Journal of Laboratory and Clinical Medicine 

About: Journal of Laboratory and Clinical Medicine is an academic journal. The journal publishes majorly in the area(s): Platelet & Blood serum. It has an ISSN identifier of 0022-2143. Over the lifetime, 12921 publication(s) have been published receiving 396565 citation(s).

Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase

Abstract: An assay procedure is described in which blood cell glutathione peroxidase may be accurately measured by a direct spectrophotometric procedure. Glutathione peroxidase activity is found to be associated with a relatively stable, nondialyzable, heat-labile, intracellular component which is separable from hemoglobin, by gel filtration and ammonium sulfate precipitation. The activity appears to be dependent upon active sulfhydryl groups and is unaffected by low concentrations of azide, cyanide, or ferricyanide.

Two simple media for the demonstration of pyocyanin and fluorescin.

Abstract: Two simple media for the enhancement of pigment production by certain organisms of the Pseudomonas genus are described. The results of comparative studies employing these media, certain synthetic broths, and some commonly used dehydrated preparations are reported. One hundred (93 per cent) of the 107 strains of Pseudomonas studied were detected by pigment production on medium B; all 107 strains could be detected by the combined use of Heart Infusion agar and 27 per cent on Tryptose agar. The reactions produced strains elaborated a water-soluble pigment; 40 per cent were positive on Difco media A and B. On Difco Proteose peptone No. 3 agar, 77 per cent of the same on the two media by organisms belonging to certain other genera are described.

A new method for the direct determination of serum cholesterol.

Abstract: A direct method for the quantitative determination of total serum cholesterol is described. The procedure consists of adding a reagent containing sulfuric acid, acetic acid, and ferric chloride to a 0.1 ml. sample of serum. The sensitivity achieved is several times that of current procedures and the time required for a single determination is in the order of minutes. Precision and reproducibility are demonstrated as well as the absorption characteristics of the purple color produced through the spectral range of 400 to 700 mμ. Finally, the method is compared with samples in which total cholesterol is determined by the Kingsley-Schaffert and Schoenheimer-Sperry procedures.