Showing papers in "Journal of Lipid Research in 1962"

TL;DR: In this paper, a two-dimensional thin-layer chromatography (TLC) was developed that separated rat liver phosphatides into several phosphate-positive spots in about two hours developing time.

TL;DR: The liver appears to be the chief source of circulating triglyceride fatty acids (TGFA) and the rate of hepatic lipogenesis in fasting animals is very low, whereas influx of free fatty acids into the liver is great, and it appears that the chief sources of circulating FFA and TGFA are discussed.

TL;DR: In this article, a method for the separation of free fatty acids from other lipids is presented, where the sample is introduced in ethyl ether, and neutral lipids are eluted with this solvent.

TL;DR: Thin-layer chromatography of phospholipids and cerebrosides was performed on Silica Gel G plates using a mixture of chloroform-methanol-acetic acid-water as development solvent and two types of chromatoplates used: “neutral” and “basic” plates.

TL;DR: A process as vital to caloric balance as fat synthesis must be precisely regulated to meet the everchanging energy needs of the organism.

TL;DR: In this article, the aldehydogenic chains of human erythrocyte plasmalogens were isolated as a group and converted to their aldehyde, alcohol, acetylated alcohol, fatty acid methyl ester, and dimethylacetal derivatives; similar derivatives were prepared from reference fatty acids.

TL;DR: In this paper, the liver oils of the grey dogfish, the Greenland shark, and the ratfish were isolated by chromatography on alumina and then submitted to gas-liquid chromatography (GLC).

TL;DR: The mechanism of the incorporation of plasma triglyceride fatty acids (TGFA) into adipose tissue was investigated in this article, where they were incubated under various conditions with plasma very low-density lipoproteins (d

TL;DR: The observations suggest that, in derivatives of phenylethylamine, hydroxyl substitution at the pura position and on the @-carbon and the presence of a primary or secondary amine structure are associated with the potent ability to elicit elevations of FFA in man.

TL;DR: The liver triglycerides showed a C14/H3 ratio close to 1.0 during the first 10 min, but then declined rapidly as discussed by the authors, and by 20 min, when most of the chylomicron glyceride had disappeared from the blood, 35% of the recovered fatty acid radioactivity was found in the liver and 10% in the adipose tissue.

TL;DR: The glyceryl ethers in man and cow were generally much more saturated than those in the liver oils of elasmobranch fish, although a fairly high degree of unsaturation was found in the glyceryL ethers of human milk.

TL;DR: When fat-body tissue from locusts was incubated with palmitate-1-C14 in phosphate-saline, the acid was readily taken up by the tissue; 80-90% of that taken up was esterified and recovered in the glyceride fraction.

TL;DR: In this article, the hydroxamic acid reaction conditions for the determination of esterified fatty acids were modified to the extent that the variable factors involved were controlled so that the molar absorptivities per ester group for triglycerides, cholesteryl esters of long-chain fatty acids, and phosphatides were equivalent through 8 microequivalent esters.

TL;DR: The suggestion is made that this gland stores specific sterol esters and hydrolyzes chylomicron esters to resynthesize the cholesterol esters characteristic of the gland.

TL;DR: The lipid composition of normal Swiss laboratory mouse liver has been determined by chromatography and infrared spectrophotometry and a trace of lysolecithin was also detected, although it was impossible to determine whether or not it was an artifact of the isolation procedure.

TL;DR: The principal lipid of A. agilis, A. tumefaciens, and E. coli extractable with ethanol and methanol-chloroform was a phosphatidyl ethanolamine as mentioned in this paper.

TL;DR: In this article, the composition of normal human bone marrow has been determined, which is composed primarily of neutral lipid and contains less than 3% of phospholipid and no detectable carbohydrate.

TL;DR: It was found that the proportion of total incorporated radioactivity in the monounsaturated acids was significantly higher in tissue taken from young animals than in tissues taken from old animals, when the rats were fed a fat-free diet.

TL;DR: Of the three inositol phosphatides separated by chromatography on formaldehyde-treated paper, monophosphoinositide always had the lowest specific activity; after 16 and 32 hours, phosphoinositides 2 and 1, which is probably identical with the triphosphoinposide of Ballou, had the highest specific activity.

TL;DR: In this paper, a method for radioassay of carbon-I4 labeled substances analyzed by gas-liquid chromatography is described, where column effluent is automatically fractionated by condensing the vapor in a series of cartridges filled with anthracene crystals coated with silicone oil.

TL;DR: The data on effect of enzyme concentration on hydrolysis rate suggest that a second component, perhaps an emulsifier, is required for activity, and there may be two separate enzymes for the short-chain and long-chain triglycerides, respectively.