Showing papers in "Journal of Lipid Research in 1964"

TL;DR: The procedure developed is simple, rapid, and generally applicable t o lipids, and the results did not affect the validity of the method.

TL;DR: A chemical reagent spray specific for phosphate esters, based on molybdenum blue, gives an instantaneous, specific reaction with phospholipids on silica gel or alumina thin layer chromatography (TLC) plates.

TL;DR: Human red cell lipids have been studied in a series of normal individuals and evidence is presented that the total amount of lipid phosphorus per average red cell is 1.40 X lo-" mg and that most extraction procedures fail to extract 870 or more of red cell phospholipid.

TL;DR: In this paper, the authors described methods for separation of all known prostaglandins and some derivatives as free acids and methyl esters by thin-layer chromatography both on analytical and preparative scales.

TL;DR: A review of the role of sterols in insect physiology can be found in this paper, where the authors show that the sterols per se have diverse roles within the tissues, which for convenience may be described as "structural" and metabolic functions are fulfilled.

TL;DR: In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.

TL;DR: In the presence of a known excess of acetyl CoA, free CoA released was determined simultaneously in a coupled spectrophotometric assay and CoA formed was shown to be directly related to carnitine initially present.

TL;DR: The possible effect of the two diets on the excretion of “nondietary” cholesterol as neutral fecal steroids is discussed.

TL;DR: This work has chosen to focus upon metabolicevents occurring in the epithelial cells of the gut mucosa, and a deviation has been made from the usual anatomic sequence of discussion in order to consider the glycerol moiety of theglycerides synthesized in the cells, the activation of fatty acids, and the pathway of direct esterification of monoglycerides.

TL;DR: E. H. HORNING, Baylor University College of Medicine, Houston, Teras (Chairman) and E. F. LIPSKY, Yale University School of medicine, New Haven, Connecticut (Program Officer), National Heart Institute, Uational Institutes of Health, Bethesda 14, Maryland.

TL;DR: A method for the complete separation and essentially quantitative isolation of individual components of phospholipid mixtures is described, using Silica Gel G as adsorbent and solvent mixture containing formic acid.

TL;DR: The fatty acid composition of each sphingolipid was determined by gas-liquid chromatography, and both odd and even chain saturated and monounsaturated fatty acids containing 14 to 26 carbons were present.

TL;DR: In this paper, the specificities of the acyl transferase reactions leading to diacyl glycerol phosphate are not adequate to provide the pattern of fatty acids that is known to occur in glyceroliipids in nature.

TL;DR: The quantities of ethanolamme glycerophosphatides (EGP), choline glycerphosphatide (CGP), and serine glycerophile (SGP) were determined in the grey and white matter from three apparently normal adult hiiman brains, and in each locale the ouantities decreased from EGP through CGP to SGP.

TL;DR: A hitherto unreported esterified form of steryl glucoside has been isolated from potato tuber lipids and soybean phosphatides as discussed by the authors, which is composed of sterol, glucose, and fatty acid in the molar ratio 1:1:1.

TL;DR: In this paper, permanent records of thin-layer chromatograms are obtained by a simple method employing blueprint paper and not requiring special equipment, which is a technique similar to the one described in this paper.

TL;DR: In this paper, the melting point of the lipid of the planktonic copepods remained during the whole year somewhat lower than the water temperature, and the proportions of the C20-C22 poly-unsaturated acids in the crustaceans increased with decreasing temperature and in some species exceeded the values characteristic of marine animals.

TL;DR: In this article, a multicompartmental model of plasma and liver triglyceride metabolism was constructed which was compatible with the known physiological and biochemical behavior of triglycerides and the observed data, and the rates of triglyceride transfer from one compartment to another and the compartment sizes were calculated using a digital computer.

TL;DR: Using a double isotopic steady state in animals fed cholesterol-7a-H3 and implanted with cholester01-4-C’~, it has been possible to demonstrate that the major portion of fecal cholesterol of endogenous origin is derived from or is in equilibrium with that of the blood.

TL;DR: Incorporation of C14-labeled fatty acids into phospholipids by washed ghosts, prepared from human erythrocytes, required the addition of ATP and was markedly stimulated by MgC12 and coenzyme A.

TL;DR: In this article, a method was described for extracting virtually all of the endogenous substrate with ether, after which about 25% of the original hormone-sensitive enzyme activity can be recovered in an insoluble fraction that now sediments.

TL;DR: The positional and fatty acid specificities of lipases from several different microorganisms have been examined by gas-liquid chromatographic analysis of the free fatty acids following action on mixed triglycerides of known composition.

TL;DR: Comparison of Cerebroside and cerebroside sulfate isolated from the same source revealed a similar fatty acid composition for these glycolipids, both in the normal and hydroxy series.

TL;DR: The physiological implication of these results seems to be that the transformations catalyzed by pancreatic lipase in the presence of bile salt form a system for the continuous supply of monoglycerides and fatty acids to the brush border of the intestinal cells.

TL;DR: The results suggest that in the postabsorptive state each lipid class originates from the same source in all three lipoproteins.

TL;DR: The complete structures of 51 fatty acids of E. gracilis have been determined as mentioned in this paper, including saturated fatty acids from C9 to C19, monounsaturated fatty acid from C 13 to C 19, diunsaturated fatty acids, tri-unsaturated acids, tetraunsaturated acid, pentaunsaturated acid and one docosahexaenoic acid.

TL;DR: No direct correlation could be established between the serum lipid levels and the extent and distribution of intravascular thrombosis and necrosis in the lungs, liver, and spleen, or the production of the generalized Shwartzman phenomenon in the kidney, but there appeared to be a direct correlation between the elevated serum triglyceride levels and

TL;DR: In this article, the Clark-Stetten model of hormone action was used to evaluate the effect of various Amino Amines and adrenocorticotropin (ACTH) on fat cells in hamster adipose tissue.

TL;DR: The plant sulfolipid has been isolated from Chlorella pyrenoidosa cells and from alfalfa leaves by chromatography on Florisil and DEAE cellulose columns as discussed by the authors.