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Showing papers in "Journal of Genetic Engineering and Biotechnology in 2013"


Journal ArticleDOI
TL;DR: Chitinase enzyme has received increased attention due to its wide range of biotechnological applications, especially in agriculture for biocontrol of phytopathogenic fungi and harmful insects, and out of metal ions Mn 2+ and Cu 2+ enhanced enzyme production in the case of HS6, however, Co 2+ was the most appropriate for HS4.

182 citations


Journal ArticleDOI
TL;DR: In this article, the authors evaluated the antioxidant and antimicrobial properties of nutmeg (Myristica fragrans Houtt) seed extracts and found that the extract showed 93.12% and 74.94% inhibition of β-carotene bleaching, at 1 ǫ/mL extract concentration.

165 citations


Journal ArticleDOI
TL;DR: The enzyme was found to be serine active as it had been affected by lower concentrations of phenylmethylsulfonyl fluoride (PMSF) and complete dehairing of the enzyme-treated skin was achieved in 12 h, at room temperature.

70 citations


Journal ArticleDOI
TL;DR: Studies on the effect of different carbon and nitrogen sources revealed that xylose and urea enhances the enzyme production, and with selected C–N sources along with 1 M NaCl the maximum protease production was obtained.

67 citations


Journal ArticleDOI
TL;DR: In this article, the authors demonstrate the metabolism of 1,2 propandiol by Lactobacillus reuteri and elucidate the metabolites produced during the process using HPLC.

26 citations


Journal ArticleDOI
TL;DR: In vitro screening among the cultivars showed significant differences in the root length, shoot length and root/shoot ratio, and the presence of the drought genes and their chromosomal location was investigated by isolating and sequencing the dehydration responsive element binding protein (dreb1).

26 citations


Journal ArticleDOI
TL;DR: The aim of this study was to compare the potential of Ficus sycomorus latex peroxidase (POL) and horseradish peroxIDase (HRP) in the decolorization of a wide spectrum of eight synthetic dyes and two natural dyes, hibiscus flower color and pomegranate juice.

23 citations


Journal ArticleDOI
TL;DR: In this article, a statistical approach was used to study the optimization of media components for lipase production from Yarrowia lipolytica MTCC 35, where Mahua cake, glucose, MnCl 2 and KH 2 PO 4 were screened to be the most significant variables among the nine medium variables.

17 citations


Journal ArticleDOI
TL;DR: The reason why buffalo samples, analyzed by RFLP technique, using HindIII and HinfI used in cattle, were mistakenly identified as BB monomorphic isInvestigation of genetic polymorphism k-casein gene investigated in lactating Egyptian buf- falo shows that Egyptian buffalo has both alleles A and B with allelic frequencies of 0.57.

11 citations


Journal ArticleDOI
TL;DR: In this study, nine different methods of genomic DNA isolation were used and it was found that the BSA was the best among them followed by 10% glycerol, which enhances the amplification of the low quality DNA.

11 citations


Journal ArticleDOI
TL;DR: A suitable alternative facile route is reported, where cyanobacterial extracellular products are utilized as growth regulators and its performance validated on Gossypium hirsutum L. acuminata, which is best responsible for regeneration in cotton plants.

Journal ArticleDOI
TL;DR: The presence of 2 variants 178 Ser (T C A) and 178 Leu (T T A) in Egyptian buffalo CSN1S1 gene is reported for the first time.

Journal ArticleDOI
TL;DR: The results showed that GY/P was significantly and positively correlated with EC%, plant regeneration and RGP response and its association with heading date and grain yield per plant and the magnitudes of additive genetic variance were larger than those of non-additive ones for all studied traits.

Journal ArticleDOI
TL;DR: Recovery of intracellular enzymes was enhanced by using different concentrations of sodium cholate and deoxycholate, and sorbitol proved to be an efficient lyoprotectant.

Journal ArticleDOI
TL;DR: Results of this study showed that both glucose and PHA rich P. vulgaris extract have positive effects and enhance the growth and protein synthesis.

Journal ArticleDOI
TL;DR: This study aimed to detect the genetic polymorphism of as1- and as2-Casein genes in three native Egyptian sheep breeds; Rahmani, Barki and Ossimi using PCR-SSCP and PCR-RFLP and revealed the presence of a single nucleotide polymorphism (A fi G) in intron 6 of as 2-CN gene.

Journal ArticleDOI
TL;DR: Although the prospects of exploiting such a strategy for crop improvement are limited, the results provide further insight into the control of the quality over the storage period at the molecular level.

Journal ArticleDOI
TL;DR: Insulin resistance is a common finding in CHC, it is associated with increase liver steatosis, however it has no impact on EVR to combined interferon ribavirin therapy, viral load or necroinflammation.

Journal ArticleDOI
TL;DR: The utility of Immunofluorescence staining as a guide to confirm the diagnosis of MDCMD especially when molecular diagnosis is not available is demonstrated.