Journal of Contemporary Urologic and Reproductive Oncology
About: Journal of Contemporary Urologic and Reproductive Oncology is an academic journal. The journal publishes majorly in the area(s): Gene expression & Cystectomy. Over the lifetime, 15 publication(s) have been published receiving 28 citation(s).
TL;DR: It appears that TRPM8 was not essential for the survival of PC-3 cells; however, the overexpression of TR PM8 had negative effects on the proliferation and migration of PC -3 cells.
Abstract: Objective To investigate the effects of transient receptor potential M8 (TRPM8) channel on the proliferation and motility of androgen-independent prostate cancer PC-3 cells.Methods After being permanently transfected with an empty vector and cDNA encoding the TRPM8 protein,cells were analysed for cell cycle distribution and motility using flow cytometry and scratch assay.Results Immunocytochemistry and Ca2+ imaging analysis revealed the overexpression of functional TRPM8 channel on both endoplasmic reticulum and plasma membrane of PC-3-TRPM8 cells.Cell cycle distribution and scratch assay analysis revealed that TRPM8 induced cell cycle arrest at the G0/G1 stage ( 71.89% 3.43% vs 54.67%±4.59%、51.48%±3.54%,P0.05 ) and facilitated the cell apoptosis induced by starvation ( 12.56%±1.78% vs 4.67%±1.49%、5.28±1.35%,P0.05 ).Furthermore,TRPM8 inhibited the migration of PC-3-TRPM8 cells (P0.01) through the inactivation of focal-adhesion kinase.Conclusions It appears that TRPM8 was not essential for the survival of PC-3 cells;however,the overexpression of TRPM8 had negative effects on the proliferation and migration of PC-3 cells.Thus,TRPM8 and its agonists may serve as important targets for the treatment of prostate cancer.
TL;DR: This new non-invasive diagnostic application of the FISH technique with high detection rate in the field of urology is prior to urinary cytology.
Abstract: Objective To evaluate the clinical significance of fluorescence in situ hybridization(FISH)technique for screening bladder transitional cell carcinoma with hematuria,provide a new method for early diagnosis of bladder cancer with cytology of the urine.Methods We selected 20 relatively healthy people as control group.The special urine examination method detects genetic alterations of the urothelial cells found in the urine of the control group,using fluorescent direct labeled DNA probes binding to the peri-centromeric regions of chromosomes 3,7 and 17 as well as on the 9p21 locus,respectively.Thresholds were then established.Morning urine samples collecting from 100 patients with hematuria underwent cytology of the urine and FISH examination,respectively.The sensitivity and specificity of both were analyzed statistically.Results The complete absent,the partial absent and amplification thresholds for p16 gene detected by probes were 3.9%,5.4% and 3.2%,respectively.The absent and amplification thresholds for chromosome 3 were 4.0% and 3.5%;4.0 % and 2.8% for chromosome 7;5.8% and 3.4% for chromosome 17 respectively.The study rated the specificity and sensitivity of the technique 93.6% and 100%,respectively.The study rated the specificity and sensitivity of the urinary exfoliative cytology 46.8% and 100%,respectively.Conclusions This new non-invasive diagnostic application of the FISH technique with high detection rate in the field of urology is prior to urinary cytology.
TL;DR: Bilateral renal with cystic and solid mass the disease should be considered and focal excision with nephron sparing surgery is the primary therapy and selection pregnancy with gene detection is critical to prevent it occurrence and heredity.
Abstract: Objective To improve the diagnosis and treatment of von Hippel-Lindau syndrome.Methods Retrograde analyze the diagnosis and treatment of 8 cases of von Hippel-Lindau syndrome,4 received the operations of bilateral focal excision and renal cyst decortications,2 received unilateral focal excision and 2 only received regular follow-up without any operation.Results There were 4 individuals recovery,3 patients survival with tumor and 1 case death.Conclusions Bilateral renal with cystic and solid mass the disease should be considered,focal excision with nephron sparing surgery is the primary therapy and selection pregnancy with gene detection is critical to prevent it occurrence and heredity.
TL;DR: The dsRNAp21-pGenesil-1 express ds RNA molecule successfully, active the level of expression of p21 mRNA and protein, and provide an effective tool for studying the activation effect of dsRNAC on the basis of human prostate cancer cell line PC-3 in vitro.
Abstract: Objective To construct the small-molecule double-stranded RNA (dsRNA) expression vector plasmid,and to investigate its activation ability of the expression of cell cycle repressor protein p21WAF1/CIP1(p21) in human prostate cancer cell line PC-3 in vitro.Methods Synthetic the sequence-specific corresponding DNA fragments of the promoter with the p21 gene,as well as the same length of random DNA fragments,by means of the recombinant DNA technology to construct these fragments into the eukaryotic expression plasmids pGenesil-1,constructed with the purpose of the expression of dsRNA fragments plasmid vectors (dsRNAp21-pGenesil-1) and the random fragments of the control dsRNA expression vectors (dsRNACon-pGenesil-1).The dsRNAp21-pGenesil-1 (experimental group),dsRNACon-pGenesil-1 (negative control group) and a blank plasmid pGenesil-1 (control group) were transfected into human prostate cancer cell line PC-3 in vitro respectively with lipofectamine reagent,and transfection efficency was detected by laser scanning confocal microscope.Immunohistochemistry and reverse transcription-PCR were performed to examine the difference expression level of p21 in each group.Results These dsRNAp21-pGenesil-1 and the dsRNACon-pGenesil-1 eukaryotic cell expression vectors were successfully constructed.For each of the groups of plasmids transfected into PC-3 cells,p21 gene expression in the experimental group significantly increased,while there was no significant difference between the negative control group and the blank control group.Conclusions The dsRNAp21-pGenesil-1 express dsRNA molecule successfully,and active the level of expression of p21 mRNA and protein,and provide an effective tool for studying the activation effect of dsRNA.
TL;DR: The case was proved to be mature adrenal teratoma by pathology, and within one year follow-up, no tumor recur or metastasis was observed.
Abstract: Objective To study the clinical feature of teratoma in adrenal gland Methods One case of adrenal teratoma treated in our institution were reported,and the clinical manifestations,diagnosis and treatment were analyzed retrospectively under review of literature Results A 45-year-old woman with a 3-days history of the right waist and back pain received ultrasound and computed tomographic scan of the abdomenThe examination of Ultrasound revealed adrenal malignant tumor;and CT scans indicated teratoma or hamartomaThe tumor was resected by surgery,and the case was proved to be mature adrenal teratoma by pathologyWithin one year follow-up,no tumor recur or metastasis was observed Conclusions The adrenal teratoma is a rare germ cell tumorClinical symptoms and signs are not typicalImaging examination is important for diagnosisThe teratoma could be diagnosed by imaging examinationPathological examination is the fundamental basis for the diagnosis of adrenal teratomaEarly diagnosis and surgical treatment is recommendedThe prognosis of the mature teratoma is good after the operationThe relapse of immature teratoma is commonChemotherapy and lifelong follow-up is recommended
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