Showing papers in "Journal of Contemporary Urologic and Reproductive Oncology in 2009"
Journal Article•
TL;DR: It appears that TRPM8 was not essential for the survival of PC-3 cells; however, the overexpression of TR PM8 had negative effects on the proliferation and migration of PC -3 cells.
Abstract: Objective To investigate the effects of transient receptor potential M8 (TRPM8) channel on the proliferation and motility of androgen-independent prostate cancer PC-3 cells.Methods After being permanently transfected with an empty vector and cDNA encoding the TRPM8 protein,cells were analysed for cell cycle distribution and motility using flow cytometry and scratch assay.Results Immunocytochemistry and Ca2+ imaging analysis revealed the overexpression of functional TRPM8 channel on both endoplasmic reticulum and plasma membrane of PC-3-TRPM8 cells.Cell cycle distribution and scratch assay analysis revealed that TRPM8 induced cell cycle arrest at the G0/G1 stage ( 71.89% 3.43% vs 54.67%±4.59%、51.48%±3.54%,P0.05 ) and facilitated the cell apoptosis induced by starvation ( 12.56%±1.78% vs 4.67%±1.49%、5.28±1.35%,P0.05 ).Furthermore,TRPM8 inhibited the migration of PC-3-TRPM8 cells (P0.01) through the inactivation of focal-adhesion kinase.Conclusions It appears that TRPM8 was not essential for the survival of PC-3 cells;however,the overexpression of TRPM8 had negative effects on the proliferation and migration of PC-3 cells.Thus,TRPM8 and its agonists may serve as important targets for the treatment of prostate cancer.
9 citations
Journal Article•
TL;DR: Bilateral renal with cystic and solid mass the disease should be considered and focal excision with nephron sparing surgery is the primary therapy and selection pregnancy with gene detection is critical to prevent it occurrence and heredity.
Abstract: Objective To improve the diagnosis and treatment of von Hippel-Lindau syndrome.Methods Retrograde analyze the diagnosis and treatment of 8 cases of von Hippel-Lindau syndrome,4 received the operations of bilateral focal excision and renal cyst decortications,2 received unilateral focal excision and 2 only received regular follow-up without any operation.Results There were 4 individuals recovery,3 patients survival with tumor and 1 case death.Conclusions Bilateral renal with cystic and solid mass the disease should be considered,focal excision with nephron sparing surgery is the primary therapy and selection pregnancy with gene detection is critical to prevent it occurrence and heredity.
2 citations
Journal Article•
TL;DR: AHH combined with intraoperative autologousBlood transfusion make 44 patients avoid allogeneic blood transfusion in operation, and 38 patients had not underwent blood transfusions during the operation.
Abstract: Objective To study the effect of acute hypervolemic hemodilution(AHH) in pheochromocytoma operation.Methods AHH was performed in 53 pheochromocytoma patients,combined with autologous blood transfusion in 6 patients.Results AHH combined with intraoperative autologous blood transfusion make 44 patients avoid allogeneic blood transfusion in operation,and 38 patients had not underwent blood transfusion during the operation.Conclusions AHH combined with autologous blood transfusion may save medical cost,reduce the complications caused by allogeneic blood transfusion.
1 citations
Journal Article•
TL;DR: The surgery might be an effective method for treatment of this group of complex pheochromocytoma and clear position diagnosis, adequate drug preparation, andorous anesthesia care for developing successful surgery is very important.
Abstract: Objective To highlight the diagnostic and therapeutic procedures for complex pheochromocytoma. Methods The clinical data of 115 cases of complex pheochromocytoma from 1996 to 2008 were reviewed.There were 64 males and 51 females with an age range from 13 to 73 years(mean 42 years) in this cohort.Hypertension was observed in 92 cases(80%) and elevated 24 h urine catecholamine was observed in 82 cases(73.8%).The positive rate of localization of 131I-MIBG and Tc-99m-Otretide scintigraphy were 86.7%.The positive rate of CT and MRI were 100%. Results 109 cases received surgery,with 89 cases in open surgery,18 cases in laparoscopy,1 case in thoracoscope and 1 case in transurethral resection of submucous pheochromocytoma in bladder wall.No cases of death of surgery.The tumors were intraadrenal in 27 cases(diameter of 8.0-17.0 cm,average 12.0 cm) and extraadrenal in 82 cases(diameter of 1.5-22.0 cm,average 6.8 cm).Tumor location:64 cases in retroperitonaeum,7 cases in bladder,4 cases in mediastinum,5 cases in heart,1 case in neck and 1 case in liver.Range of follow-up was 1-144 months and follow-up was available for 101 of the 115 patients.There were 17 cases of recurrence or metastasis and 8 cases of death. Conclusions The diagnosis and treatment procedures of pheochromocytoma are complex.The surgery might be an effective method for treatment of this group of complex pheochromocytoma.Clear position diagnosis,adequate drug preparation,rigorous anesthesia care for developing successful surgery is very important.
1 citations