which is going to be the main field of interest of occupational and environmental toxicology for the next few years.

TL;DR: In this paper, a method for simultaneous quantitative determination of various aromatic amines (or metabolites of aromatic nitro compounds, respectively) in human urine in one analytical run was proposed.

TL;DR: This paper reviews the evidence in humans and animals for anti-thyroid effects of soy and its principal isoflavones, genistein and daidzein.

TL;DR: In this paper, the inner capillary wall was coated with polyvinyl alcohol in order to obtain baseline resolution of catecholamines and metanephrines and to ensure reproducibility without extensive restorative washing of the capillary.

TL;DR: The major urinary metabolite of selenium (Se) in rats was identified by HPLC-inductively coupled argon plasma mass spectrometry (ICP-MS) and ESI-MS/MS and assigned as a diastereomer of a selenosugar, Se-methyl-N-acetyl-selenohexosamine.

TL;DR: In this paper, a method was proposed for the determination of nicotine and cotinine in human urine, plasma and saliva, and the results showed that through the accurate determination of cotinines in saliva, the risk of ETS-exposed human can be predicted.

TL;DR: In this article, a new determination procedure for selective serotonin reuptake inhibitors (SSRIs), serotonin noradrenergic re-uptake inhibitor (SNaRIs) and specific serotoninergic antidepressant (NaSSA) was developed by micellar electrokinetic capillary chromatography (MEKC) with diode array detection.

TL;DR: Capillary isotachophoresis-capillary zone electrophoresis (ITP-CZE) is an easy way to increase the sample loadability and sensitivity and is therefore an alternative tool to the existing HPLC methods for analyzing the residuals of these quinolones in biological samples.

TL;DR: A review of existing analytical methods for plant estrogens (phytoestrogens) has been presented in this article, highlighting the advantages and disadvantages of each of these methods and potential areas for further development identified.

TL;DR: The characteristics, performances and limits of the main current homocysteine analytical methods are reviewed and one major drawback among all these available methods is the transferability which is not clearly established to date.

TL;DR: A review of 2D liquid separation methods used in our laboratory to map the protein content of human cancer cells is presented in this paper, where various means of fractionating proteins according to isoelectric point (pI) in the first dimension are discussed.

TL;DR: An RP-HPLC method for the determination of daidzein, genistein, formononetin and biochanin A in red clover (Trifolium pratense L.) was developed and validated as mentioned in this paper.

TL;DR: In this article, a rat urine sample was measured after a single p.o. dose of an equivalent quantity of secoisolariciresinol diglycoside (SDG), SecoSrinivasan et al. showed that the aglycone form of SECO was more efficiently converted into mammalian lignans endolactone (ENL) and ENL than the glycosylated form, SDG.

TL;DR: In this article, a method based on liquid chromatography tandem mass spectrometry (LC-MSMS) applying atmospheric pressure chemical ionisation (APCI) in the positive ion mode was developed for the direct determination of nicotine, cotinine, trans-3'-hydroxycotinine, their corresponding glucuronide conjugates as well as cotinines-N-oxide, norcotinines, and nicotine-N'-oxide in the urine of smokers.

TL;DR: A liquid chromatography-tandem mass spectrometry method was developed for the analysis of sildenafil and its metabolite desmethylsildanafil in human plasma and validated to meet the criteria of current industrial guidance for quantitative bioanalytical methods.

TL;DR: In this paper, a detailed protein composition of CSF by two-dimensional gel electrophoresis and to identify both high and low concentrated proteins using different mass spectrometry techniques.

TL;DR: In this paper, a photodiode array UV-Vis detector detected nitrite and nitrate ions at an absorbance of 212 nm and 3.72 min by electrochemical detection and 4.44 min by HPLC, respectively.

TL;DR: Olanzapine was shown to be stable in plasma for up to 7 days when stored at 4 degrees C and the addition of ascorbic acid was not necessary for the achievement of chemical stability during storage, or during the assay procedure.

TL;DR: In this paper, the use of a high-performance liquid chromatographic procedure with electrochemical detection for the measurement of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) was described.

TL;DR: In this paper, the uptake of creatine as a result of creatine supplementation, a practice increasingly common among bodybuilders and athletes, has lead to a need to measure urinary creatine concentrations.

TL;DR: In this article, the authors used carbon-13 labeled internal standards, enzymatic hydrolysis, and solid phase microextraction followed by gas chromatography with high-resolution mass spectrometry to detect polycyclic aromatic hydrocarbons (PAHs) in human urine.

TL;DR: In this article, the authors proposed a glycome project specifically focusing on glycoproteins, which identifies which genes encode glycoprotein (i.e. genome information); which sites among potential glycosylation sites are actually glyco-lated (i.,e. structural information); and what are the effects (functions) of glycosyation (functional information).