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Showing papers in "Journal of Immunological Methods in 1983"


Journal ArticleDOI
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.

50,114 citations


Journal ArticleDOI
TL;DR: The kinetic determination of the amount of the enzyme lactate dehydrogenase released from lysed target cells was determined to provide a sensitive and precise measure of natural cytotoxicity when used in conjunction with appropriate controls and calculational methods.

1,093 citations


Journal ArticleDOI
TL;DR: A solid-phase enzyme-linked immunosorbent assay (ELISPOT) is described, which provides a useful alternative to conventional plaque-forming cell assays.

1,047 citations


Journal ArticleDOI
TL;DR: The use of protein A from S. aureus as an anti-IgG reagent in immunological techniques has extended in recent years, together with knowledge about its interaction with immunoglobulins of different species.

562 citations


Journal ArticleDOI
TL;DR: A sensitive immunoassay is described for the detection of idiotype- and isotype-specific antibody-secreting cells (ASC), based upon the well established principles of ELISA, which has sensitivity and specificity at least equivalent to haemolytic plaque assays.

556 citations


Journal ArticleDOI
TL;DR: The time course of H2O2 release by mouse peritoneal and bone marrow-derived macrophages and by human neutrophils was determined following stimulation with zymosan particles or phorbol myristate acetate, and the dependence of H1N1 release on cell number and stimulus dosage was studied.

272 citations


Journal ArticleDOI
TL;DR: A method of coupling the strong chelator diethylenetriaminepentaacetic acid (DTPA) to IgG antibody is developed which is simple, efficient, and superior to reported methods.

254 citations


Journal ArticleDOI
TL;DR: The biotin/avidin system was incorporated into the enzyme-linked immunosorbent assay (ELISA) technique to increase the sensitivity of the standard ELISA for the detection of mouse antibody to hepatitis B surface antigen.

248 citations


Journal ArticleDOI
TL;DR: An ELISA double antibody binding system has been developed and it is shown that, of the 7 anti-beta 2 monoclonal antibodies obtained, 5 recognize the same antigenic site and the 2 others recognize a second site.

233 citations


Journal ArticleDOI
TL;DR: The effects of peptic digestion on mouse monoclonal immunoglobulins of each subclass of IgG were investigated and five different IgG2b proteins were degraded very rapidly without the detectable formation of antigen-binding F(ab')2 fragments.

222 citations


Journal ArticleDOI
TL;DR: A simple and efficient technique to purify human peripheral blood monocytes is described, based on the fact that monocytes have high affinity for fibronectin immobilized on a gelatin coated surface.

Journal ArticleDOI
TL;DR: The technique offers a simple method of quantifying ingested protein aggregates and of studying heterogeneity in phagocyte populations, and was found to be the best quenching agent of extracellular fluorescence when using ingested aggregated IgG.


Journal ArticleDOI
TL;DR: This microassay may be a useful substitute for macro and semi-micro tests for colorimetric determination of serum complement activity and for microassays based on the release of a radio-isotope.

Journal ArticleDOI
TL;DR: This method was simple, very effective, resulting in essentially complete removal of several endotoxins from heavily contaminated solutions (1-10 micrograms/ml by Limulus amoebocyte lysate assay) and employed mild physiological conditions.

Journal ArticleDOI
TL;DR: A reproducible activity of mature macrophages against K562 and MOLT4 tumor cells was revealed and the ability to inhibit growth of human permanent tumor cell lines also developed during macrophage maturation.

Journal ArticleDOI
TL;DR: A simple method was devised for counting small numbers (10(4)-10(6) of adherent mononuclear phagocytes, including populations containing multinucleated giant cells, which often arise during cultivation of human blood monocytes.

Journal ArticleDOI
TL;DR: A micro enzyme-linked immunosorbent assay utilizing antigen dotted onto nitrocellulose filter discs (Dot-ELISA) was developed for the rapid diagnosis of visceral leishmaniasis and should prove to be an important field diagnostic technique for visceral leishes.

Journal ArticleDOI
TL;DR: The optimal conditions for the preparation of Fab and F(ab')2 fragments from monoclonal rat IgG of different subclasses are described and Fab fragments are isolated by sequential chromatography on Ultrogel AcA 44 and on DEAE-cellulose columns.

Journal ArticleDOI
TL;DR: When compared in an ELISA with conventional reagents, the battery of monoclonal antibodies proved to be as sensitive as conventional polyclonal antibodies.

Journal ArticleDOI
TL;DR: A colorimetric method for detecting the lysis of target cells by cytotoxic T lymphocytes (Tc) was developed in this paper, which is substantially more sensitive than the 51Cr release assay and can be used to detect alloreactive Tc cells and H-2-restricted TC cells against viruses, haptens and minor-H antigens.

Journal ArticleDOI
TL;DR: Stability of Ig production during continuous cultivation in the protein-free medium over an extended period was studied for a myeloma and a hybridoma line, both of which produced high levels (50–200 μg/ml) of Ig for periods of 11 and 22 weeks, respectively.

Journal ArticleDOI
TL;DR: Measurement of the binding of constant trace amounts of labelled antigen by increasing dilutions of culture supernatant allows the ranking of monoclonal antibodies in the order of their affinity for the antigen.

Journal ArticleDOI
TL;DR: Experiments were performed to determine whether for mice pre-injected with pristane there is an optimum interval before injection of hybridomas in order to maximize ascites fluid formation and yield of monoclonal antibodies.

Journal ArticleDOI
TL;DR: The AB-microELISA is not only rapid and inexpensive, but also more sensitive than other published ELISA procedures and comparable to solid-phase radioimmunoassays in the quantitation of total and allergen-specific IgE.

Journal ArticleDOI
TL;DR: An enzyme-linked immunosorbent assay has been developed for quantitation of lactoferrin (LF) in body fluids and advantages include its rapidity, and radioactivity is not required.

Journal ArticleDOI
TL;DR: Suspension cultures of B cell hybridomas can be maintained in exponential growth, by matching their rate of replication with the input of medium, and the yield of immunoglobulin is a direct function of the number of live cells and does not depend on their rate growth.

Journal ArticleDOI
TL;DR: The ingestion of fixed erythrocytes by murine macrophages can be monitored by fluorescence microscopy after the addition of 1 mg/ml of trypan blue at the end of the assay.

Journal ArticleDOI
TL;DR: Assay systems utilizing biotinylated antibody and avidin-biotin complex can provide for sensitive, specific assays for the measurement of microbial antigens.

Journal ArticleDOI
TL;DR: The present studies investigate the potential of simultaneous multiple determinations of specific cell surface antigens in one reaction incubation by employing orderly arranged antibody spots on a solid surface found to be capable of serving as minute specific immunoadsorbents for cells bearing on their surface the antigen with which the antibodies reacted.